Tissue Banks

这一共享资源的目的是在专家病理学家的指导下，为所有项目的研究人员提供人体组织、生物液体和专家病理解释。组织病理学和组织/生物流体资源自1986年以来一直存在，并在GI孢子的支持下扩大。截至今年，该资源包括1698份结直肠癌切除样本、354份结直肠癌腺瘤样本、325份结直肠癌肝转移样本、1013份胰腺癌样本、588份结直肠癌移植瘤样本、101份胰腺癌移植瘤样本、5298份来自结直肠癌高危人群的血液样本，以及867份来自胰腺癌患者的血液样本。绝大多数切除标本都有福尔马林固定的石蜡包埋组织、冰冻材料以及临床和结果数据。最近，该资源扩大了收集范围，包括来自120名快速尸检参与者的高质量速冻和福尔马林固定的终末期胰腺癌或结直肠癌样本。已经创建了133个结直肠和胰腺肿瘤和疾病的组织微阵列。从人类肿瘤中制备了8个完全特征化的胰腺癌细胞系，并与ATCC共享。这些生物标本是根据国家癌症研究所的生物仓库最佳实践指南进行收获和储存的。将这些标本分发给孢子研究人员已出版了100多份出版物。该中心获取更多的异种移植、新鲜冷冻的结直肠癌和胰腺肿瘤以及血液和胰液样本，并为研究人员提供专家病理咨询。标本的收集是在具有结直肠和胰腺肿瘤专业知识的病理学家的监督下进行的，与这些领域的临床专家密切合作，并与基础研究调查人员密切合作，以最大限度地发挥项目的翻译影响。临床和家族病史被输入到一个受密码保护的基于网络的跟踪系统中，用于我们的胰腺病例。这个基于Web的界面遵循国家研究委员会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。已经建立了一种机制，以确定向约翰霍普金斯大学GI癌症孢子内外的调查人员分配所需资源的优先顺序。生物样本已经与其他50多个机构的调查人员共享。与其他中心的研究人员合作，促进了罕见肿瘤类型的增加。

The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 1698 banked colorectal carcinoma resection specimens, 354 colorectal adenoma samples, 325 hepatic metastases of colorectal carcinoma, 1013 pancreatic carcinoma specimens, 588 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 5298 blood specimens from individuals at risk for colorectal carcinoma, and 867 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. Most recently, this resource has expanded its collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 120 rapid autopsy participants. 133 tissue microarrays of colorectal and pancreatic neoplasms and diseases have been created. Eight fully characterized pancreatic carcinoma cell lines have been prepared from human tumors and shared with the ATCC. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 100 publications. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. Clinical and family histories are entered into a password protected web-based tracking system for our pancreatic cases. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions. Collaboration with investigators at other centers fosters the accrual of rare tumor types.