Triple Analysis of Blood Clotting using Driven and Diffusing Microbeads

使用驱动和扩散微珠对血液凝固进行三重分析

• 批准号: 8253139
• 负责人: Richard Chasen Spero
• 金额: $ 24.11万
• 依托单位: REDBUD LABS, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8253139
• 关键词: Adopted; Adoption; Animal Model; Area; Basic Science; Biological Assay; Blood; Blood Clot; Blood coagulation; Blood coagulation tests; Brain; Breathing; Cardiovascular system; Cause of Death; Chemicals; Childhood; Clinical; Clinical Research; Coagulation Process; Development; Diagnosis; Diagnostic; Diffuse; Diffusion; Disease; Electronics; Environment; Etiology; Event; Gel; Heart; Hematologist; Human Resources; Image; Kinetics; Legal patent; Lung; Magnetism; Market Research; Marketing; Measurement; Measures; Mechanics; Microscope; Microscopy; Microspheres; Normal Range; Optics; Pathologist; Patient Monitoring; Performance; Permeability; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Physics; Plasma; Population; Population Study; Positioning Attribute; Price; Protocols documentation; Qualifying; Research; Resolution; Sampling; Scientific Advances and Accomplishments; Small Business Innovation Research Grant; Speed; Structure; System; Systems Development; Techniques; Technology; Testing; Time; Work; base; commercialization; cost; design; drug discovery; high throughput screening; innovation; instrument; nanoparticle; novel; prototype; research study; success; theories

DESCRIPTION (provided by applicant): Population studies and drug research require a high-throughput measurement of clot stiffness, permeability. The single largest cause of death in the US over the latter half of the 20 century was and still is a thrombotic event: a clot in the heart, brain, or lungs. Assessing clot quality is essential for studying, diagnosing, and treating disease Conventional blood coagulation tests can be grouped into three areas: clotting time, mechanical stability, and permeability. Clot stiffness and permeability are correlated with a broad range of diseases, but because they are not available in high throughput screening (HTS) format, the tests are under-applied. An HTS system for these tests would illuminate the relationship of disease etiology and abnormalities of blood clots, enable large- population screens, and speed development of new pharmaceuticals. We anticipate that these measurements will one day provide superior clinical monitoring for patients receiving treatment that modifies thrombotic activity. With this Phase 1 SHIFT SBIR Rheomics will develop a triple-analysis of clotting that uses small beads. Clotting time is measured by seeing microbeads (1-5 5m) become trapped in a forming clot. Mechanical Stability is measured by tugging on microbeads using our patented magnetic force technology. Permeability is measured using a novel technique invented by the PI/PD, in which hindered diffusion of nanoparticles is used to derive the clot's permeability by applying the effective medium theory. This Phase 1 SBIR will test the feasibility of an HTS "triple-analysis" that will simultaneously and automatically measure clot kinetics, strength, and permeability. This Phase 1 SBIR comprises two specific aims: assembling an imaging/magnetics platform to perform the tests, and validating the tests against standard coagulometers. We are aware of no existing system (commercial or otherwise) that can perform all three measurements, let alone perform them simultaneously, automatically, or in high throughput. In success, the Rheomics triple-analysis will deliver a stiffness assay that, unlike existing techniques, can be scaled to high throughput (eg, 96-well tray) format, and a permeability assay that is up to 60x faster, fully automated, and uses small sample volumes. Rheomics is uniquely positioned to deliver on these aims. Our team features an intersection of expertise in optical and magnetic systems development, soft-matter physics, and technology commercialization, including key personnel who patented, developed, and transferred for commercialization the technology behind the widely adopted CoaguChek(R) System, and who founded Cardiovascular Diagnostics, a successful IPO. PUBLIC HEALTH RELEVANCE: Rheomics is developing a coagulometer that will simultaneously measure clotting time, clot stiffness, and clot permeability in a high throughput, small volume format that is up to 60x faster than existing techniques. This system will advance scientific research into blood clot structure and function, enable large-population clinical research studies, and speed development of new pharmaceuticals.

描述（由申请人提供）：人口研究和药物研究需要高通量测量凝块硬度，渗透性。在20世纪后半叶，美国最大的死亡原因一直是血栓形成事件：心脏中的血块，