Muscle/Bone Phenotyping Core

instnictions）： 肌肉/骨骼表型核心的目标是支持所有子项目的研究资助， 程序项目。该计划项目的总体目标是了解 肌肉和骨骼之间的相互作用，可能导致肌肉和骨骼质量的年龄相关下降 和功能每个次级项目从不同的角度处理这一全球问题，包括： 肌肉对成骨细胞和骨细胞功能的影响（子项目1）; 老化过程中的肌肉功能（子项目2）;老化过程中成骨细胞动力学的骨细胞控制（子项目 3）;以及老化对骨细胞对机械刺激反应的影响（子项目4）。肌肉/骨骼 表型分析核心将为所有子项目提供表型分析支持，包括体内和体外 骨骼组织的X射线、光密度和microCT分析，肌肉的组织学制备和染色 和矿化组织，定量组织形态计量学和动态骨和肌肉组织形态计量学， 免疫组织化学和原位杂交。这些技术对于所有的子项目都是必不可少的， 其肌肉和骨骼表型被表征为转基因小鼠中年龄的函数 骨细胞或肌肉功能改变的模型，以及受到机械损伤的小鼠， 加载中除了提供这些传感器外，核心还将提供标准化的样本方案， 准备和核心内的所有方法，以保持跨子项目的一致性。芯会 也作为一个资源，为学生，博士后和研究提供上述技术的培训 在子项目1、2、3和4内进行研究的人员。通过集中这些表型分析方法 在一个核心中，我们将在所有子项目中标准化这些技术，加快 研究，加强学生和博士后的教育，使研究能够在一个 更具成本效益的方式。 相关性（参见说明）： 该研究核心将为一个计划项目中的四个子项目提供集中支持， 是了解肌肉和骨骼相互作用的机制，这些机制有助于与年龄相关的 肌肉和骨骼质量下降。该核心将标准化肌肉和骨骼的分析方法， 所有项目中使用的鼠标模型。这项研究可能会导致新的治疗骨质减少/sarcopenla。

instnictions): The goal of the Muscle/Bone Phenotyping Core is to support the research alms of all subprojects within the program project. The overall aim of the program project is to understand the mechanisms underiying crosstalk between muscle and bone that may contribute to the age related decline In muscle and bone mass and function. This global question is addressed by each subproject from a different perspective, including: the effects of muscle on osteoblast and osteocyte function with aging (subproject 1); osteocyte modulation of muscle function during aging (subproject 2); osteocyte control of osteoblast dynamics with aging (subproject 3); and effects of aging on osteocyte responses to mechanical stimulation (subproject 4). The Muscle/Bone Phenotyping Core will provide phenotyping support for all subprojects, which will include In vivo and ex vivo X-ray, densitometric and microCT analysis of skeletal tissues, histological preparation and staining of muscle and mineralized tissues, quantitative histomorphometry and dynamic bone and muscle histomorphometry, immunohistochemistry and in situ hybridization. These techniques are essential for all the subprojects in which muscle and bone phenotypes are being characterized as a function of age in transgenic mouse models with altered osteocyte or muscle function, as well as mice that have been subjected to mechanical loading. In addition to providing these sen/lces the core will provide standardized protocols for sample preparation and for all methods within the core to maintain consistency across the subprojects. The core will also act as a resource to provide training in the above techniques for students, postdocs and research personnel conducting research within subprojects 1, 2, 3 and 4. By centralizing these phenotyping methods within a single core, we will standardize these techniques across all the subprojects, accelerate the pace of the research, enhance the education of students and postdocs and enable the research to be completed in a more cost-effective manner. RELEVANCE (See instructions): This research core will provide centralized support for four subprojects within a program project whose goal is to understand the mechanisms underiying muscle and bone interactions that contribute to age-related decline in muscle and bone mass. The core will standardize methods for analysis of muscle and bone in the mouse models used in all projects. This research may lead to new treatments for osteopenia/sarcopenla.