Genomics & Molecular Engineering Core

基因组学

• 批准号: 8377910
• 负责人: Nathan A. Ellis
• 金额: $ 20.3万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8377910
• 关键词: Advisory Committees; African American; Architecture; Asthma; Bacterial Artificial Chromosomes; Basic Science; Biochemistry; Biological Models; Cells; Charge; Chicago; Chromosomes, Human, Pair 3; Clinical; Clinical Trials; Colorectal Cancer; Committee Membership; Communication; Complex; Consultations; Core Facility; Cost Savings; Crohn' s disease; DNA; DNA Sequence; DNA Sequence Analysis; Data; Databases; Development; Digestive System Disorders; Disease; Disease Outcome; Ectopic Expression; Engineered Gene; Engineering; Ensure; Explosion; Faculty; Fee-for-Service Plans; Fostering; Future; Gastrointestinal tract structure; Gene Expression; Gene Silencing; Genes; Genetic; Genetic Engineering; Genetic Materials; Genetic Models; Genetic Polymorphism; Genetic Research; Genetic Services; Genetic Transcription; Genetic Variation; Genomics; Genotype; Grant; Human; Human Genome; Ice; Image; Image Analysis; Immunology; Individual; Inflammatory Bowel Diseases; Institution; Interdisciplinary Study; Intestinal Diseases; Knock-in Mouse; Knock-out; Knowledge; Leadership; Lentivirus Vector; Link; Mammalian Cell; Methodology; Methods; Microbe; Microbiology; Mission; Modality; Modems; Molecular; Molecular Biology; Mucositis; Mus; Mutation; Needs Assessment; Other Genetics; Outcome; Pathway interactions; Patients; Phenotype; Play; Population; Population Study; Preparation; Proteins; Proteomics; Reagent; Research; Research Personnel; Resource Sharing; Resources; Rheumatoid Arthritis; Risk; Risk Factors; Role; Rotation; Sampling; Scientific Advances and Accomplishments; Services; Severities; Single Nucleotide Polymorphism; Somatic Cell; Somatic Cell Genetics; Staging; Strategic Planning; System; Technology; Time; Tissues; Training; Translational Research; Ulcerative Colitis; Universities; Viral; base; cancer risk; cost; discount; empowered; functional genomics; gene function; genetic analysis; genetic manipulation; genetic technology; genome wide association study; homologous recombination; interest; meetings; member; new technology; novel; operation; protein expression; research study; treatment response

The Genomic and Molecular Engineering (GME) Core of the DDRCC provides cutting-edge technologies for the analysis and manipulation of the genetic material. These new technologies empower DDRCC members to pinpoint and characterize genetic factors that influence the development and course of digestive diseases and to dissect gene functions In important pathways relevant to digestive diseases. The GME is a new Core, restructured from the former Molecular Biology and Biochemistry Core. The GME Core divided into two components. The Genotype Analysis component offers sen/ices relating to the genetic analysis of patient samples. The services of the Genotype Analysis component Include (1) customized single nucleotide polymorphism (SNP) genotyping based on the Sequenom Massanray genotyping platfonn, (2) standard SNP genotyping panels for high-Interest genes, (3) ultra-high throughput DNA sequence analysis for genotyping, (4) other genotype analysis methods (such as TaqMan), (5) DNA preparation, and (6) statistical genetics support. The Genetic Engineering component offers services relating to the manipulation of genes in cellular and organismal model systems. The services ofthe Genetic Engineering component include (1) somatic cell genetic manipulation of genes using homologous recombination to knock in or knock out mutations, (2) recombineering technologies to manipulate large DNA segments in bacterial artificial chromosomes, (3) the construction of gene expression constructs using lentiviral vectors for ectopic expression or silencing of genes, (4) support for realtime PCR, and (5) support for the Odyssey image analysis system. The GME Core supports members for genotype analysis and genetic engineering experiments by supporting labor cost, providing discounts for reagents, and training members in new technologies. The Administrative Directors of the GME Core, Drs. Nathan Ellis and David Boone, oversee the operations of the respective components. Directors are responsible for ensuring proper scientific direction and efficient use of services and facilities of the component resources. Usage of the GME Core rapidly increased from start-up because of substantial cost savings, relevance, and high quality of services and resources, and it is anticipated usage will grow substantially during the next cycle of this Grant. Each of the Components offers training of new and established investigators unfamiliar with the supported experimental approaches. The GME Core has helped to foster multidisciplinary collaborations and promote productive exchanges brought about by sharing of resources

DDRCC的基因组和分子工程（GME）核心为遗传物质的分析和操作提供了前沿技术。这些新技术使DDRCC成员能够查明和表征影响消化系统疾病发展和过程的遗传因素，并解剖与消化系统疾病相关的重要途径中的基因功能。GME是一个新的核心，从原来的分子生物学和生物化学核心重组。GME核心分为两个部分。基因型分析组件提供与患者样本遗传分析相关的sen/ices。基因型分析组件的服务包括(1)基于Sequenom Massanray基因分型平台的定制单核苷酸多态性（SNP）基因分型；(2)高兴趣基因的标准SNP基因分型面板；(3)用于基因分型的超高通量DNA序列分析；(4)其他基因型分析方法（如TaqMan）； (5) DNA制备；(6)统计遗传学支持。