Microscale Tip Device for Rapid Concentration and Storage of PCR-ready DNA from B

用于快速浓缩和储存来自 B 的 PCR 就绪 DNA 的微型尖端装置

• 批准号: 8395181
• 负责人: Jae-Hyun Chung
• 金额: $ 15万
• 依托单位: NANOFACTURE, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8395181
• 关键词: Address; Biological Preservation; Blood; Blood capillaries; Blood specimen; Body Fluids; Centrifugation; Clinical; Collection; DNA; DNA analysis; Data; Development; Devices; Disposable Equipment; Environment; Feasibility Studies; Genetic; Genomics; Goals; Human; Human Genetics; Laboratories; Methodology; Methods; Normal Statistical Distribution; One-Step dentin bonding system; Performance; Phase; Plasma; Preparation; Protocols documentation; Public Sector; Reagent; Recovery; Reproducibility; Risk; Running; Saliva; Sampling; Site; Small Business Innovation Research Grant; Source; Specimen; Spottings; Staging; Swab; System; Testing; Time; United States National Institutes of Health; Whole Blood; Work; base; capillary; commercialization; cost; electric field; human DNA; innovative technologies; new technology; prototype; success

DESCRIPTION (provided by applicant): The current SBIR Phase I proposal addresses a need for the rapid and simple sample preparation of genomic DNA from human saliva and blood through a novel technology: microscale tip enrichment probes that employ electric fields and capillary action. With the action of a microtip-installed device, DNA contained in a given saliva sample can be selectively captured without centrifugation on the proposed microtip, and stored as a dried form for long- term preservation and delivery. The preserved DNA on microtips is released into a desired medium for PCR analysis without additional purification steps. Therefore, this innovative technology enables the one-step concentration of DNA from human saliva and blood as a purified, durable form. This application describes a feasibility study of the proposed method and system to explore and optimize the protocol of centrifuge-free preparation of PCR-ready DNA. PUBLIC HEALTH RELEVANCE: This proposal aims to conduct a feasibility study for a portable device capable of concentrating and purifying human genomic DNA from saliva without centrifuge. The success of the project would provide a convincing rationale and form a technical basis for the proposed methodology for rapid and simple preparation of PCR-ready DNA from various sample specimens with a high throughput at a low cost.

描述（由申请人提供）：目前的SBIR第I阶段提案解决了通过一种新技术（采用电场和毛细管作用的微尺度尖端富集探针）从人唾液和血液中快速简单制备基因组DNA样品的需求。通过微尖端安装装置的作用，给定唾液样品中包含的DNA可以在不离心的情况下被选择性地捕获在所提出的微尖端上，并以干燥形式储存用于长期保存和递送。将微尖上保存的DNA释放到所需的培养基中进行PCR分析，无需额外的纯化步骤。因此，这项创新技术能够一步浓缩人类唾液和血液中的DNA，使其成为纯化的持久形式。本申请描述了一个可行性研究， 提出的方法和系统，以探索和优化无离心制备PCR就绪DNA的方案。 公共卫生关系：该提案旨在对一种能够在不使用离心机的情况下从唾液中浓缩和纯化人类基因组DNA的便携式设备进行可行性研究。该项目的成功将提供一个令人信服的理由，并形成一个技术基础，为快速和简单的制备聚合酶链反应准备DNA从各种样品标本，高通量，低成本的拟议方法。

项目成果

Rapid extraction and preservation of genomic DNA from human samples.

从人类样本中快速提取和保存基因组 DNA。

• DOI: 10.1007/s00216-012-6637-8
• 发表时间: 2013
• 期刊: Analytical and bioanalytical chemistry
• 影响因子: 4.3
• 作者: Kalyanasundaram,D;Kim,J-H;Yeo,W-H;Oh,K;Lee,K-H;Kim,M-H;Ryew,S-M;Ahn,S-G;Gao,D;Cangelosi,GA;Chung,J-H
• 通讯作者: Chung,J-H