Histone Modifications Associated w/Senescence in Arabidopsis

拟南芥中与衰老相关的组蛋白修饰

• 批准号: 8270000
• 负责人: JUDY Ann BRUSSLAN
• 金额: $ 14.31万
• 依托单位: CALIFORNIA STATE UNIVERSITY LONG BEACH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8270000
• 关键词: AT-Hook Motifs; Acetylation; Aging; Antibodies; Arabidopsis; Binding; Cell Aging; Cells; ChIP-seq; Chromatin; DNA; DNA Polymerase II; Data; Development; Enzymes; Epigenetic Process; Euchromatin; Eukaryota; Gene Expression; Genes; Genomics; Goals; Harvest; Histones; Human; Lead; Manuscripts; Medical; Mentors; Methylation; Molecular; Mouse-ear Cress; Pilot Projects; Plant Leaves; Plants; Population; Proteins; Publishing; RNA; RNA Polymerase II; Regulation; Research; Research Personnel; Rice; Techniques; Time; Tissues; Ubiquitination; Universities; chromatin immunoprecipitation; genome-wide; histone modification; insight; interest; mutant; overexpression; senescence

DESCRIPTION (provided by applicant): The long-term objective of this project is to define the epigenome associated with senescence in Arabidopsis thaliana. Non-senescent and senescent leaf tissue will be harvested, and chromatin immunoprecipitation followed by Solexa/Illumina high throughput sequencing (ChIP-SEQ) will be used to define DNA regions that bind to modified histones on a genome-wide scale. Gene expression will also be evaluated on a genome-wide scale by ChIP-SEQ using an antibody that recognizes RNA Polymerase II. Histone modifications to be analyzed include methylations, acetylations and ubiquitinations. Cellular aging in humans is delayed by overexpression of histone deacetylases, and a delayed senescence mutant in Arabidopsis, ore7-1D, was recently shown to result from overexpression of an AT-hook chromatin modifying protein. The epigenome of the ore7-1D mutant will be assessed in Specific Aim 4, which may lead to insights into epigenetic regulation of cellular aging that are shared between plants and humans. Another goal of this pilot proposal is to increase the research competitiveness of the PI via an ongoing mentoring relationship with a successful epigenome researcher at a nearby R01 university.

描述（由申请人提供）：该项目的长期目标是定义与拟南芥衰老相关的表观基因组。将收集非阳性和衰老叶组织，并使用染色质免疫沉淀，然后使用Solexa/Illumina高吞吐量测序（CHIP-SEQ）来定义以全基因组量表结合与修饰的组蛋白结合的DNA区域。基因表达还将使用识别RNA聚合酶II的抗体在CHIP-SEQ上以全基因组量表进行评估。要分析的组蛋白修饰包括甲基化，乙酰化和泛素化。人类中的细胞衰老因组蛋白脱乙酰基酶的过表达而延迟，并且最近显示出拟南芥中延迟的衰老突变体是由于对烟素修饰蛋白的过表达而导致的。 ORE7-1D突变体的表观组将在特定的目标4中进行评估，这可能会导致对植物和人类之间的细胞衰老的表观遗传调节的见解。该试点建议的另一个目标是通过与附近R01大学的成功表观基因组研究人员的持续指导关系来提高PI的研究竞争力。