Molecular regulation of diapause in Culex pipiens

淡色库蚊滞育的分子调控

基本信息

  • 批准号:
    8476398
  • 负责人:
  • 金额:
    $ 27.34万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-12-01 至 2015-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Mosquitoes, like most insects, are able to feed and reproduce only a few months each year. The remaining months are spent in a form of dormancy known as diapause. It is the objective of this proposal to understand the molecular mechanisms that regulate this developmental arrest in adults of the northern house mosquito, Culex pipiens, the vector of West Nile virus, with the longterm goal of disrupting this vulnerable phase of the life cycle. The three specific goals of this proposal focus on the molecular pathway that programs the diapauses state. Insulin signaling is a key intermediary in generating the diapause phenotype (traits such as a halt in development, fat accumulation, extended lifespan and increased stress resistance), and this project investigates pathways both up- and downstream of insulin signaling. Our first goal is to identify the pathways leading to the diapause phenotype by working downstream of insulin and FOXO (forkhead transcription factor). Using a FOXO antibody and ChIP sequencing, genes downstream of FOXO will be identified and their putative roles in diapause will be evaluated with functional assays including RNA interference (RNAi). The second goal, upstream of insulin signaling, is to identify the role of clock genes in the transduction of the short day length signals that are used by the mosquito to program entry into diapause. The major clock genes from C. pipiens will be cloned, expression patterns will be evaluated under both long day lengths (nondiapause-inducing) and short day lengths (diapause-inducing), and their function in programming diapause will be evaluated using RNAi. The third aim is to link the photoperiodic response to the downstream insulin/FOXO pathway. After disrupting the clock mechanism we will monitor the impact of these changes on gene expression patterns in the insulin/FOXO pathway. With this information, it will be possible to begin construction of the pathway leading from perception of photoperiod to execution of the diapause phenotype. Any alterations in the diapause response (e.g. diapause prevention, delay in onset or termination, impairment of energy storage or utilization, reduced stress tolerance) could be used as tools for disrupting this critical phase of the mosquito life cycle.
描述(申请人提供):蚊子和大多数昆虫一样,每年只能觅食和繁殖几个月。剩下的几个月是以一种称为滞育的休眠形式度过的。这项建议的目的是了解调控西尼罗河病毒媒介--北部家蚊--淡色库蚊--成虫发育停滞的分子机制,长期目标是扰乱生命周期的这一脆弱阶段。这项提议的三个具体目标集中在编程滞育状态的分子途径上。胰岛素信号是产生滞育表型(如发育停滞、脂肪积累、寿命延长和抗逆性增强等特征)的关键中介,该项目研究了胰岛素信号的上下游途径。我们的第一个目标是通过胰岛素和FOXO(叉头转录因子)下游的工作来确定导致滞育表型的途径。利用FOXO抗体和芯片测序,将识别FOXO下游的基因,并将通过包括RNA干扰(RNAi)在内的功能分析来评估它们在滞育中的可能作用。第二个目标是胰岛素信号的上游,目的是确定时钟基因在传递短日长信号中的作用,这些信号被蚊子用来编程进入滞育。淡色库蚊的主要时钟基因将被克隆,在长日长(非滞育诱导)和短日长(滞育诱导)下的表达模式将被评估,它们在编程滞育中的功能将被用RNAi来评估。第三个目的是将光周期反应与下游的胰岛素/FOXO途径联系起来。在打乱时钟机制后,我们将监测这些变化对胰岛素/FOXO途径中基因表达模式的影响。有了这些信息,就有可能开始构建从感知光周期到执行滞育表型的途径。滞育反应中的任何变化(例如,滞育预防、延迟开始或终止、能量储存或利用受损、胁迫耐受性降低)都可被用作扰乱蚊子生命周期这一关键阶段的工具。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mom Matters: Diapause Characteristics of Culex pipiens-Culex quinquefasciatus (Diptera: Culicidae) Hybrid Mosquitoes.
妈妈很重要:淡色库蚊-致倦库蚊(双翅目:库蚊科)杂交蚊子的滞育特征。
  • DOI:
    10.1093/jme/tju016
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    Meuti,MeganE;Short,ClancyA;Denlinger,DavidL
  • 通讯作者:
    Denlinger,DavidL
Insulin signaling and the regulation of insect diapause.
胰岛素信号传导和昆虫滞育的调节。
  • DOI:
    10.3389/fphys.2013.00189
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    4
  • 作者:
    Sim C;Denlinger DL
  • 通讯作者:
    Denlinger DL
Suppression of allatotropin simulates reproductive diapause in the mosquito Culex pipiens.
抑制尿素可模拟淡色库蚊的生殖滞育。
  • DOI:
    10.1016/j.jinsphys.2014.03.005
  • 发表时间:
    2014-05
  • 期刊:
  • 影响因子:
    2.2
  • 作者:
    Kang DS;Denlinger DL;Sim C
  • 通讯作者:
    Sim C
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DAVID L DENLINGER其他文献

DAVID L DENLINGER的其他文献

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{{ truncateString('DAVID L DENLINGER', 18)}}的其他基金

Molecular regulation of diapause in Culex pipiens
淡色库蚊滞育的分子调控
  • 批准号:
    7217950
  • 财政年份:
    2005
  • 资助金额:
    $ 27.34万
  • 项目类别:
Molecular regulation of diapause in Culex pipiens
淡色库蚊滞育的分子调控
  • 批准号:
    6969645
  • 财政年份:
    2005
  • 资助金额:
    $ 27.34万
  • 项目类别:
Molecular regulation of diapause in Culex pipiens
淡色库蚊滞育的分子调控
  • 批准号:
    7089099
  • 财政年份:
    2005
  • 资助金额:
    $ 27.34万
  • 项目类别:
Molecular regulation of diapause in Culex pipiens
淡色库蚊滞育的分子调控
  • 批准号:
    7387334
  • 财政年份:
    2005
  • 资助金额:
    $ 27.34万
  • 项目类别:
Molecular regulation of diapause in Culex pipiens
淡色库蚊滞育的分子调控
  • 批准号:
    7585282
  • 财政年份:
    2005
  • 资助金额:
    $ 27.34万
  • 项目类别:
REGULATION OF METAMORPHOSIS BEHAVIOR IN THE TSETSE FLY
采采蝇变态行为的调控
  • 批准号:
    3139882
  • 财政年份:
    1989
  • 资助金额:
    $ 27.34万
  • 项目类别:
REGULATION OF METAMORPHOSIS BEHAVIOR IN THE TSETSE FLY
采采蝇变态行为的调控
  • 批准号:
    3139879
  • 财政年份:
    1989
  • 资助金额:
    $ 27.34万
  • 项目类别:
REGULATION OF METAMORPHOSIS BEHAVIOR IN THE TSETSE FLY
采采蝇变态行为的调控
  • 批准号:
    2063270
  • 财政年份:
    1989
  • 资助金额:
    $ 27.34万
  • 项目类别:
REGULATION OF METAMORPHOSIS BEHAVIOR IN THE TSETSE FLY
采采蝇变态行为的调控
  • 批准号:
    2063269
  • 财政年份:
    1989
  • 资助金额:
    $ 27.34万
  • 项目类别:
REGULATION OF METAMORPHOSIS BEHAVIOR IN THE TSETSE FLY
采采蝇变态行为的调控
  • 批准号:
    2063271
  • 财政年份:
    1989
  • 资助金额:
    $ 27.34万
  • 项目类别:

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