Recruitment and activation of DnaB helicase in Escherichia coli DNA replication

大肠杆菌 DNA 复制中 DnaB 解旋酶的招募和激活

• 批准号: 8328635
• 负责人: JON M KAGUNI
• 金额: $ 25.81万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-05 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8328635
• 关键词: Address; Bacteria; Binding; Biochemical; Biological; Cell Cycle; Chromosomes; Complex; DNA; DNA Primase; DNA biosynthesis; DnaB helicase; Escherichia coli; Eukaryota; Event; Genetic; Genome; Lead; Life; Methods; Molecular; Molecular Machines; Movement; Names; Organism; Process; Protein Family; Proteins; Protomer; Replication Initiation; Replication Origin; Research; Single-Stranded DNA; Staging; Testing; helicase; human disease; insight; novel; pathogen; research study

DESCRIPTION (provided by applicant): All cellular organisms rely on a multi-protein molecular machine named the replisome to duplicate their genomes. At replication origins, assembly of replisomes for chromosomal DNA replication is a highly regulated event that is coordinated with the cell cycle. In Escherichia coli, DnaA protein initiates chromosomal DNA replication by orchestrating a step-wise process that leads to two DNA helicases bound to each parental DNA strand, which is necessary for bidirectional fork movement from the chromosomal origin. Other components of the replisome then assemble at the newly formed replication fork. Duplication of the genome follows. The recruitment of the replicative helicase to the chromosomal origin is a critical step during the initiation stage of DNA replication. The long-term objective of this research is to understand the molecular mechanism of recruitment and activation of DnaB, the replicative helicase of Escherichia coli, via genetic, biochemical and molecular biological methods. Because all free-living organisms use a similar mechanism of initiation, these studies should provide insight into how this event occurs in higher organisms.

描述(申请人提供)：所有细胞生物依靠一种名为复制体的多蛋白质分子机器来复制它们的基因组。在复制起点，染色体DNA复制的复制体组装是一个高度受调控的事件，与细胞周期相协调。在大肠杆菌中，Dna A蛋白通过协调一个逐步的过程启动染色体DNA复制，该过程导致两个DNA解旋酶与每个亲本DNA链结合，这是从染色体起源进行双向分叉运动所必需的。然后，复制体的其他组件在新形成的复制叉处组装。随后是基因组的复制。在DNA复制的起始阶段，复制型解旋酶的募集是染色体起源的关键步骤。本研究的长期目标是通过遗传学、生物化学和分子生物学方法，了解大肠杆菌复制解旋酶DNAB募集和激活的分子机制。由于所有自由生活的有机体都使用类似的启动机制，这些研究应该能提供对这一事件如何在高等有机体中发生的洞察。