Laser Spinning Disc Confocal System for live-cell and live-organism microscopy

用于活细胞和活有机体显微镜的激光转盘共焦系统

• 批准号: 8243974
• 负责人: Martha C Soto
• 金额: $ 38.6万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8243974
• 关键词: Actins; Address; Aging; Animal Model; Beginning of Life; Biological; Biological Models; Biological Process; Caenorhabditis elegans; Cell physiology; Cells; Cellular biology; Cilia; Cytoskeleton; Data; Development; Disease; Drosophila genus; Embryo; Embryonic Development; Endocytosis; Event; Extracellular Matrix; Fertility; Fertilization; Funding; Germ Cells; Grant; Growth and Development function; Health; Heart; Homeostasis; Human; Image; Institution; Lasers; Life; Longevity; Manuscripts; Meiosis; Microscope; Microscopy; Morphogenesis; Natural regeneration; Nerve Degeneration; Neurons; New Jersey; Oocytes; Organism; Process; Proteins; Recovery; Regulation; Request for Applications; Research; Research Personnel; Research Project Grants; Resource Sharing; Role; Schools; System; Tissues; United States National Institutes of Health; Universities; Wood material; Work; axonal guidance; base; ciliopathy; data acquisition; egg; egg surface sperm receptor; improved; injured; instrument; interest; medical schools; migration; nervous system development; protein transport; receptor; trafficking

DESCRIPTION (provided by applicant): This application requests funds to purchase a new laser spinning disc microscope (LSDM) to be used as a shared resource for researchers at Rutgers University and Robert Wood Johnson Medical School (RWJMS), two schools on a shared campus in Piscataway, New Jersey. There is currently no laser spinning disc microscope (LSDM) available anywhere on the Rutgers or RWJMS campuses. The NIH-funded researchers requesting this instrument are studying fundamental problems in cell biology, taking advantage of model organisms to study conserved biological processes that are essential for healthy growth and development and that are compromised in disease conditions. C. elegans studies of longevity and neurodegeneration in the Driscoll lab are identifying the processes and the molecules that control lifespan and that become altered in injured neurons. The Wadsworth lab has identified conserved molecules required for proper axonal migrations across metazoan species. Studies of endocytosis in the Grant lab have identified key mechanisms for the regulation of receptor trafficking, identifying many new regulators in worms and humans. C. elegans studies of the regulation of the actin cytoskeleton by the Extracellular Matrix during embryonic development in the Soto Lab are identifying new roles for axonal guidance molecules organizing tissues, and identifying new components of the actin polarization machinery. The Barr Lab uses cilia in developing C. elegans as a model system to study human ciliopathies, or diseases of cilia. Drosophila studies of meiosis in the McKim lab have identified molecules required for proper fertility through the development of germ cells, while C. elegans studies in the Singson lab have identified the first sperm receptor and other molecules required for fertilization and the egg-to-oocyte transition. Live imaging of biological events is required to analyze these cellular processes, yet all of these researchers lack access to a local laser spinning disc confocal microscope. Having this instrument available would have an immediate impact: during a one-month demonstration of the Zeiss LSDM system three manuscripts incorporated images obtained from the demonstration instrument, and the instrument was in constant use. This instrument will clearly accelerate the acquisition of data by the 7 NIH-funded researchers listed in this proposal. The data obtained will dramatically enhance our understanding of such essential cellular phenomena as protein trafficking, polarization of the cytoskeleton and regeneration in neurons. PUBLIC HEALTH RELEVANCE: The addition of a laser spinning disc confocal microscopy system for the researchers at two adjacent universities on a shared campus, Rutgers University and Robert Wood Johnson Medical School, will allow these scientists to pursue research projects that will provide us with the insights necessary to understand how cells behave during normal development and in disease states. These researchers study essential processes of cells including how cells move, how cells like neurons are healed after injury, and how molecules are localized correctly to specific regions of cells.

描述（由申请人提供）：本申请要求资金购买新的激光旋转盘显微镜（LSDM），可作为Rutgers University和Robert Wood Johnson医学院（RWJMS）的研究人员共享资源，这是新泽西州Piscataway共享校园的两所学校。目前，Rutgers或RWJMS校园的任何地方都没有激光旋转盘显微镜（LSDM）。要求该工具的NIH资助的研究人员正在研究细胞生物学中的基本问题，利用模型生物来研究对健康的生长和发展至关重要的保守生物学过程，这些过程在疾病条件下受到损害。 C.秀丽隐杆线虫对Driscoll实验室中寿命和神经退行性的研究正在识别控制寿命并在受伤的神经元中改变的过程和分子。沃兹沃思实验室已经确定了跨后生动物的轴突迁移所需的保守分子。赠款实验室中内吞作用的研究确定了调节受体贩运的关键机制，从而确定了许多蠕虫和人类的新调节剂。秀丽隐杆线虫研究在SOTO实验室中胚胎发育过程中细胞外基质对肌动蛋白细胞骨架的调节正在鉴定组织轴突引导分子组织组织的新作用，并鉴定了肌动蛋白极化机械的新成分。 Barr Lab在开发秀丽隐杆线虫中使用纤毛作为模型系统，研究人类纤毛病或纤毛疾病。麦基姆实验室中减数分裂的果蝇研究已经确定了通过生殖细胞发展适当生育所需的分子，而秀森实验室中的秀丽隐杆线虫研究已经鉴定出了第一个精子受体和其他分子进行肥料和卵 - 卵 - 到卵 - 卵母细胞过渡所需的其他分子。需要对生物事件的实时成像来分析这些细胞过程，但是所有这些研究人员都无法进入局部激光旋转盘共聚焦显微镜。拥有该仪器将有直接的影响：在Zeiss LSDM系统的一个月演示中，三个手稿合并了从演示仪器获得的图像，并且该仪器一直在使用。该工具将清楚地加速该提案中列出的NIH资助的7个资助的研究人员对数据的获取。获得的数据将极大地增强我们对诸如蛋白质运输，细胞骨架极化和神经元再生等必需细胞现象的理解。 公共卫生相关性：在共享校园，罗格斯大学和罗伯特·伍德·约翰逊医学院（Robert Wood Johnson Medical School）上的两个相邻大学的研究人员中添加激光旋转盘共聚焦显微镜系统，将允许这些科学家追求研究项目，这些研究项目将为我们提供所需的见解，以了解在正常发育和疾病状态下细胞在正常发展和疾病状态下如何行事。这些研究人员研究了细胞的基本过程，包括细胞的移动方式，损伤后神经元等细胞的治愈方式以及分子如何正确定位于细胞的特定区域。