Laser Spinning Disc Confocal System for live-cell and live-organism microscopy

用于活细胞和活有机体显微镜的激光转盘共焦系统

• 批准号: 8243974
• 负责人: Martha C Soto
• 金额: $ 38.6万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8243974
• 关键词: Actins; Address; Aging; Animal Model; Beginning of Life; Biological; Biological Models; Biological Process; Caenorhabditis elegans; Cell physiology; Cells; Cellular biology; Cilia; Cytoskeleton; Data; Development; Disease; Drosophila genus; Embryo; Embryonic Development; Endocytosis; Event; Extracellular Matrix; Fertility; Fertilization; Funding; Germ Cells; Grant; Growth and Development function; Health; Heart; Homeostasis; Human; Image; Institution; Lasers; Life; Longevity; Manuscripts; Meiosis; Microscope; Microscopy; Morphogenesis; Natural regeneration; Nerve Degeneration; Neurons; New Jersey; Oocytes; Organism; Process; Proteins; Recovery; Regulation; Request for Applications; Research; Research Personnel; Research Project Grants; Resource Sharing; Role; Schools; System; Tissues; United States National Institutes of Health; Universities; Wood material; Work; axonal guidance; base; ciliopathy; data acquisition; egg; egg surface sperm receptor; improved; injured; instrument; interest; medical schools; migration; nervous system development; protein transport; receptor; trafficking

DESCRIPTION (provided by applicant): This application requests funds to purchase a new laser spinning disc microscope (LSDM) to be used as a shared resource for researchers at Rutgers University and Robert Wood Johnson Medical School (RWJMS), two schools on a shared campus in Piscataway, New Jersey. There is currently no laser spinning disc microscope (LSDM) available anywhere on the Rutgers or RWJMS campuses. The NIH-funded researchers requesting this instrument are studying fundamental problems in cell biology, taking advantage of model organisms to study conserved biological processes that are essential for healthy growth and development and that are compromised in disease conditions. C. elegans studies of longevity and neurodegeneration in the Driscoll lab are identifying the processes and the molecules that control lifespan and that become altered in injured neurons. The Wadsworth lab has identified conserved molecules required for proper axonal migrations across metazoan species. Studies of endocytosis in the Grant lab have identified key mechanisms for the regulation of receptor trafficking, identifying many new regulators in worms and humans. C. elegans studies of the regulation of the actin cytoskeleton by the Extracellular Matrix during embryonic development in the Soto Lab are identifying new roles for axonal guidance molecules organizing tissues, and identifying new components of the actin polarization machinery. The Barr Lab uses cilia in developing C. elegans as a model system to study human ciliopathies, or diseases of cilia. Drosophila studies of meiosis in the McKim lab have identified molecules required for proper fertility through the development of germ cells, while C. elegans studies in the Singson lab have identified the first sperm receptor and other molecules required for fertilization and the egg-to-oocyte transition. Live imaging of biological events is required to analyze these cellular processes, yet all of these researchers lack access to a local laser spinning disc confocal microscope. Having this instrument available would have an immediate impact: during a one-month demonstration of the Zeiss LSDM system three manuscripts incorporated images obtained from the demonstration instrument, and the instrument was in constant use. This instrument will clearly accelerate the acquisition of data by the 7 NIH-funded researchers listed in this proposal. The data obtained will dramatically enhance our understanding of such essential cellular phenomena as protein trafficking, polarization of the cytoskeleton and regeneration in neurons. PUBLIC HEALTH RELEVANCE: The addition of a laser spinning disc confocal microscopy system for the researchers at two adjacent universities on a shared campus, Rutgers University and Robert Wood Johnson Medical School, will allow these scientists to pursue research projects that will provide us with the insights necessary to understand how cells behave during normal development and in disease states. These researchers study essential processes of cells including how cells move, how cells like neurons are healed after injury, and how molecules are localized correctly to specific regions of cells.

描述（由申请人提供）：本申请要求资金购买一台新的激光旋转圆盘显微镜（LSDM），作为罗格斯大学和罗伯特伍德约翰逊医学院（RWJMS）研究人员的共享资源，这两所学校位于新泽西的皮斯卡特维共享校园。目前在罗格斯大学或RWJMS校园的任何地方都没有激光旋转圆盘显微镜（LSDM）。NIH资助的研究人员正在研究细胞生物学中的基本问题，利用模式生物来研究保守的生物过程，这些生物过程对健康的生长和发育至关重要，并且在疾病条件下受到损害。C.在Drivel实验室里，elegans对长寿和神经退化的研究正在确定控制寿命的过程和分子，以及在受伤的神经元中发生改变的过程和分子。沃兹沃斯实验室已经确定了在后生动物物种中进行适当轴突迁移所需的保守分子。格兰特实验室的内吞研究已经确定了调节受体运输的关键机制，确定了蠕虫和人类中的许多新调节剂。C. Elegans在Soto实验室对胚胎发育过程中细胞外基质对肌动蛋白细胞骨架的调节的研究正在确定组织组织的轴突导向分子的新作用，并确定肌动蛋白极化机制的新成分。巴尔实验室利用纤毛来开发C.线虫作为研究人类纤毛病或纤毛疾病的模型系统。麦金实验室对果蝇减数分裂的研究已经确定了通过生殖细胞发育实现适当生育所需的分子，而C。Singson实验室的线虫研究已经确定了第一个精子受体和其他受精和卵母细胞转化所需的分子。分析这些细胞过程需要对生物事件进行实时成像，但所有这些研究人员都无法使用本地激光旋转圆盘共聚焦显微镜。这台仪器的出现将产生立竿见影的效果：在为期一个月的蔡司LSDM系统演示期间，三份手稿包含了从演示仪器获得的图像，该仪器一直在使用。该仪器将明显加快本提案中列出的7名NIH资助的研究人员的数据获取。所获得的数据将极大地增强我们对蛋白质运输、细胞骨架极化和神经元再生等基本细胞现象的理解。 公共卫生相关性：为罗格斯大学和罗伯特伍德约翰逊医学院这两所相邻大学的研究人员增加一个激光旋转圆盘共聚焦显微镜系统，将使这些科学家能够从事研究项目，为我们提供必要的见解，以了解细胞在正常发育和疾病状态下的行为。这些研究人员研究细胞的基本过程，包括细胞如何移动，神经元等细胞如何在损伤后愈合，以及分子如何正确定位到细胞的特定区域。