权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

MicroRNAs in Advanced Prostate Cancer: A miR-21 model

晚期前列腺癌中的 MicroRNA：miR-21 模型

基本信息

批准号：
8463407
负责人：
SHAWN LUPOLD
金额：
$ 31.03万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8463407
关键词：
Accounting Address Advanced Development American Androgen Receptor Apoptotic Applications Grants Area Biological Assay Castration Communities Complementary DNA Diagnosis Disease Disease Outcome Distant Metastasis Employee Strikes Fluorescent in Situ Hybridization Future Gene Dosage Gene Expression Gene Expression Regulation Genes Goals Growth Health Human Immunohistochemistry In Situ Hybridization Knowledge Light Luciferases Malignant Neoplasms Malignant neoplasm of prostate Maps Mediating MicroRNAs Modeling Neoplasm Metastasis Oncogenes Oncogenic PC3 cell line Pathway interactions Patients Post-Transcriptional Regulation Primer Extension Prognostic Marker Promoter Regions Prostate Radical Prostatectomy Recurrence Regulation Regulator Genes Reporter Research Resistance Role Series Signal Pathway Small Interfering RNA Specimen Systemic Therapy Terminal Disease Testing Tissue Microarray Tissues Transcript United States Western Blotting advanced disease castration resistant prostate cancer human tissue innovation male men novel outcome forecast promoter prostate cancer model public health relevance tumor tumor growth tumor progression

项目摘要

DESCRIPTION (provided by applicant): Advanced prostate cancer is an incurable and terminal disease which is characterized by metastasis and castration resistance. The mechanisms of progression to this advanced state are largely unknown. Our long term goal is to identify and characterize pathways which cause this lethal form of prostate cancer. We believe that novel discoveries in this focused area will have significantly impact on the prognosis and treatment of prostate cancer. MicroRNAs are a new class of regulatory molecules which control cellular pathways through post-transcriptional mechanisms. We have identified miR-21 as an Androgen-Receptor-regulated and oncogenic microRNA which is elevated in human prostate cancer. Importantly, miR-21 is sufficient to drive castration resistant tumor growth. In light of these discoveries, and the existing knowledge of miR-21 in other malignancies, we hypothesize that the miR-21 gene locus contributes to the development of advanced prostate cancer. The overall objective of this proposal are to (i) characterize the mechanisms of elevated miR-21 gene expression in human prostate cancer, (ii) to elucidate the pathways utilized by the miR-21 gene locus to promote cancer progression, and to compare miR-21 gene copy number and expression between human prostate cancers which have either been cured by primary therapy or recurred, progressed to metastasis, or castration resistance.

描述（由申请人提供）：晚期前列腺癌是一种无法治愈的晚期疾病，其特征是转移和去势抵抗。进展到这种晚期状态的机制在很大程度上是未知的。我们的长期目标是确定和表征导致这种致命形式的前列腺癌的途径。我们相信，在这一重点领域的新发现将对前列腺癌的预后和治疗产生重大影响。MicroRNA是一类通过转录后机制控制细胞通路的新型调控分子。我们已经确定miR-21是一种雄激素受体调节的致癌microRNA，在人前列腺癌中升高。重要的是，miR-21足以驱动去势抵抗性肿瘤生长。根据这些发现，以及miR-21在其他恶性肿瘤中的现有知识，我们假设miR-21基因位点有助于晚期前列腺癌的发展。本提案的总体目标是（i）表征人前列腺癌中miR-21基因表达升高的机制，（ii）阐明miR-21基因座用于促进癌症进展的途径，并比较通过初级治疗治愈或复发、进展为转移的人前列腺癌之间的miR-21基因拷贝数和表达，或去势抵抗。