Cell Culture and Virus Vector Core

使命：细胞和病毒载体核心实验室(CWCL)的使命是支持高质量 研究并使CCC调查人员能够最大限度地提高工作效率并保持校外竞争力 资金问题。 资产：这一共享资源有三个主要资产，这使得它对 综合癌症中心。首先，它提供关于分子、细胞和病毒技术的咨询， 利用主任和高级工作人员的专业知识。这种咨询包括协助调查人员 新程序的设计、实施和优化。第二，提供专业化服务 以及在研究人员的实验室中不易复制的技术。最近的一些例子包括 细胞毒性试验用于评估预期的抗癌药物，改良的病灶形成试验用于揭示 新型癌基因，利用逆转录病毒载体或四环素诱导型表达重组蛋白 载体，并从组织外植体建立人和小鼠原代细胞系。第三，它提供了 获得关键细胞培养和分子生物学试剂的补贴。 用法：几乎所有癌症中心的成员都使用这个实验室，他们的主要关注点是基础 科学研究。自2001年以来，细胞和病毒载体核心实验室的使用每年都在增加， 这反映在2006年至2009年间增加了85%的实验室费用回收上。超过60%的 CWCL 2010年的退款收入来自癌症中心成员的活跃癌症相关资金。 专门的细胞培养服务和现成的试剂节省了大量资金，提高了 我们的调查人员有能力有效地进行研究并支持 综合癌症中心和维克森林大学。 该实验室的未来方向将包括提供两项新服务，细胞系认证，以及 标准化生产复制能力不强的病毒，用于基因转移。这些服务在中提供 对字段中的更改和用户请求的响应。

Mission: The mission of the Cell and Viral Vector Core Laboratory (CWCL) is to support high quality research and enable CCC investigators to maximize productivity and remain competitive for extramural funding. Assets: This shared resource has three main assets which make it essential to the research mission of the Comprehensive Cancer Center. First, it provides consultation on molecular, cellular and viral techniques, leveraging the expertise of the Director and senior staff. Such consultation includes assisting investigators in the design, implementation and optimization of new procedures. Second, it provides specialized services and techniques not easily reproduced in investigators' laboratories. Some recent examples include cytotoxicity assays to evaluate prospective anticancer agents, modified focus formation assays to uncover novel oncogenes, expression of recombinant proteins using retroviral vectors or tetracycline-inducible vectors, and establishment of human and murine primary cell lines from tissue explants. Third, it provides subsidized access to critical cell culture and molecular biology reagents. Usage: The laboratory is used by nearly all of the Cancer Center members whose primary focus is basic science research. Usage of the Cell and Viral Vector Core Laboratory has increased each year since 2001, and is reflected in laboratory charge-backs which increased 85% between 2006 and 2009. Over 60% of the CWCL charge-back income in 2010 came from Cancer Center members with active cancer-related funding. Specialized cell culture services and the ready availability of reagents at a significant savings enhance the ability of our investigators to efficiently conduct research and support the research efforts of the Comprehensive Cancer Center and Wake Forest University. Future directions for the laboratory will include provision of two new services, cell line authentication, and standardized production of replication-incompetent viruses for gene transfer. These services are provided in response to changes in the field, and user requests.