Cell Culture and Virus Vector Core

细胞培养和病毒载体核心

• 批准号: 9000111
• 负责人: ISABELLE M BERQUIN
• 金额: $ 13.37万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9000111
• 关键词: Antineoplastic Agents; Back; Basic Science; Biological Assay; Bioreactors; Cancer Cell Growth; Cancer Center; Cell Culture Techniques; Cell Line; Cells; Charge; Clinical Research; Comprehensive Cancer Center; Comprehensive Cancer Center of Wake Forest University; Consultations; Extramural Activities; Funding; Future; Gene Transfer; Human; Income; Laboratories; Malignant Neoplasms; Mission; Molecular; Molecular Biology; Mus; Oncogenes; Preparation; Price; Procedures; Production; Productivity; Reagent; Recombinant Proteins; Research; Research Personnel; Research Support; Resource Sharing; Retroviral Vector; Savings; Services; Systems Biology; Techniques; Tetracyclines; Tissues; Viral; Viral Vector; Virus; cost effective; cytotoxicity; design; discount; member; novel; programs; prospective; research study; response; vector

Mission: The mission of the Cell and Viral Vector Core Laboratory (CWCL) is to support high quality research and enable CCC investigators to maximize productivity and remain competitive for extramural funding. Assets: This shared resource has three main assets which make it essential to the research mission of the Comprehensive Cancer Center. First, it provides consultation on molecular, cellular and viral techniques, leveraging the expertise of the Director and senior staff. Such consultation includes assisting investigators in the design, implementation and optimization of new procedures. Second, it provides specialized services and techniques not easily reproduced in investigators' laboratories. Some recent examples include cytotoxicity assays to evaluate prospective anticancer agents, modified focus formation assays to uncover novel oncogenes, expression of recombinant proteins using retroviral vectors or tetracycline-inducible vectors, and establishment of human and murine primary cell lines from tissue explants. Third, it provides subsidized access to critical cell culture and molecular biology reagents. Usage: The laboratory is used by nearly all of the Cancer Center members whose primary focus is basic science research. Usage of the Cell and Viral Vector Core Laboratory has increased each year since 2001, and is reflected in laboratory charge-backs which increased 85% between 2006 and 2009. Over 60% of the CWCL charge-back income in 2010 came from Cancer Center members with active cancer-related funding. Specialized cell culture services and the ready availability of reagents at a significant savings enhance the ability of our investigators to efficiently conduct research and support the research efforts of the Comprehensive Cancer Center and Wake Forest University. Future directions for the laboratory will include provision of two new services, cell line authentication, and standardized production of replication-incompetent viruses for gene transfer. These services are provided in response to changes in the field, and user requests.

使命：细胞和病毒载体核心实验室（CWCL）的使命是支持高质量 研究，使CCC调查人员能够最大限度地提高生产力，并保持竞争力，为校外 经费 资产：这一共享资源有三个主要资产，使其成为必不可少的研究使命。 综合癌症中心。首先，它提供分子、细胞和病毒技术方面的咨询， 利用主任和高级工作人员的专门知识。这种咨询包括协助调查人员 新程序的设计、实施和优化。二是提供专业化服务 以及研究人员实验室不易复制的技术。最近的一些例子包括 细胞毒性试验，以评估潜在的抗癌药物，改良的病灶形成试验，以揭示 新的癌基因，使用逆转录病毒载体或四环素诱导的重组蛋白的表达 载体，以及从组织外植体建立人和鼠原代细胞系。三是提供 获得关键细胞培养和分子生物学试剂的补贴。 用途：几乎所有癌症中心的成员都使用该实验室，其主要重点是基础 科学研究自2001年以来，细胞和病毒载体核心实验室的使用率每年都在增加， 这反映在2006年至2009年期间增加了85%的实验室收费上。的60%以上 2010年，CWCL的退款收入来自癌症中心成员，他们积极提供与癌症相关的资金。 专业的细胞培养服务和试剂的现成可用性，大大节省了成本， 我们的研究人员有效地进行研究和支持的研究工作的能力 综合癌症中心和维克森林大学。 该实验室未来的发展方向将包括提供两项新服务，细胞系认证， 用于基因转移的无复制能力病毒的标准化生产。这些服务在 对现场变化和用户要求的响应。