Cell Culture and Virus Vector Core

细胞培养和病毒载体核心

• 批准号: 7529744
• 负责人: ISABELLE M BERQUIN
• 金额: $ 4.07万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-11 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529744
• 关键词: Accounting; Antineoplastic Agents; Back; Biological Assay; Budgets; Cancer Center; Cancer Center Support Grant; Cell Line; Cell Proliferation; Cells; Charge; Chemicals; Cloning; Collaborations; Communities; Comprehensive Cancer Center of Wake Forest University; Cryopreservation; Cultured Cells; Custom; DNA; Development; Doctor of Philosophy; Drug Formulations; Enzyme-Linked Immunosorbent Assay; Excision; Extramural Activities; Freezing; Genomics; Grant; HIV; HIV-1; Hepatitis Viruses; Human Resources; Income; Institution; Laboratories; Laboratory Personnel; Malignant Neoplasms; Malignant neoplasm of brain; Methods; Mission; Mycoplasma; NCI Center for Cancer Research; Numbers; Oncogenes; Plaque Assay; Preparation; Primary Cell Cultures; Procedures; Production; Productivity; Prostate; Quality Control; RNA purification; Reagent; Recombinant Proteins; Research; Research Peer Review; Research Personnel; Research Project Grants; Retroviral Vector; Savings; Services; Simian virus 5; Speed; Technology; Testing; Tetracycline; Tetracyclines; Training; Transfection; Vaccinia virus; Viral; Virus; Work; cancer therapy; cytotoxicity; member; novel; prospective; repository; research study; tissue culture; tissue/cell culture; tool; vector; virus culture

The mission of the Cell and Virus Vector Core Laboratory is to provide cell culture-related reagents and services that meet the needs of the Cancer Center research community and support high-quality research. The CWCL stocks media and reagents and prepares custom media formulations under strict quality-control procedures, at considerable savings for Cancer Center members. Other functions of the CCWL are to maintain a repository of frozen cell lines, virus stocks and virus vectors, to establish primary cell cultures, to expand tissue culture cells for large-scale experiments, and to perform mycoplasma assays. The CWCL also serves the scientific needs of the Cancer Center researchers by offering customized assistance for research projects. Since its inception in 1978, the CWCL has worked in close collaboration with investigators to develop tools and methods essential for their research. Some recent examples include methods to produce vaccinia virus and simian virus 5 that are used for experimental prostate and brain cancer treatments by CCCWFU investigators. In addition, ELISA tests and plaque assays to evaluate novel compounds for anti-hepatitis virus and HIV-1 activity, cytotoxicity assays to evaluate prospective anticancer agents, modified focus formation assays to uncover novel oncogenes, and the expression of recombinant proteins using retroviral vectors or tetracycline-inducible vectors have been developed. The ready availability of reagents and cells combined with the expertise of the CVVCL staff greatly speeds up the development and implementation of new research projects and reduces the need to hire and train new personnel. Thus, the CWCL is an essential component of the Cancer Center that maximizes the productivity of investigators and enables them to stay competitive for obtaining extramural grant support. Usage of the Cell and Virus Vector Core Laboratory has increased by -20% yearly since 2001 without changes in personnel number. Over 60% of the CVVCL charge-back income in fiscal year 2005 came from Cancer Center members with active peer-reviewed research on approved cancer-related projects. Currently, user charge backs account for -78% of the core's total operating budget, and the CCSG and our Institution both support -11% of the total operating budget. To meet the growing needs of the Cancer Center investigators, we anticipate it will become necessary to hire additional personnel by year 2007.

细胞与病毒载体核心实验室的使命是提供细胞培养相关试剂和 满足癌症中心研究界的需求并支持高质量研究的服务。 CWCL 储备培养基和试剂，并在严格的质量控制下制备定制培养基配方 程序，为癌症中心会员节省大量费用。 CCWL 的其他职能是 维持冷冻细胞系、病毒储液和病毒载体的储存库，以建立原代细胞培养物， 扩大组织培养细胞进行大规模实验，并进行支原体测定。 CWCL 还通过提供定制帮助来满足癌症中心研究人员的科学需求 研究项目。自 1978 年成立以来，CWCL 一直与 研究人员开发对其研究至关重要的工具和方法。最近的一些例子包括 用于实验性前列腺和大脑的牛痘病毒和猿猴病毒 5 的生产方法 CCCWFU 研究人员进行的癌症治疗。此外，还通过 ELISA 测试和噬菌斑测定来评估新型 用于抗肝炎病毒和 HIV-1 活性的化合物，通过细胞毒性测定来评估前瞻性抗癌作用 剂，改进的病灶形成测定法以发现新的癌基因，以及重组的表达 已经开发出使用逆转录病毒载体或四环素诱导载体的蛋白质。随时可用 试剂和细胞与 CVVCL 员工的专业知识相结合，大大加快了开发速度 和实施新的研究项目，并减少雇用和培训新人员的需要。因此， CWCL 是癌症中心的重要组成部分，可最大限度地提高研究人员的生产力 并使他们能够在获得校外拨款支持方面保持竞争力。 自 2001 年以来，细胞和病毒载体核心实验室的使用量每年增加 -20%，而没有 人员数量的变化。 2005 财年 CVVCL 退款收入的 60% 以上来自 癌症中心成员对已批准的癌症相关项目进行了积极的同行评审研究。现在， 用户退款占核心总运营预算的-78%，CCSG 和我们机构 两者都支持总运营预算的-11%。满足癌症中心日益增长的需求 我们预计到 2007 年将有必要雇用更多人员。