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Biological Methods for Enhancing Wound Healing Properties

增强伤口愈合性能的生物学方法

基本信息

批准号：
8455424
负责人：
Angelika Fath-Goodin
金额：
$ 21.75万
依托单位：
PARATECHS CORP.
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-20 至 2015-09-19
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8455424
关键词：
Affect Amputation Analgesics Anti-Bacterial Agents Antibiotic Resistance Antibiotic Therapy Antibiotics Bacteria Bacterial Infections Behavior Binding Biological Breeding Cessation of life Chemicals Chronic Chronic Disease Containment Debridement Development Devices Diabetes Mellitus Disease Dynorphins Endorphins Engineering Enkephalins Europe Excision Exudate Facility Construction Funding Gene Proteins Genes Genetic Engineering Genome Goals Growth Factor Healed Human Immune system Incidence Individual Infection Injection of therapeutic agent Insecta Kentucky Knowledge Label Larva Liquid substance Lower Extremity Lucilia (blowfly)Maggots Mediating Medical Device Methods Microbial Biofilms Molecular Monitor Movement Mutagenesis Necrosis Opioid Receptor Outcome Patients Peptides Phase Production Property Proteins Renaissance Salivary Glands Salivary Proteins Site Small Business Innovation Research Grant Sterility Technology Testing Therapeutic Tissues Transgenes Transgenic Organisms Treatment Cost Ulcer United States United States National Institutes of Health Universities War Wound Healing commercialization conventional therapy cost cost effective diabetic patient egg erythritol anhydride experience feeding fitness fly green bottle fly healing improved interest limb amputation mutant novel strategies phase 2 study professor promoter public health relevance research study targeted delivery therapeutic transgene therapy development trait transgene expression wound

项目摘要

DESCRIPTION (provided by applicant): Chronic wounds and ulcers are increasing with the growing incidence of diabetes and associated illnesses and have annual costs exceeding $20 billion. These non-healing wounds result in chronic disease, limb amputations and death and are complicated by antibiotic resistant bacteria infection. This proposal seeks to improve and develop new capabilities of a biological treatment of such wounds, maggot debridement therapy (MDT) that is effective but underutilized. Some maggot species have evolved to digest fluids, dead tissues and bacteria in wounds and have been recognized for over 200 years for this improved wound healing behavior. For approximately a century, Lucilia sericata maggots have been produced for MDT. This proposal seeks to create transgenic flies with properties that will support increased use of MDT. The goals of the proposal are 1) to produce flightless flies with limited dispersal capability, 2) to engineer fluorescent flies that are more readily observed in wounds, 3) to engineer flies that facilitate analgesic and therapeutic peptide expression to improve patient comfort and wound healing. These mutants will facilitate fly production and also impede genetically engineered insect escape. The objectives of this proposal will support greater use of MDT thereby reducing treatment costs, improving wound healing and limiting the persistent use of antibiotics for treating chronic wounds.

描述(申请人提供)：随着糖尿病和相关疾病发病率的增加，慢性伤口和溃疡也在增加，每年的费用超过200亿美元。这些无法愈合的伤口会导致慢性病、截肢和死亡，并伴有抗药性细菌感染。这项提议旨在改进和发展一种生物治疗这类伤口的新能力，即有效但未得到充分利用的蝇虫清创疗法(MDT)。一些蝇类已经进化到可以消化伤口中的液体、死亡组织和细菌，200多年来一直被认为是这种改善伤口愈合的行为。大约一个世纪以来，丝光绿蝇蛆一直被用于MDT。这项提议寻求创造转基因果蝇，其特性将支持增加MDT的使用。该提案的目标是1)制造扩散能力有限的不会飞的苍蝇，2)改造更容易在伤口中观察到的荧光苍蝇，3)改造有利于止痛和治疗性多肽表达的苍蝇，以改善患者的舒适度和伤口愈合。这些突变体将促进苍蝇的生产，也会阻碍基因工程昆虫的逃逸。这项提案的目标将支持更多地使用MDT，从而降低治疗成本，改善伤口愈合，并限制在治疗慢性伤口时持续使用抗生素。