Interfacing Droplets with Mass Spectrometry for Single-Cell Analysis
将液滴与质谱连接进行单细胞分析
基本信息
- 批准号:8539034
- 负责人:
- 金额:$ 20.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2015-08-31
- 项目状态:已结题
- 来源:
- 关键词:AccelerationAddressAirAtmospheric PressureBiochemicalBlood capillariesCancer DetectionCell VolumesCellsChemicalsComplexDNADetectionDevelopmentDiseaseGene ExpressionGoalsIon TransportIonsKnowledgeLaboratoriesLipidsLiquid substanceMass Spectrum AnalysisMeasurementMicrofluidicsOperating SystemOptical MethodsOpticsOrganismPeptidesPhysiologyPolymerase Chain ReactionPositioning AttributeProteinsReactionResearchResolutionSamplingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSystemTechniquesTechnologyVacuumWorkanalytical methodbasebiological systemscapillarychemical reactiondesign and constructionexperienceimprovedinstrumentinterestionizationionization techniquemass spectrometermeetingsnext generationoptical trapspressurepublic health relevanceresponsesingle cell analysissingle moleculesmall molecule
项目摘要
This application addresses RFA-GM-1-1009, Exceptional, Unconventional Research Enabling Knowledge Acceleration (EUREKA)(R01). The basic unit of any biological system is the cell, and malfunctions at the single-cell level can result in devastating diseases. To understand the physiology and functions of any biological system, therefore, it is important to achieve measurements with single-cell resolutions. This proposal aims to employ advanced mass spectrometric technologies which, when appropriately combined with new droplet manipulation techniques, will allow us to meet the tremendous challenge of analyzing the contents of single cells with high sensitivty and information content using mass spectrometry. Specifically, the aim of this project is: Design and construct a mass spectrometer with the appropriate interface for ionizing and analyzing the contents of single cells trapped in liquid droplets. The interface will be a droplet nanolab platform with the optical trapping and positioning of droplets in air, which will be used towards achieving the efficient ionization of single-droplet contents and ion transport for mass spectrometry analysis. Atmospheric pressure chemical ionization (APCI) will be used for the analysis of small-molecule (e.g. metabolites and lipids) contents of single cells. MALDI will be used for the analysis of peptide and protein contents of single cells. The cell is the basic unit of any biological system; to understand the functions and malfunctions of an organism will thus necessitate measurements at the single-cell level. Yet the biochemical analysis of single cells is highly challenging, owing to both the minute amounts of sample available as well as the high complexity of the sample. In this proposed project, we aim to tackle these challenges by pushing the limit of sensitivity of mass spectrometry for analyzing the minute but highly concentrated single-cell derived samples present in droplets.
PUBLIC HEALTH RELEVANCE: The cell is the basic unit of any biological system; to understand the functions and malfunctions of an organism will thus necessitate measurements at the single-cell level. Yet the biochemical analysis of single cells is highly challenging, owing to both the minute amounts of sample available as well as the high complexity of the sample. In this proposed project, we aim to tackle these challenges by pushing the limit of sensitivity of mass spectrometry for analyzing the minute but highly concentrated single-cell derived samples present in droplets.
此应用程序针对RFA-GM-1-1009,特殊的、非传统的研究支持知识加速(Eureka)(R01)。任何生物系统的基本单位都是细胞,单细胞水平的故障可能会导致毁灭性的疾病。因此,要了解任何生物系统的生理和功能,实现单细胞分辨率的测量是很重要的。这项建议旨在采用先进的质谱学技术,当这些技术与新的液滴操纵技术适当地结合在一起时,将使我们能够应对利用质谱学分析单个细胞的高灵敏度和信息含量的巨大挑战。具体地说,该项目的目标是:设计和建造一个具有适当接口的质谱仪,用于电离和分析捕获在液滴中的单个细胞的内容物。该界面将是一个液滴NanoLab平台,具有对空气中液滴的光学捕获和定位,将用于实现单个液滴内容物的有效电离和用于质谱分析的离子传输。大气压化学电离(APCI)将用于分析单个细胞的小分子(如代谢物和脂类)含量。MALDI将用于分析单细胞的多肽和蛋白质含量。细胞是任何生物系统的基本单位;因此,要了解生物体的功能和故障,就必须在单细胞水平上进行测量。然而,由于可获得的样本数量很少,而且样本的复杂性很高,单细胞的生化分析具有很大的挑战性。在这个拟议的项目中,我们的目标是通过突破质谱学的灵敏度极限来应对这些挑战,以分析液滴中存在的微小但高度浓缩的单细胞衍生样品。
与公共健康相关:细胞是任何生物系统的基本单位;因此,要了解生物体的功能和故障,就必须在单细胞水平上进行测量。然而,由于可获得的样本数量很少,而且样本的复杂性很高,单细胞的生化分析具有很大的挑战性。在这个拟议的项目中,我们的目标是通过突破质谱学的灵敏度极限来应对这些挑战,以分析液滴中存在的微小但高度浓缩的单细胞衍生样品。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Modulating patterns of two-phase flow with electric fields.
用电场调制两相流模式。
- DOI:10.1063/1.4891099
- 发表时间:2014
- 期刊:
- 影响因子:3.2
- 作者:Liu,Dingsheng;Hakimi,Bejan;Volny,Michael;Rolfs,Joelle;Anand,RobbynK;Turecek,Frantisek;Chiu,DanielT
- 通讯作者:Chiu,DanielT
Nanoliter segmented-flow sampling mass spectrometry with online compartmentalization.
- DOI:10.1021/ac500365r
- 发表时间:2014-04-01
- 期刊:
- 影响因子:7.4
- 作者:Volny, Michael;Rolfs, Joelle;Hakimi, Bejan;Frycak, Petr;Schneider, Thomas;Liu, Dingsheng;Yen, Gloria;Chiu, Daniel T.;Turecek, Frantisek
- 通讯作者:Turecek, Frantisek
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
FRANTISEK TURECEK其他文献
FRANTISEK TURECEK的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('FRANTISEK TURECEK', 18)}}的其他基金
Interfacing Droplets with Mass Spectrometry for Single-Cell Analysis
将液滴与质谱连接进行单细胞分析
- 批准号:
8324269 - 财政年份:2010
- 资助金额:
$ 20.8万 - 项目类别:
Interfacing Droplets with Mass Spectrometry for Single-Cell Analysis
将液滴与质谱连接进行单细胞分析
- 批准号:
8136616 - 财政年份:2010
- 资助金额:
$ 20.8万 - 项目类别:
Interfacing Droplets with Mass Spectrometry for Single-Cell Analysis
将液滴与质谱连接进行单细胞分析
- 批准号:
7993923 - 财政年份:2010
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
6929091 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
7426533 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
6612519 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
7143170 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
6803043 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
7265273 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
Multiplex Analysis of Inborn Errors of Metabolism
先天性代谢缺陷的多重分析
- 批准号:
7487094 - 财政年份:1999
- 资助金额:
$ 20.8万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Standard Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
EU-Funded
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 20.8万 - 项目类别:
Research Grant