Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zo

采用等速电泳法直接分析人体组织中的高氯酸盐和碘化物

• 批准号: 8433122
• 负责人: Vladislav Dolnik
• 金额: $ 25万
• 依托单位: ALCOR BIOSEPARATIONS
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-27 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8433122
• 关键词: Address; Animals; Ballistics; Biological Monitoring; Body Fluids; Chloride Ion; Chlorides; Chromatography; Coupled; Data; Detection; Electrolytes; Electrophoresis; Ensure; Funding Opportunities; Health; Human; Human Milk; Human body; Hypothyroidism; Inorganic Sulfates; Intake; Iodides; Ions; Methods; Monitor; Perchlorates; Reproducibility; Running; Sampling; Serum; System; Thyroid Gland; Tissues; Unspecified or Sulfate Ion Sulfates; analytical method; base; design; drinking water; human tissue; new technology

DESCRIPTION (provided by applicant): Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zone electrophoresis on chip Project Summary This application addresses the Funding Opportunity RFA-ES-12-004 Novel technologies for rapid and sensitive biomonitoring in humans. The aim of this project is to develop and validate a fast, sensitive, and reproducible method for direct analysis of perchlorate and iodide in human body fluids by isotachophoresis-zone electrophoresis on chip. Perchlorate, which is used in the propulsion systems of intercontinental ballistic missiles and the space shuttles, was found in drinking water in western U.S.A. Recent studies repeatedly showed significantly elevated perchlorate levels in breast milk across the entire U.S.A. In terms of health effect, perchlorate competes with iodide for the intake by mammalian thyroid gland and can cause hypothyroidism. Monitoring perchlorate level in human body fluids is therefore of outmost importance. Perchlorate is typically analyzed by ion chromatography after deproteinization in animal and human body fluids and tissues. We propose a fast method for direct analysis on chip where perchlorate and iodide from body fluids will be separated and concentrated by isotachophoresis and detected by zone electrophoresis with capacitively-coupled contactless conductivity detection. The method will quantitate perchlorate and iodide at 1 nM level with the run-to-run reproducibility of 5% within 10 minutes. PUBLIC HEALTH RELEVANCE: Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zone electrophoresis on chip Project Narrative This project will develop and validate a fast, sensitive, and reproducible method for direct analysis of perchlorate and iodide in human body fluids by isotachophoresis-zone electrophoresis on chip. Perchlorate, which is used in the propulsion systems of intercontinental ballistic missiles, has been detected in drinking water in over 30 states. In human body it competes with iodide for intake by thyroid gland and may cause hypothyroidism, particularly at newborns. Perchlorate presence represents a significant health risk and the proposed method, which will allow monitoring both perchlorate and iodide in body fluids, is of utmost importance.

描述（由申请人提供）：通过芯片上的等速荧光区带电泳直接分析人体组织中的高氯酸盐和碘化物项目摘要：本申请涉及资助机会RFA-ES-12-004用于快速和灵敏的人体生物监测的新技术。本项目的目的是开发和验证一种快速、灵敏、可重复的芯片等速电泳直接分析人体体液中高氯酸盐和碘化物的方法。在美国西部的饮用水中发现了用于洲际弹道导弹和航天飞机推进系统的高氯酸盐。最近的研究反复表明，在整个美国，母乳中的高氯酸盐含量显著升高。就健康影响而言，高氯酸盐与碘化物竞争哺乳动物甲状腺的摄入，并可能导致甲状腺功能减退。因此，监测人体体液中的高氯酸盐水平至关重要。高氯酸盐在动物和人体体液和组织中脱蛋白后，通常用离子色谱法进行分析。我们提出了一种快速的芯片直接分析方法，将体液中的高氯酸盐和碘化物用等速电泳分离浓缩，并用电容耦合非接触式电导率检测的区带电泳检测。该方法可在1 nM的水平上定量高氯酸盐和碘化物，10年内的运行重复性为5%