Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zo

描述（由申请人提供）：通过芯片上的等速电泳区带电泳直接分析人体组织中的高氯酸盐和碘化物项目摘要本申请涉及资助机会RFA-ES-12-004用于人类快速灵敏生物监测的新技术。本项目的目的是开发和验证一种快速，灵敏，重复性好的方法，用于直接分析人体体液中的高氯酸盐和碘化物的等速电泳芯片。在美国西部的饮用水中发现了高氯酸盐，用于洲际弹道导弹和航天飞机的推进系统。最近的研究一再表明，整个美国母乳中的高氯酸盐水平显着升高。就健康影响而言，高氯酸盐与碘化物竞争哺乳动物甲状腺的摄入量，并可导致甲状腺功能减退症。因此，监测人体体液中的高氯酸盐水平至关重要。高氯酸盐通常在动物和人体体液和组织中脱蛋白后通过离子色谱法进行分析。我们提出了一种快速的方法，直接分析芯片上的高氯酸盐和碘化物从体液将被分离和等速电泳和检测区带电泳与电容耦合非接触式电导检测器的浓缩。该方法将在1 nM水平下定量高氯酸盐和碘化物，运行间重现性为5%，10 分钟 公共卫生相关性：本项目将开发并验证一种快速、灵敏、重复性好的方法，通过等速电泳芯片直接分析人体体液中的高氯酸盐和碘化物。用于洲际弹道导弹推进系统的高氯酸盐已在30多个州的饮用水中被检测到。在人体内，它与碘竞争甲状腺的摄入，并可能导致甲状腺功能减退，特别是在新生儿中。高氯酸盐的存在代表了一个重大的健康风险和拟议的方法，这将允许监测高氯酸盐和碘化物在体液中，是至关重要的。

DESCRIPTION (provided by applicant): Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zone electrophoresis on chip Project Summary This application addresses the Funding Opportunity RFA-ES-12-004 Novel technologies for rapid and sensitive biomonitoring in humans. The aim of this project is to develop and validate a fast, sensitive, and reproducible method for direct analysis of perchlorate and iodide in human body fluids by isotachophoresis-zone electrophoresis on chip. Perchlorate, which is used in the propulsion systems of intercontinental ballistic missiles and the space shuttles, was found in drinking water in western U.S.A. Recent studies repeatedly showed significantly elevated perchlorate levels in breast milk across the entire U.S.A. In terms of health effect, perchlorate competes with iodide for the intake by mammalian thyroid gland and can cause hypothyroidism. Monitoring perchlorate level in human body fluids is therefore of outmost importance. Perchlorate is typically analyzed by ion chromatography after deproteinization in animal and human body fluids and tissues. We propose a fast method for direct analysis on chip where perchlorate and iodide from body fluids will be separated and concentrated by isotachophoresis and detected by zone electrophoresis with capacitively-coupled contactless conductivity detection. The method will quantitate perchlorate and iodide at 1 nM level with the run-to-run reproducibility of 5% within 10 minutes. PUBLIC HEALTH RELEVANCE: Direct analysis of perchlorate and iodide in human tissues by isotachophoresis-zone electrophoresis on chip Project Narrative This project will develop and validate a fast, sensitive, and reproducible method for direct analysis of perchlorate and iodide in human body fluids by isotachophoresis-zone electrophoresis on chip. Perchlorate, which is used in the propulsion systems of intercontinental ballistic missiles, has been detected in drinking water in over 30 states. In human body it competes with iodide for intake by thyroid gland and may cause hypothyroidism, particularly at newborns. Perchlorate presence represents a significant health risk and the proposed method, which will allow monitoring both perchlorate and iodide in body fluids, is of utmost importance.