Wall coating for microchip capillary electrophoresis

微芯片毛细管电泳壁涂料

• 批准号: 6992241
• 负责人: Vladislav Dolnik
• 金额: $ 16.97万
• 依托单位: ALCOR BIOSEPARATIONS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6992241
• 关键词: DNA; capillary electrophoresis; isoelectric point; microarray technology; nucleic acid sequence; plastics; polymethacrylate; surface coating; surface property; technology /technique development

DESCRIPTION (provided by applicant): This proposal aims at the development of hydrophilic, hydrolytically stable wall coatings for plastic microchips made from poly (methyl methacrylate) (PMMA) and/or polycarbonate (PC). In analytical chemistry of the last decade, miniaturization of analytical operations and their integration on a microchip has become a hot topic. Capillary electrophoresis (CE) has been involved at the forefront of these efforts frequently. However, the miniaturization of the channel size emphasizes the influence of chemical properties of the channel surface. Therefore a negative charge that is inherently present at the surface results in adsorption of analytes on the channel wall and also in electroosmotic flow that is driven by these functionalities and compromises high-resolution CE separations. So far only a few coatings have been applied to glass and plastic microchips. This project will develop a hydrolytically stable, hydrophilic wall coating for plastic microchips made from PMMA and/or PC. The proposed coating will not carry any charge and will thus eliminate electroosmotic flow and minimize adsorption of analytes on the channel wall. As the top layer it will contain either natural polysaccharides locust bean gum or guaran, or a synthetic polymer poly (acryloyl diethanolamine) attached by thermal immobilization. We will characterize this coating by measuring its electroosmotic mobility JIEEO (< 0.5 * 10-9 m2V-1s-1) and stability after a treatment with NaOH solutions. We will demonstrate the utility of this coating by isoelectric focusing of model proteins and by CE of M13mp18 DMA sequencing fragments.

描述(由申请人提供)：本建议旨在开发用于由聚甲基丙烯酸甲酯(PMMA)和/或聚碳酸酯(PC)制成的塑料微芯片的亲水性、水解性稳定的壁面涂料。在过去十年的分析化学中，分析操作的小型化及其在微芯片上的集成已经成为一个热门话题。毛细管电泳法(CE)经常处于这些努力的前沿。然而，通道尺寸的小型化强调了通道表面化学性质的影响。因此，表面固有的负电荷会导致分析物在通道壁上的吸附，也会导致由这些功能驱动的电渗流，从而影响高分辨率的CE分离。到目前为止，只有几种涂层被应用于玻璃和塑料微芯片。该项目将开发一种用于由PMMA和/或PC制成的塑料微芯片的水解性稳定的亲水性墙面涂料。建议的涂层将不携带任何电荷，从而消除电渗流，并最大限度地减少分析物在通道壁上的吸附。作为顶层，它将包含天然多糖刺槐豆胶或瓜拉胶，或通过热固定连接的合成聚合物聚(丙烯酰二乙醇胺)。我们将通过测量涂层的电渗透迁移率JIEEO(&lt；0.5*10-9m2V-1s-1)和用NaOH溶液处理后的稳定性来表征该涂层。我们将通过模型蛋白的等电聚焦和M13mp18 DMA测序片段的CE来证明这种涂层的实用性。