Mechanisms of UV-induced skin carcinogenesis

紫外线诱发皮肤癌的机制

• 批准号: 8197882
• 负责人: LAURA A HANSEN
• 金额: $ 29.89万
• 依托单位: CREIGHTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8197882
• 关键词: Ablation; Adenovirus Infections; Affect; American; Apoptosis; Biological; Biological Assay; Biological Process; Biotechnology; Blinded; Blood capillaries; Cancer Etiology; Carcinogenesis Mechanism; Cell Culture Techniques; Cell Cycle Arrest; Cell Cycle Progression; Cell Cycle Regulation; Cell Line; Cells; Chronic; Coculture Techniques; Conditioned Culture Media; DNA Damage; DNA Repair; DNA damage checkpoint; Data; Development; Dose; Ectopic Expression; Endothelial Cells; Ensure; Epidermal Growth Factor Receptor; Exposure to; Extracellular Matrix; Gene Expression; Genetic; Health; Human; In Vitro; Inflammation; Journals; Length; Malignant - descriptor; Malignant Epithelial Cell; Malignant Neoplasms; Matrix Metalloproteinases; Measures; Microscopic; Modeling; Molecular; Molecular Carcinogenesis; Orphan; PI3K/AKT; Pathology; Phase; Phosphorylation; Principal Investigator; Protease Inhibitor; Proto-Oncogene Proteins c-akt; Proto-Oncogenes; Publications; Receptor Protein-Tyrosine Kinases; Research; Research Personnel; S Phase Arrest; Sampling; Signal Transduction; Skin; Skin Cancer; Skin Carcinogenesis; Skin Carcinoma; Skin Neoplasms; Solid; Staging; Sunlight; System; Text; Therapeutic Intervention; Thrombospondin 1; Time; Tube; Tumor Cell Invasion; UV induced; Ultraviolet Rays; United States; United States National Institutes of Health; Up-Regulation; Work; angiogenesis; cancer diagnosis; capillary; experience; keratinocyte; migration; novel; programs; research study; response; skin cancer prevention; therapy design; tumor; tumor progression; ultraviolet; ultraviolet irradiation

DESCRIPTION (provided by applicant): Exposure to the ultraviolet (UV) component of sunlight is the major cause of nonmelanoma skin cancer, the most common form of cancer in the United States. Despite its significance for human health, however, the mechanisms through which UV causes cancer are not well understood. One potential mechanism for skin carcinogenesis involves the UV-induced activation of Erbb2, a proto-oncogene and orphan receptor tyrosine kinase. Our preliminary data reveal novel mechanisms through which Erbb2 contributes to skin carcinogenesis at multiple stages, both during tumor development and progression. Our central hypotheses are 1) that Erbb2 promotes cell cycle progression and thus increases UV-induced skin carcinogenesis by dampening DNA damage response checkpoints and 2) that Erbb2's suppression of proteinase inhibitor Thrombospondin 1 and its upregulation of matrix metalloproteinases stimulate keratinocyte invasion and angiogenesis, leading to malignant progression. The long-term objective of this application is to understand the biological functions of Erbb2 during carcinogenesis and the mechanisms through which Erbb2 acts. Studies are proposed to reveal the biological significance of Erbb2 in skin tumor development and progression, to determine the molecular mechanisms by which Erbb2 regulates cell cycle progression and a DNA damage checkpoint through Cdc25a, and to determine how Erbb2 causes skin cancer progression. These studies will use models that we have developed for both the inhibition and genetic ablation of Erbb2 in the skin. Upon completion, these studies will reveal the basic mechanisms of cell cycle regulation and checkpoint control following UV exposure as well as novel mechanisms through which Erbb2 regulates cell cycle arrest after DNA damage and promotes malignant progression. This research will provide a comprehensive analysis of the importance of Erbb2 in skin carcinogenesis, will elucidate novel mechanisms through which Erbb2 acts, and will provide new targets for therapeutic intervention during skin carcinogenesis. Ultraviolet irradiation is the cause of most of the more than one million skin cancers diagnosed in the United States each year. The proposed research will reveal basic molecular mechanisms responsible for the induction of skin cancer in response to ultraviolet irradiation. Successful completion of the proposed research will allow for the development of novel therapies designed to treat or prevent skin cancer.

描述（由申请人提供）：暴露于阳光的紫外线（UV）成分是非黑色素瘤皮肤癌的主要原因，非黑色素瘤皮肤癌是美国最常见的癌症形式。尽管它对人类健康的重要性，但是，紫外线导致癌症的机制还没有得到很好的理解。皮肤癌发生的一个潜在机制涉及Erbb2（一种原癌基因和孤儿受体酪氨酸激酶）的UV诱导活化。我们的初步数据揭示了Erbb2在肿瘤发展和进展的多个阶段促进皮肤癌发生的新机制。我们的中心假设是1）Erbb2促进细胞周期进展，从而通过抑制DNA损伤反应检查点而增加UV诱导的皮肤癌发生，以及2）Erbb2对蛋白酶抑制剂血小板反应蛋白1的抑制及其对基质金属蛋白酶的上调刺激角质形成细胞侵袭和血管生成，导致恶性进展。本申请的长期目标是了解Erbb2在致癌过程中的生物学功能以及Erbb2的作用机制。本研究旨在揭示Erbb2在皮肤肿瘤发生和发展中的生物学意义，确定Erbb2通过Cdc25a调节细胞周期进程和DNA损伤检查点的分子机制，并确定Erbb2如何导致皮肤癌进展。这些研究将使用我们开发的用于抑制和基因消融皮肤中Erbb2的模型。完成后，这些研究将揭示紫外线暴露后细胞周期调控和检查点控制的基本机制，以及Erbb2在DNA损伤后调节细胞周期阻滞并促进恶性进展的新机制。这项研究将全面分析Erbb2在皮肤癌发生中的重要性，阐明Erbb2作用的新机制，并为皮肤癌发生过程中的治疗干预提供新靶点。 紫外线照射是美国每年诊断出的100多万皮肤癌中的大多数的原因。这项拟议中的研究将揭示紫外线照射诱发皮肤癌的基本分子机制。成功完成拟议的研究将允许开发旨在治疗或预防皮肤癌的新疗法。

项目成果

Mouse skin models for carcinogenic hazard identification: utilities and challenges.

用于致癌危害识别的小鼠皮肤模型：实用性和挑战。

• DOI: 10.1080/01926230701748131
• 发表时间: 2007
• 期刊: Toxicologic pathology
• 影响因子: 1.5
• 作者: Lynch,Dave;Svoboda,Jessica;Putta,Sumanth;Hofland,HansEJ;Chern,WendyH;Hansen,LauraA
• 通讯作者: Hansen,LauraA

The human promyelocytic leukemia protein is a tumor suppressor for murine skin carcinogenesis.

• DOI: 10.1002/mc.20498
• 发表时间: 2009-07
• 期刊: MOLECULAR CARCINOGENESIS
• 影响因子: 4.6
• 作者: Virador, Victoria M.;Flores-Obando, Rafael E.;Berry, Adam;Patel, Rinal;Zakhari, Julia;Lo, Yu-Chien;Strain, Kathryn;Anders, Joanna;Cataisson, Christophe;Hansen, Laura A.;Yuspa, Stuart H.
• 通讯作者: Yuspa, Stuart H.