DEVELOPMENT OF AN INTELLIGENT SAMPLE INTRODUCTION SYSTEM FOR MASS SPECTROMETRY

质谱智能进样系统的开发

• 批准号: 8590085
• 负责人: Justin Michael Wiseman
• 金额: $ 35万
• 依托单位: PROSOLIA, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2014-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590085
• 关键词: Adoption; Affect; Area; Aspirate substance; Automation; Bacteria; Biological; Biological Markers; Blood capillaries; Classification; Computer software; Data; Detection; Development; Devices; Diagnostic; Digestion; Electrospray Ionization; Ensure; Fingerprint; Gases; Government; Growth; Hand; Height; Histologic; Image; In Situ; Investigation; Ions; Laboratories; Lasers; Libraries; Licensing; Life; Liquid substance; Literature; Maintenance; Marketing; Mass Spectrum Analysis; Measurement; Methodology; Methods; Metric; Microfluidics; Modeling; Modification; Organism; Performance; Phase; Plant Roots; Positioning Attribute; Process; Proteomics; Pump; Relative (related person); Reproducibility; Research; Research Personnel; Resolution; Sample Size; Sampling; Solid; Solutions; Solvents; Spottings; Stress; Surface; System; Systems Development; Techniques; Technology; Time; Tissues; United States National Institutes of Health; Variant; Vision; Work; base; capillary; data integration; in vivo; innovation; instrument; interest; microorganism; new technology; operation; programs; prototype; public health relevance; research study; sensor; user-friendly

DESCRIPTION: Development of an Intelligent Sample Introduction System for Mass Spectrometry There is a pressing need for new technologies that enable rapid measurements without significant compromise to the analytical data. Herein, we propose to develop a robust mass spectrometry (MS) based surface sampling device that enables direct analysis of a variety of materials including biological tissue and live microorganisms that promises to deliver on this objective. The basis for this device is the Liquid Micro junction Surface Sampling Probe (LMJSSP), a set of coaxial microfluidic capillaries that are pumped and aspirated to bring solvent to and away from a surface of interest, where micro extraction takes place at the interface between the probe and the sample. Prosolia has licensed the LMJSSP technology from AB Sciex and will partner with the original inventor at Oak Ridge National Laboratory via the NIH Lab to Marketplace program to create an advanced prototype towards a commercially viable product by making the technology more robust and user-friendly for applications in various bio analytical workflows. The tasks of this project can be divided into three Aims. The first and second Aims are to ensure that the hardware of the device is sufficiently easy to use for commercial viability. This includes characterization of the present prototype and exploration of a few promising methods for automatic control of matched liquid flows required for a continuous extractive junction to persist between probe and surface. Additionally, establishing a mechanism for maintaining an ideal distance between the probe and surface will be critical for successful implementation. Upon establishing methods for flow and height management with optimized control and reasonable potential for manufacture, these hardware modifications will be used to determine the analytical conditions for instrument operation. Specifically, standards will be created to determine the profile and efficiency of extraction under a variety of conditions (including within different laboratories). These will determine the expected limits for potential use, maintenance, and support to ensure robust operations calibrated for a variety of methodologies. During this section of work, investigations into potential increases in resolution from the standard probing arrangement via smaller variants will be performed to identify analytical metrics at histologically relevant sampling sizes of interest to diagnostic and biomarker researchers. Early experiments using another MS-based surface sampling technique, Desorption Electrospray Ionization, for direct bio analysis of live microorganisms were able to generate spectral profiles for bacterial colonies; however, the process to generate these results required frequent cleaning and exchanging of instrument capillaries due to fouling with bacteria that were blown off the surface and into the instrument inlet. Preliminary spectra obtained using our prototype LMJSSP system have been collected in a much more facile manner, and have shown some particularly interesting features for distinguishing and characterizing different species and strains of bacteria. However, more fingerprints must be acquired in order to determine statistical significance and applicability of these data. In addition, we will establish he effects of sampling at various growth points and stress conditions in order to generate a potential library for automated classification. After implementing changes for automation and optimization, our prototype for direct analysis of surfaces, will be a simple sample introduction system transitioning from an academic prototype used by a few to a commercial prototype that can be deployed to many. By hewing to the milestones we propose: making appropriate hardware changes, determining analytical metrics corresponding to solvents and probe sizes, and gathering a statistically relevant number of profiles for bacterial analysis, the capacity for hands-off microorganism profiling will have been assessed. As such our phase II proposal will include further hardware refinements, a broader selection of samples, enhanced data integration, and processing software.

目前迫切需要能够在不影响分析数据的情况下进行快速测量的新技术。在此，我们建议开发一种强大的基于质谱（MS）的表面采样设备，该设备可以直接分析各种材料，包括生物组织和活微生物，有望实现这一目标。该装置的基础是液体微结表面采样探头（LMJSSP），这是一组同轴微流体毛细血管，通过泵送和吸气将溶剂带入或离开感兴趣的表面，在探针和样品之间的界面处进行微萃取。Prosolia已经从AB Sciex获得了LMJSSP技术的许可，并将通过NIH实验室与橡树岭国家实验室的原始发明人合作，通过使该技术更加强大和用户友好的应用于各种生物分析工作流程，创建一个商业上可行的产品的先进原型。这个项目的任务可以分为三个目标。第一个和第二个目标是确保设备的硬件足够容易使用