Fluctuations and entropy in the energetics and function of protein complexes

蛋白质复合物的能量学和功能中的波动和熵

基本信息

  • 批准号:
    8515476
  • 负责人:
  • 金额:
    $ 35.04万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-08-01 至 2016-05-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The fundamental premise of this proposal is that proteins are dynamic entities, that their internal motion is an expression of an inherent and significant amount of conformational entropy and that changes in conformation entropy can greatly influence the energetics of protein function. Below we will summarize recent results that support these assertions. Perhaps the simplest functional context is the binding of a ligand by a protein. Thus our general hypothesis is that the thermodynamics of protein-ligand interactions can be influenced via changes in the protein internal entropy. Though this idea has been in the literature for some time it is only recently that the experimental methods that are capable of illuminating it have become available. We will test the generality of a role for protein dynamics (and the entropy it represents) in molecular recognition by proteins and in the more sophisticated allosteric response. The former will build upon an existing array of examples that indicate a general roughly linear relationship between the contributions of a change in conformational entropy upon ligand binding and the overall binding entropy. Though such a relationship is not required its existence suggests that evolution has exploited conformational entropy in the optimization of protein-ligand thermodynamics. This idea will be quantitatively explored using the recently calibrated "entropy meter" that relies on a dynamical proxy for conformational entropy. Comprehensive measurements of internal protein motion will be undertaken using the now well-established solution NMR relaxation methods. In an effort to more fully understand the propagation of dynamics within the protein matrix, a high-pressure perturbation study of protein motion will be carried out to illuminate coupling of motion. Recent work by others on the catabolite activator protein suggests that conformational entropy may play a central role in protein-DNA recognition. We therefore propose to examine the structural and dynamic properties underlying protein-DNA recognition in the lac repressor. The lac repressor is a paradigm for genetic regulation in prokaryotes. Overall these studies will greatly expand our appreciation of the nature of protein motion and the role of the conformational entropy that it represents in the energetics of protein function.
描述(由申请人提供):该提议的基本前提是蛋白质是动态实体,它们的内部运动是固有的和显著量的构象熵的表达,并且构象熵的变化可以极大地影响蛋白质功能的能量学。下面我们将总结支持这些断言的最新结果。也许最简单的功能背景是蛋白质与配体的结合。因此,我们的一般假设是,蛋白质-配体相互作用的热力学可以通过蛋白质内部熵的变化来影响。虽然这个想法已经在文献中有一段时间了,但直到最近才有实验方法能够阐明它。我们将测试蛋白质动力学(和它所代表的熵)在蛋白质分子识别和更复杂的变构反应中的作用的一般性。前者将建立在现有的一系列的例子,表明一个一般的大致线性关系的构象熵的变化后,配体结合和整体结合熵的贡献。虽然这种关系不是必需的,但它的存在表明进化已经在蛋白质-配体热力学的优化中利用了构象熵。这个想法将使用最近校准的“熵计”,依赖于构象熵的动态代理定量探索。内部蛋白质运动的全面测量将进行使用现在完善的解决方案NMR弛豫方法。为了更全面地了解蛋白质基质内动力学的传播,将对蛋白质运动进行高压微扰研究,以阐明运动的耦合。最近的工作,其他人的分解代谢物激活蛋白表明,构象熵可能发挥核心作用,在蛋白质-DNA识别。因此,我们建议检查的结构和动力学特性的基础蛋白质-DNA识别的乳糖阻遏物。乳糖阻遏物是原核生物中遗传调控的范例。总的来说,这些研究将极大地扩展我们对蛋白质运动的本质以及它在蛋白质功能能量学中所代表的构象熵的作用的理解。

项目成果

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A. JOSHUA WAND其他文献

A. JOSHUA WAND的其他文献

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{{ truncateString('A. JOSHUA WAND', 18)}}的其他基金

Improving Fragment Based Drug Discovery and the Development of Tools for Chemical Biology through Nanoscale Encapsulation and NMR Spectroscopy
通过纳米级封装和核磁共振波谱改善基于片段的药物发现和化学生物学工具的开发
  • 批准号:
    10419416
  • 财政年份:
    2022
  • 资助金额:
    $ 35.04万
  • 项目类别:
Improving Fragment Based Drug Discovery and the Development of Tools for Chemical Biology through Nanoscale Encapsulation and NMR Spectroscopy
通过纳米级封装和核磁共振波谱改善基于片段的药物发现和化学生物学工具的开发
  • 批准号:
    10707914
  • 财政年份:
    2022
  • 资助金额:
    $ 35.04万
  • 项目类别:
The role of the free energy landscape in Parkin's function and dysfunction in health and disease
自由能景观在健康和疾病中帕金功能和功能障碍中的作用
  • 批准号:
    9883915
  • 财政年份:
    2020
  • 资助金额:
    $ 35.04万
  • 项目类别:
The role of the free energy landscape in Parkin's function and dysfunction in health and disease
自由能景观在健康和疾病中帕金功能和功能障碍中的作用
  • 批准号:
    10577825
  • 财政年份:
    2020
  • 资助金额:
    $ 35.04万
  • 项目类别:
The role of the free energy landscape in Parkin's function and dysfunction in health and disease
自由能景观在健康和疾病中帕金功能和功能障碍中的作用
  • 批准号:
    10356030
  • 财政年份:
    2020
  • 资助金额:
    $ 35.04万
  • 项目类别:
Nanoscale Encapsulation for Fragment Based Drug Discovery
用于基于片段的药物发现的纳米级封装
  • 批准号:
    9241998
  • 财政年份:
    2016
  • 资助金额:
    $ 35.04万
  • 项目类别:
Sensitivity enhancement in solution NMR through dynamic nuclear polarization
通过动态核极化提高溶液 NMR 的灵敏度
  • 批准号:
    8875018
  • 财政年份:
    2013
  • 资助金额:
    $ 35.04万
  • 项目类别:
Sensitivity enhancement in solution NMR through dynamic nuclear polarization
通过动态核极化提高溶液 NMR 的灵敏度
  • 批准号:
    8575416
  • 财政年份:
    2013
  • 资助金额:
    $ 35.04万
  • 项目类别:
Sensitivity enhancement in solution NMR through dynamic nuclear polarization
通过动态核极化提高溶液 NMR 的灵敏度
  • 批准号:
    8729503
  • 财政年份:
    2013
  • 资助金额:
    $ 35.04万
  • 项目类别:
Fluctuations and entropy in the energetics and function of protein complexes
蛋白质复合物的能量学和功能中的波动和熵
  • 批准号:
    8345729
  • 财政年份:
    2012
  • 资助金额:
    $ 35.04万
  • 项目类别:

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