Animal Models

使用基因工程小鼠和大鼠（例如转基因、敲除、敲入、条件性 转基因等）因为动物模型已经广泛应用于生物学的几乎所有方面。在IDDRC内的科学家进行的许多项目中，基因工程小鼠或大鼠被用作动物模型，以确定基因的功能，细胞/组织功能的基本生物学机制，和/或用作疾病或行为异常的动物模型。这些动物要么是在加州大学洛杉矶分校生产的，要么是从其他机构进口的，用于建立当地的繁殖基地。随着转基因动物的供应和使用的大量增加，随之而来的是需要核心服务，以帮助个别科学家创造、繁殖、进口、基因分型和评估小鼠和大鼠品系。许多（如果不是大多数）获得这些小鼠的科学家以前没有处理过这些动物的繁殖和基因分型。尽管如此，他们需要能够利用这些动物模型，将他们的研究工作扩展到重要的新领域。出于这些原因，我们建立了核心服务，以教育调查人员并执行这些活动。为研究者提供的服务的性质将因其复杂程度而异，每位研究者的核心参与程度将取决于各个实验室的需求。

The use of genetically-engineered mice and rats (e.g. transgenic, knock-out, knock-in, conditional transgenic, etc.) as animal models has become widespread in almost all aspects of biology. In many of the projects carried out by scientists within the IDDRC, genetically-engineered mice or rats are being used as animal models to determine the function of genes, fundamental biological mechanisms of cell/tissue function, and/or to use as animal models of disease or behavioral abnormalities. These animals have either been generated here at UCLA or they have been imported from other institutions and used to establish local breeding colonies. With this large increase in the availability and use of transgenic animals, there is a concomitant need for core services to help individual scientists create, breed, import, genotype and evaluate mouse and rat lines. Many, if not most, of the scientists obtaining these mice have not had to deal with breeding and genotyping of such animals before. Nonetheless, they need to be able to take advantage of these animal models to extend their research effort into important new areas. For these reasons, we have set up Core services to educate investigators about and perform these activities. The nature of the services for investigators will vary in their degree of sophistication and Core involvement for each investigator will depend upon the needs of individual laboratories.