HOX10 GENES REGULATE LUMBAR SPINAL CORD PATTERNING

HOX10 基因调节腰脊髓模式

• 批准号: 6163557
• 负责人: ELLEN M. CARPENTER
• 金额: $ 7.65万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-10 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6163557
• 关键词: developmental genetics; developmental neurobiology; gene mutation; genetically modified animals; homeobox genes; in situ hybridization; laboratory mouse; neurogenesis; spinal cord

DESCRIPTION (Adapted from applicant's description): The experiments described in this proposal are designed to examine the role of a set of three paralogous Hox genes in patterning the developing lumbar spinal cord. These genes, Hoxa10, Hoxc10, and Hoxd10 are expressed in broad domains encompassing the lumbar spinal cord and were chosen for analysis because they are a complete paralogous group and together are likely to provide all of the patterning information dictated by this spatial location within the Hox complex. Three specific aims will be addressed: 1) to define the regional and local patterns of expression of Hoxa10, Hoxc10, and Hoxd10 in the developing lumbar spinal cord, 2) to identify the populations of cells in the lumbar spinal cord which express Hoxca10, Hoxc10, or Hoxd10, and 3) to demonstrate that inactivation of Hoxa10, Hoxc10, or Hoxd10 alters the production and placement of specific cell populations in the lumbar spinal cord. These experiments will test the hypothesis that the expression and activity of genes which regulate transcription, such as Hox genes, establish the regional and cellular identity of the developing central nervous system.

描述（改编自申请人的描述）： 在这个建议中，旨在研究一组三个旁系同源的作用， Hox基因在形成发育中的腰椎脊髓中的作用。这些基因， Hoxa10、Hoxc10和Hoxd10在广泛的结构域中表达， 选择腰椎脊髓和进行分析，因为它们是一个完整的 旁系同源的群体和在一起很可能提供所有的模式 Hox复合体内的空间位置所决定的信息。三 具体目标是：1）确定区域和地方模式 Hoxa 10、Hoxc 10和Hoxd 10在发育中的腰椎中的表达 脊髓，2）以确定腰椎脊髓中的细胞群， 表达Hoxca 10、Hoxc 10或Hoxd 10，和3）证明Hoxca 10、Hoxc 10或Hoxd 10失活 Hoxa10、Hoxc10或Hoxd10改变特定细胞的产生和放置 腰椎脊髓中的细胞群。这些实验将测试 这一假说认为，调控基因的表达和活性 转录，如Hox基因，建立区域和细胞的身份 中枢神经系统的发育。