Whole genome screen for novel regulators of tissue homeostasis and regeneration

描述（由申请人提供）：组织稳态和再生新调控因子的全基因组筛选Schmidtea mediterranea是解决人类健康问题的模型系统。涡虫以其从身体碎片中再生完整动物的能力而闻名。最近的研究表明地中海S. mediterranea适用于现代细胞、分子和RNAi技术，它正在成为解决在黑胃果蝇或秀丽隐杆线虫中不容易研究的研究问题的首选模式生物，包括成体组织的伤口愈合、再生、体细胞干细胞和组织稳态。2007年，我们对地中海海鲈基因组进行了注释，并构建了一个包含基因模型的公开基因组数据库SmedGD。这项资助的目的是在高通量的基于图像的屏幕上使用地中海基因注释，用于组织再生和体内平衡的新型调节因子。为此，我们将(1)采用一系列分子和免疫学技术，包括全基因组RNAi筛选；(2)利用现有的图像处理、管理和注释工具来构建一个网络基础设施，既可以支持我们的实验，又可以将我们的结果分发给科学界。

DESCRIPTION (provided by applicant): Whole genome screen for novel regulators of tissue homeostasis and regeneration Schmidtea mediterranea is a model system for addressing human health issues. Planarians are well known for their ability to regenerate complete animals from fragments of their bodies. Following recent demonstrations that S. mediterranea is amenable to modern cell, molecular, and RNAi techniques, it is becoming the model organism of choice for addressing research questions that cannot be easily studied in Drosophila melanogaster or Caenorhabditis elegans, including wound healing of adult tissues, regeneration, somatic stem cells, and tissue homeostasis. In 2007 we annotated the S. mediterranea genome and constructed a publicly available genome database, SmedGD containing the gene models. The objective of this grant is to use the S. mediterranea gene annotations in a high-throughput image-based screen for novel regulators of tissue regeneration and homeostasis. Toward this end we will (1) employ a battery of molecular and immunological techniques, including a genome-wide RNAi screen, and (2) leverage existing image processing, management and annotation tools to construct a cyberinfrastructure that can both support our experiments and distribute our results to the scientific community.