权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular evolution of odorant receptor specificities in Drosophila

果蝇气味受体特异性的分子进化

基本信息

批准号：
8460162
负责人：
Stephanie Marie Rollmann
金额：
$ 26.68万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2016-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8460162
关键词：
Address Adult Alzheimer&apos s Disease Architecture Behavior Behavioral Behavioral Genetics Brain Chemicals Code Complex Diagnostic Discrimination Drosophila genus Drosophila melanogaster Elderly Environment Family Gene Family Genes Genetic Genetic Models Genetic Polymorphism Genetic Variation Genotype Goals Health Human Inbreeding Individual Insecta Knowledge Left Link Maintenance Measures Mediating Methods Modeling Molecular Molecular Evolution Molecular Genetics Nature Neurons Odorant Receptors Odors Pattern Perception Phenotype Physiological Population Process Quality of life Receptor Gene Research Series Shapes Single Nucleotide Polymorphism Site-Directed Mutagenesis Smell Perception Specificity System Testing Variant Vertebrates base combinatorial in vivo insight meetings mutant nervous system disorder nutrition programs receptor relating to nervous system research study response trait

项目摘要

Behavior is shaped by interactions among a large number of genes. To understand the genetic underpinnings of variation in behavior, its underlying genetic architecture must be dissected and the specific genetic polymorphisms that cause functional differences in behavior must be identified. Chemosensory behavior is an excellent system for addressing these goals because of recent advances uncovering both the genetic factors and neural coding involved. Chemosensory perception is an important human health issue. Loss of olfactory acuity in the elderly can compromise nutrition and quality of life and olfactory deficits are diagnostic of several neurological disorders, such as Alzheimer's. Human chemosensory perception is variable, with some specific anosmias genetically determined. Yet despite advances in our understanding of odor coding, the molecular basis of variation in olfactory perception at the level of the primary odorant receptors remains poorly understood. The similarity of odor coding in both vertebrates and insects suggests analogous principles underlie the organization of their olfactory systems. In both systems, families of odorant receptors are used to discriminate among a vast repertoire of odorants. The Drosophila olfactory system, however, is quantitatively less complex and the use of Drosophila readily allows for the control of genetic background and environment. This system provides us with an excellent opportunity for elucidating how molecular polymorphisms in odorant receptors contribute to variation in olfactory behavior. This application proposes three specific aims: (1) to measure natural variation in olfactory behavior in a population of wild-derived inbred lines of Drosophila melanogaster; (2) to quantify the contribution of molecular variation in odorant receptors to natural variation in behavior, and (3) to test the functional effects of allelic variation in odorant receptors. Behavioral variation to an array of odorants will be assessed and single nucleotide polymorphisms will be identified by sequencing the odorant receptor gene family. Polymorphisms contributing to natural variation in olfactory behavior will then be identified and further validated using crosses among wild-derived lines. In addition, functional studies of the underlying mechanisms that contribute to variation in olfactory behavior will be conducted. These experiments will provide new insights into how ensembles of receptors that recognize defined odorants contribute to natural variation in behavior at the level of single nucleotide polymorphisms. More broadly, the results will provide a model for understanding how genetic variation in candidate loci results in functional differences in behavior and the mechanisms underlying the origin and maintenance of variation in complex traits in nature.

行为是由大量基因之间的相互作用形成的。为了了解基因