HPLC-ICP-MS System

描述(由申请人提供)：我们申请资金，以便在马萨诸塞州总医院(MGH)的阿蒂努拉·马蒂诺斯生物医学成像中心安装一台新型的电感耦合等离子质谱仪(ICP-MS)，该仪器与高效液相色谱(HPLC)系统相连接。电感耦合等离子体质谱是一种强大的分析工具，可检测和定量多种样品中的痕量元素，被广泛接受为元素分析的首选方法。现代仪器可以同时分析多达60种元素，动态范围从万亿分之一到千分之九。高效液相界面使金属或类金属分析物能够在复杂的基质中进行形态分析，如血浆或组织匀浆。Martinos中心的双重使命是技术开发和翻译研究，涉及先进的成像技术，如磁共振成像(MRI)、正电子发射断层扫描、光学成像和脑磁图。准确的元素分析是这项工作的主要基础。例如，在分子成像探针的开发中，定量元素分析被用来表征探针，量化它们的分布、药代动力学和组织中的代谢。对于金属离子稳态成像，电感耦合等离子体质谱提供了一个重要的金标准。高效液相色谱-电感耦合等离子体质谱联用技术在新陈代谢研究中对基于金属的治疗方法的发展大有裨益。造影剂被广泛应用于体内定量成像技术的开发中，如灌注、通透性、血容量测量；这些造影剂的体外元素分析可以用于校准和验证这些方法。所要求的仪器将作为马蒂诺斯中心、更大的MGH研究社区以及大波士顿地区的其他机构的共享资源进行管理。首席调查员和咨询委员会将确保这一制度的公平和具有成本效益的运作。收购这一高效液相-电感耦合等离子体质谱将极大地惠及我们的研究界，我们预计它将在推动许多与癌症、糖尿病、药物成瘾、阿尔茨海默氏症、心脏病、肝脏疾病、中风和神经可塑性等医学相关问题的新发现方面发挥重要作用。 公共卫生评估：要求为元素分析仪提供资金，以便对生物组织中的金属和类金属进行量化和分布。由该仪器实现的分析将支持NIH资助的癌症、心脏病、糖尿病、成瘾研究、阿尔茨海默病和神经可塑性方面的研究项目。

DESCRIPTION (provided by applicant): We request funds for a new inductively coupled plasma mass spectrometer (ICP-MS) interfaced to a high- performance liquid chromatography (HPLC) system to be located at the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital (MGH). ICP-MS is a powerful analytical tool for the detection and quantification of trace elements in a broad range of sample types and is widely accepted as the method of choice for elemental analysis. Modern instrumentation can analyze up to 60 elements simultaneously with a dynamic range spanning 9 orders of magnitude from parts per trillion to parts per thousand. An HPLC interface enables speciation of metal or metalloid analytes in complex matrices like blood plasma or tissue homogenate. The dual mission of the Martinos Center is technology development and translational research involving advanced imaging techniques such as magnetic resonance imaging (MRI), positron emission tomography, optical imaging, and magnetoencephalography. Accurate elemental analysis underpins much of this work. For instance in molecular imaging probe development, quantitative elemental analysis is used to characterize the probes, quantify their distribution, pharmacokinetics, and metabolism in tissue. For imaging of metal ion homeostasis, ICP-MS provides a crucial gold standard. Development of metal-based therapeutics benefits tremendously from HPLC-ICP-MS for metabolism studies. Contrast agents are widely used in the development of quantitative in vivo imaging techniques, e.g. perfusion, permeability, blood volume measurements; ex vivo elemental analysis of these contrast agents can serve to calibrate and validate these methodologies. The requested instrumentation will be administered as a shared resource for the Martinos center, the larger MGH research community, as well as other institutions in the greater Boston area. The Principle Investigator and Advisory Committee will ensure both equitable and cost-effective operation of this system. The acquisition of this HPLC-ICP-MS will greatly benefit our research community and we expect it will play an essential role in driving many new discoveries pertaining to a host of medically related issues including cancer, diabetes, drug addiction, Alzheimer's disease, heart disease, liver disease, stroke, and neural plasticity. PUBLIC HEALTH REELEVANCE: Funds are requested for an elemental analyzer that will allow quantification and distribution of metals and metalloids in biological tissue. The analyses enabled by this instrument will underpin NIH-funded research projects in cancer, heart disease, diabetes, addiction research, Alzheimer's disease, and neuroplasticity.