HPLC-ICP-MS System

HPLC-ICP-MS系统

• 批准号: 8447659
• 负责人: Peter D Caravan
• 金额: $ 37.12万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447659
• 关键词: Advisory Committees; Alzheimer' s Disease; Area; Automobile Driving; Biological; Blood Volume; Boston; Communities; Complex; Contrast Media; Coupled; Detection; Development; Diabetes Mellitus; Drug Addiction; Drug Kinetics; Elements; Ensure; Funding; General Hospitals; Gold; Heart Diseases; High Pressure Liquid Chromatography; Homeostasis; Image; Imaging Techniques; Institution; Ions; Liver diseases; Magnetic Resonance Imaging; Magnetoencephalography; Malignant Neoplasms; Massachusetts; Measurement; Metabolism; Metals; Methodology; Methods; Mission; Neuronal Plasticity; Perfusion; Permeability; Plasma; Play; Positron-Emission Tomography; Public Health; Research; Research Personnel; Research Project Grants; Resource Sharing; Role; Sampling; Stroke; System; Therapeutic; Tissues; Trace Elements; Translational Research; United States National Institutes of Health; Work; addiction; analytical tool; base; bioimaging; cost effective; imaging probe; in vivo; instrument; instrumentation; mass spectrometer; metabolic abnormality assessment; molecular imaging; operation; optical imaging; research and development; technology development

DESCRIPTION (provided by applicant): We request funds for a new inductively coupled plasma mass spectrometer (ICP-MS) interfaced to a high- performance liquid chromatography (HPLC) system to be located at the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital (MGH). ICP-MS is a powerful analytical tool for the detection and quantification of trace elements in a broad range of sample types and is widely accepted as the method of choice for elemental analysis. Modern instrumentation can analyze up to 60 elements simultaneously with a dynamic range spanning 9 orders of magnitude from parts per trillion to parts per thousand. An HPLC interface enables speciation of metal or metalloid analytes in complex matrices like blood plasma or tissue homogenate. The dual mission of the Martinos Center is technology development and translational research involving advanced imaging techniques such as magnetic resonance imaging (MRI), positron emission tomography, optical imaging, and magnetoencephalography. Accurate elemental analysis underpins much of this work. For instance in molecular imaging probe development, quantitative elemental analysis is used to characterize the probes, quantify their distribution, pharmacokinetics, and metabolism in tissue. For imaging of metal ion homeostasis, ICP-MS provides a crucial gold standard. Development of metal-based therapeutics benefits tremendously from HPLC-ICP-MS for metabolism studies. Contrast agents are widely used in the development of quantitative in vivo imaging techniques, e.g. perfusion, permeability, blood volume measurements; ex vivo elemental analysis of these contrast agents can serve to calibrate and validate these methodologies. The requested instrumentation will be administered as a shared resource for the Martinos center, the larger MGH research community, as well as other institutions in the greater Boston area. The Principle Investigator and Advisory Committee will ensure both equitable and cost-effective operation of this system. The acquisition of this HPLC-ICP-MS will greatly benefit our research community and we expect it will play an essential role in driving many new discoveries pertaining to a host of medically related issues including cancer, diabetes, drug addiction, Alzheimer's disease, heart disease, liver disease, stroke, and neural plasticity. PUBLIC HEALTH REELEVANCE: Funds are requested for an elemental analyzer that will allow quantification and distribution of metals and metalloids in biological tissue. The analyses enabled by this instrument will underpin NIH-funded research projects in cancer, heart disease, diabetes, addiction research, Alzheimer's disease, and neuroplasticity.

描述（由申请人提供）：我们申请资金用于马萨诸塞州总医院（MGH）的Athinoula a . Martinos生物医学成像中心的新型电感耦合等离子体质谱仪（ICP-MS）与高效液相色谱（HPLC）系统的接口。ICP-MS是一种强大的分析工具，用于检测和定量痕量元素在广泛的样品类型，被广泛接受为元素分析的选择方法。现代仪器可以同时分析多达60种元素，动态范围从万亿分之一到千分之一跨越9个数量级。高效液相色谱接口使金属或类金属分析物能够在复杂的基质中形成物种，如血浆或组织匀浆。马蒂诺斯中心的双重使命是技术开发和转化研究，涉及先进的成像技术，如磁共振成像（MRI）、正电子发射断层扫描、光学成像和脑磁图。准确的元素分析是这项工作的基础。例如，在分子成像探针的开发中，定量元素分析用于表征探针，量化它们在组织中的分布、药代动力学和代谢。对于金属离子稳态成像，ICP-MS提供了一个关键的金标准。利用高效液相色谱- icp - ms进行代谢研究极大地促进了金属基疗法的发展。造影剂广泛应用于体内定量成像技术的发展，如灌注、通透性、血容量测量；这些造影剂的体外元素分析可以用于校准和验证这些方法。所要求的仪器将作为Martinos中心，更大的MGH研究社区以及大波士顿地区的其他机构的共享资源进行管理。首席调查员和咨询委员会将确保这一制度的公平和成本效益的运作。该高效液相色谱- icp - ms的收购将极大地有利于我们的研究社区，我们预计它将在推动许多与医学相关问题有关的新发现方面发挥重要作用，这些问题包括癌症、糖尿病、药物成瘾、阿尔茨海默病、心脏病、肝病、中风和神经可塑性。