HPLC-ICP-MS System

描述（由申请人提供）：我们申请资金用于一台新的电感耦合等离子体质谱仪（ICP-MS），该质谱仪与位于Athinoula A的高效液相色谱（HPLC）系统连接。马萨诸塞州总医院（MGH）马蒂诺斯生物医学成像中心。ICP-MS是一种强大的分析工具，可用于检测和定量多种样品类型中的痕量元素，并被广泛接受为元素分析的首选方法。现代仪器可以同时分析多达60种元素，动态范围跨越9个数量级，从万亿分之一到千分之一。HPLC接口能够在复杂基质（如血浆或组织匀浆）中对金属或类金属分析物进行形态分析。Martinos中心的双重使命是技术开发和转化研究，涉及先进的成像技术，如磁共振成像（MRI），正电子发射断层扫描，光学成像和脑磁图。精确的元素分析是这项工作的基础。例如，在分子成像探针开发中，定量元素分析用于表征探针，量化它们在组织中的分布、药代动力学和代谢。对于金属离子稳态的成像，ICP-MS提供了一个至关重要的金标准。HPLC-ICP-MS代谢研究极大地促进了金属治疗剂的发展。造影剂广泛用于定量体内成像技术的开发，例如灌注、渗透性、血容量测量;这些造影剂的离体元素分析可用于校准和验证这些方法。所要求的仪器将作为Martinos中心，更大的MGH研究社区以及大波士顿地区其他机构的共享资源进行管理。主要调查员和咨询委员会将确保这一制度的公平和具有成本效益的运作。该HPLC-ICP-MS的收购将大大有利于我们的研究界，我们预计它将在推动许多与医学相关问题有关的新发现方面发挥重要作用，包括癌症，糖尿病，药物成瘾，阿尔茨海默病，心脏病，肝病，中风和神经可塑性。 公共卫生救济：请求提供资金，用于购置一台元素分析仪，以便对生物组织中的金属和准金属进行量化和分布。该仪器支持的分析将支持NIH资助的癌症，心脏病，糖尿病，成瘾研究，阿尔茨海默病和神经可塑性研究项目。

DESCRIPTION (provided by applicant): We request funds for a new inductively coupled plasma mass spectrometer (ICP-MS) interfaced to a high- performance liquid chromatography (HPLC) system to be located at the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital (MGH). ICP-MS is a powerful analytical tool for the detection and quantification of trace elements in a broad range of sample types and is widely accepted as the method of choice for elemental analysis. Modern instrumentation can analyze up to 60 elements simultaneously with a dynamic range spanning 9 orders of magnitude from parts per trillion to parts per thousand. An HPLC interface enables speciation of metal or metalloid analytes in complex matrices like blood plasma or tissue homogenate. The dual mission of the Martinos Center is technology development and translational research involving advanced imaging techniques such as magnetic resonance imaging (MRI), positron emission tomography, optical imaging, and magnetoencephalography. Accurate elemental analysis underpins much of this work. For instance in molecular imaging probe development, quantitative elemental analysis is used to characterize the probes, quantify their distribution, pharmacokinetics, and metabolism in tissue. For imaging of metal ion homeostasis, ICP-MS provides a crucial gold standard. Development of metal-based therapeutics benefits tremendously from HPLC-ICP-MS for metabolism studies. Contrast agents are widely used in the development of quantitative in vivo imaging techniques, e.g. perfusion, permeability, blood volume measurements; ex vivo elemental analysis of these contrast agents can serve to calibrate and validate these methodologies. The requested instrumentation will be administered as a shared resource for the Martinos center, the larger MGH research community, as well as other institutions in the greater Boston area. The Principle Investigator and Advisory Committee will ensure both equitable and cost-effective operation of this system. The acquisition of this HPLC-ICP-MS will greatly benefit our research community and we expect it will play an essential role in driving many new discoveries pertaining to a host of medically related issues including cancer, diabetes, drug addiction, Alzheimer's disease, heart disease, liver disease, stroke, and neural plasticity. PUBLIC HEALTH REELEVANCE: Funds are requested for an elemental analyzer that will allow quantification and distribution of metals and metalloids in biological tissue. The analyses enabled by this instrument will underpin NIH-funded research projects in cancer, heart disease, diabetes, addiction research, Alzheimer's disease, and neuroplasticity.