Inductively Coupled Plasma Mass Spectrometer

摘要 我们申请资金用于一种新的电感耦合等离子体质谱仪（ICP-MS）， 高效液相色谱（UPLC）系统和激光消融（LA）系统将设在研究所 在马萨诸塞州总医院（MGH）的成像创新。ICP-MS是一种强大的分析工具， 在广泛的样品类型中检测和定量痕量元素，并被广泛接受为 元素分析的首选方法。现代仪器可以同时分析多达60种元素 其动态范围跨越从万亿分之几到千分之几的9个数量级。的UPLC 界面使得能够在复杂基质如血浆或组织中形成金属或类金属分析物 以快速分析时间匀浆。此外，激光烧蚀界面允许多元素分析 直接离开表面，例如显微镜载玻片，从而实现低微米空间分辨率。存在现有 HPLC-ICP-MS由先前的共享仪器赠款资助，被许多研究人员广泛使用， MGH和外面。然而，该仪器已使用8年，日益需要修理， 用户所需的UPLC或LA功能。 成像创新研究所的双重使命是技术开发和转化 研究涉及先进的成像技术，如磁共振成像（MRI），正电子发射 断层扫描、光学成像和超声。精确的元素分析是这项工作的基础。为 例如，在分子成像探针开发中，定量元素分析用于表征 探针，量化它们在组织中的分布、药代动力学和代谢。用于金属离子成像 ICP-MS提供了一个至关重要的金标准。开发金属基治疗剂的益处 代谢研究中的UPLC-ICP-MS和作用机制研究中的LA-ICP-MS的差异很大。 造影剂广泛用于定量体内成像技术的开发，例如灌注， 渗透性、血容量测量;这些造影剂的离体元素分析可用于 校准和验证这些方法。 所要求的仪器将作为创新研究所的共享资源进行管理， 成像，更大的MGH研究社区，以及在大波士顿地区的其他机构。的 首席调查员和咨询委员会将确保公平和具有成本效益的运作， 系统这种激光烧蚀的UPLC-ICP-MS的获得将极大地有利于我们的研究社区 我们预计它将在推动许多与医学有关的新发现方面发挥重要作用。 相关问题，包括癌症、肺部疾病、骨骼疾病、免疫学、神经系统疾病、心脏病 疾病、肝脏疾病、中风和神经可塑性。

Abstract We request funds for a new inductively coupled plasma mass spectrometer (ICP-MS) interfaced to a ultra- performance liquid chromatography (UPLC) system and a laser ablation (LA) system to be located at the Institute for Innovation in Imaging at Massachusetts General Hospital (MGH). ICP-MS is a powerful analytical tool for the detection and quantification of trace elements in a broad range of sample types and is widely accepted as the method of choice for elemental analysis. Modern instrumentation can analyze up to 60 elements simultaneously with a dynamic range spanning 9 orders of magnitude from parts per trillion to parts per thousand. A UPLC interface enables speciation of metal or metalloid analytes in complex matrices like blood plasma or tissue homogenate with fast analysis times. Additionally, a laser ablation interface allows multi-elemental analysis directly off a surface, such as a microscope slide, enabling low micron spatial resolution. There is an existing HPLC-ICP-MS funded by a prior Shared Instrument Grant that is extensively used by numerous investigators at MGH and outside. However, that instrument is 8 years old, increasingly in need of repair and does not provide the UPLC or LA capabilities that users desire. The dual mission of the Institute for Innovation in Imaging is technology development and translational research involving advanced imaging techniques such as magnetic resonance imaging (MRI), positron emission tomography, optical imaging, and ultrasound. Accurate elemental analysis underpins much of this work. For instance, in molecular imaging probe development, quantitative elemental analysis is used to characterize the probes, quantify their distribution, pharmacokinetics, and metabolism in tissue. For imaging of metal ion homeostasis, ICP-MS provides a crucial gold standard. Development of metal-based therapeutics benefits tremendously from UPLC-ICP-MS for metabolism studies and from LA-ICP-MS for mechanism of action studies. Contrast agents are widely used in the development of quantitative in vivo imaging techniques, e.g. perfusion, permeability, blood volume measurements; ex vivo elemental analysis of these contrast agents can serve to calibrate and validate these methodologies. The requested instrumentation will be administered as a shared resource for the Institute for Innovation in Imaging, the larger MGH research community, as well as other institutions in the greater Boston area. The Principle Investigator and Advisory Committee will ensure both equitable and cost-effective operation of this system. The acquisition of this UPLC-ICP-MS with laser ablation will greatly benefit our research community and we expect it will play an essential role in driving many new discoveries pertaining to a host of medically related issues including cancers, lung diseases, skeletal disorders, immunology, neurological diseases, heart diseases, liver diseases, stroke, and neural plasticity.