Tecan EVO GenePaint robot

Tecan EVO GenePaint 机器人

• 批准号: 8444976
• 负责人: Cecilia Ljungberg
• 金额: $ 21.5万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-02 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8444976
• 关键词: Cells; Communities; Country; Freedom; Gene Expression; Generations; Imagery; In Situ Hybridization; In Vitro; Interruption; Liquid substance; Manuals; Medical center; Medicine; Methods; Process; RNA; Radioactive; Research Project Grants; Resolution; Robot; Services; Slide; System; Techniques; Texas; Time; Tissue Sample; Tissues; Transcript; Validation; college; research study

DESCRIPTION (provided by applicant): The Baylor College of Medicine RNA In Situ Hybridization core is a unique and long-standing core. We are one of a few academic cores in the country that specialize in semi-automated high-throughput non-radioactive RNA in situ hybridization (ISH). One of the key steps in the study of gene expression is the validation of results obtained by in vitro gene expression methods such as microarrays. This validation typically involves the use of appropriate cell or tissue samples and ISH to confirm the in vitro results. The unique advantage of ISH over destructive expression techniques such as microarrays and quantitative PCR is the visualization of transcripts in intact tissues, giving information on precise anatomical and cellular localization. Processing a large number of slides for ISH is normally a laborious and time consuming process. We are using a Tecan Genesis GenePaint liquid handling robot that allows us to eliminate approximately 90% of the manual steps of a conventional ISH. It also allows us to add a number of necessary amplification steps to achieve results as sensitive as radioactive ISH, but with much better cellular resolution, and to achieve highly consistent and reproducible results. The Tecan Genesis GenePaint robot is crucial for the core, without which we as a core could not provide the same level of service. The current core robot is over a decade old and needs to be replaced. It is showing wear and tear after continuous use and its platform is no longer supported or serviced by Tecan. Many research projects within Baylor College of Medicine, the greater community at the Texas Medical Center, and beyond, depend on the core to help them with their in situ hybridization experiments. As the core is reliant on the function of this machine, it is imperative that our Tecan Genesis system be replaced without interruption of service, with Tecan's current generation liquid handler termed Freedom EVO GenePaint.

描述（由申请人提供）：贝勒医学院RNA原位杂交核心是一个独特而悠久的核心。我们是国内少数几个专门从事半自动高通量非放射性RNA原位杂交（ISH）的学术核心之一。基因表达研究的关键步骤之一是验证体外基因表达方法（如微阵列）获得的结果。这种验证通常包括使用适当的细胞或组织样本和ISH来确认体外结果。ISH相对于破坏性表达技术（如微阵列和定量PCR）的独特优势是完整组织中转录本的可视化，提供精确的解剖和细胞定位信息。处理大量的ISH幻灯片通常是一个费力和耗时的过程。我们使用的是Tecan Genesis GenePaint液体处理机器人，它使我们能够消除传统ISH中大约90%的手动步骤。它还允许我们添加一些必要的扩增步骤，以获得与放射性ISH一样敏感的结果，但具有更好的细胞分辨率，并获得高度一致和可重复的结果。Tecan Genesis GenePaint机器人对核心至关重要，没有它，我们作为核心无法提供同样水平的服务。目前的核心机器人已有十多年的历史，需要更换。它在连续使用后出现磨损，其平台不再由Tecan提供支持或服务。贝勒医学院（Baylor College of Medicine）、德克萨斯医学中心（Texas Medical Center）的大社区以及其他地方的许多研究项目都依赖于core来帮助他们进行原位杂交实验。由于核心依赖于该机器的功能，因此必须在不中断服务的情况下更换我们的Tecan Genesis系统，使用Tecan当前一代液体处理器Freedom EVO GenePaint。