Voltage-sensitive dye imaging setup

电压敏感染料成像装置

• 批准号: 8447278
• 负责人: DIETER JAEGER
• 金额: $ 21.57万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447278
• 关键词: Address; Amplifiers; Area; Auditory; Auditory area; Autistic Disorder; Basal Ganglia; Biology; Brain; Complex; Computer software; Crabs; Development; Dyes; Equipment; Faculty; Feedback; Funding; Ganglia; Generations; Genetic Techniques; Health; Homeostasis; Image; Imaging Techniques; Invertebrate Ganglia; Invertebrates; Leeches; Lobster; Methods; Microscope; Mus; Neurosciences; Optics; Parkinson Disease; Pattern; Research; Research Personnel; Rodent; Signal Transduction; Surveys; System; Technology; Ultrasonography; United States National Institutes of Health; Universities; Work; expectation; in vivo; innovation; interest; neuroregulation; novel; optogenetics; programs; research study; social; tool; vibration; voltage

DESCRIPTION (provided by applicant): We request funding to establish a voltage-sensitive dye (VSD) imaging setup in the Department of Biology at Emory University. This setup will greatly enhance the research capability of the neuroscience faculty in the Department with active NIH funding (Drs. Jaeger, Calabrese, Liu and Prinz). The P.I. (Dr. Jaeger) has a long track record in the research of basal ganglia and cerebellar networks. To extend this analysis to the level of cortical activity modulation due to basal ganglia and cerebellar feedback loops would be innovative, impactful, and timely. VSD imaging of mouse cortical activity in vivo will be a crucial tool to determine the temporal and spatial effects of basal ganglia and cerebellar activation on cortical activity. Similarly, the research of Dr. Liu is concerned with cortical actiity patterns, albeit the activation following social ultrasound signaling in mice on auditory circuits. VSD imaging would allow this work to progress towards a better understanding of spatial and temporal signal flow beyond primary auditory cortex. For both Dr Jaeger and Dr. Liu this technology would be particularly timely to implement soon, as the development of optogenetic stimulation techniques and genetic voltage-sensitive indicators will allow us to address important questions with an expected high impact in novel findings. The work of Drs. Calabrese and Prinz explores the function of invertebrate pattern generation circuits in the leech heartbeat circuit and crab/lobster stomatogastric ganglion, respectively. However, similarly to the question of spatial dynamics of cortical dynamics, these ganglia consist of a spatial network in the respective ganglia with complex activity patterns that can be fruitfully surveyed by voltage-sensitive dye imaging techniques. The equipment we request will allow us to flexibly conduct experiments on the spatial scale required in rodents in vivo (10x10 mm area of interest) and invertebrate ganglia (1x1 mm area of interest) by mounting a state of the art dual CMOS camera system either on an in vivo imaging setup or on an Olympus BX50 microscope. Both setups will share manipulators and amplifiers for simultaneous electrophysiological recordings. A substantial amount of the basic equipment (Olympus BX50 microscope, vibration isolation table, recording amplifiers) needed for this setup will be contributed by the major investigators, and a dedicated room is made available by the Dept. of Biology. The core item requested is the dual camera CMOS system, along with the necessary optical equipment (beam splitter, software, etc), and auxiliary hardware. The expectation from obtaining this setup is that innovative and timely methods will be available to the established research programs of the major investigators, and allow the development of junior faculty as well. The health impact is also highly relevant, in that the research by the Jaeger lab is directly applied to establishing Parkinson's disease mechanisms, work in the Liu lab is relevant to the study of autism, and work in the Calabrese and Prinz labs demonstrate fundamental mechanisms of neuromodulation and homeostasis that underlie understanding of how pathological brain activity may arise as a maladaptation of normal dynamics.

描述（由申请人提供）：我们要求资金在埃默里大学生物学系建立对电压敏感的染料（VSD）成像设置。这种设置将通过积极的NIH资助（Jaeger博士，Calabrese，Liu和Prinz博士）在部门中神经科学教师的研究能力大大提高。 P.I. （Jaeger博士）在基底神经节和小脑网络的研究中有很长的记录。将此分析扩展到由于基底神经节和小脑反馈回路引起的皮质活性调节水平，将具有创新，有影响力和及时。体内小鼠皮质活性的VSD成像将是确定基底神经节和小脑激活对皮质活性的时间和空间作用的关键工具。同样，Liu博士的研究与皮质活性模式有关，尽管在听觉电路上，小鼠社交超声信号传导的激活。 VSD成像将使这项工作能够更好地理解超出主要听觉皮层的空间和时间信号流。对于Jaeger博士和Liu博士而言，这项技术将特别及时实施，因为光遗传学刺激技术和遗传电压敏感的指标的发展将使我们能够在新发现的发现中与预期的高影响。博士的工作。 Calabrese和Prinz分别探讨了水ech心跳回路和蟹/龙虾气胃神经节中无脊椎动物图案产生电路的功能。但是，与皮质动力学的空间动力学问题类似，这些神经节由各自神经节中的空间网络组成，具有复杂的活性模式，可以通过电压敏感的染料成像技术进行良好的调查。我们要求的设备将使我们能够在体内（10x10 mm感兴趣的面积）和无脊椎动物神经节（1x1 mm感兴趣的面积）中灵活地进行实验，并通过安装艺术双CMOS摄像机系统在体内成像设置或Olympus BX50 MIRCOPER上安装。两个设置都将共享同时电生理记录的操纵器和放大器。该设置所需的大量基本设备（Olympus BX50显微镜，振动隔离台，记录放大器）将由主要研究人员贡献，并由生物学部提供专用房间。所请求的核心项目是双摄像头CMOS系统，以及必要的光学设备（光束分离器，软件等）和辅助硬件。获得此设置的期望是，主要研究人员的既定研究计划都将获得创新和及时的方法，并允许开发初级教师。健康影响也很重要，因为Jaeger Lab的研究直接用于建立帕金森氏病机制，在Liu Lab中的工作与自闭症研究有关，并且在Calabrese和Prinz Labs中起作用，证明了对神经调节和稳态的基本机制，这些机制是对正常脑部活动的神经教育和稳态的理解。