Voltage-sensitive dye imaging setup

电压敏感染料成像装置

• 批准号: 8447278
• 负责人: DIETER JAEGER
• 金额: $ 21.57万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447278
• 关键词: Address; Amplifiers; Area; Auditory; Auditory area; Autistic Disorder; Basal Ganglia; Biology; Brain; Complex; Computer software; Crabs; Development; Dyes; Equipment; Faculty; Feedback; Funding; Ganglia; Generations; Genetic Techniques; Health; Homeostasis; Image; Imaging Techniques; Invertebrate Ganglia; Invertebrates; Leeches; Lobster; Methods; Microscope; Mus; Neurosciences; Optics; Parkinson Disease; Pattern; Research; Research Personnel; Rodent; Signal Transduction; Surveys; System; Technology; Ultrasonography; United States National Institutes of Health; Universities; Work; expectation; in vivo; innovation; interest; neuroregulation; novel; optogenetics; programs; research study; social; tool; vibration; voltage

DESCRIPTION (provided by applicant): We request funding to establish a voltage-sensitive dye (VSD) imaging setup in the Department of Biology at Emory University. This setup will greatly enhance the research capability of the neuroscience faculty in the Department with active NIH funding (Drs. Jaeger, Calabrese, Liu and Prinz). The P.I. (Dr. Jaeger) has a long track record in the research of basal ganglia and cerebellar networks. To extend this analysis to the level of cortical activity modulation due to basal ganglia and cerebellar feedback loops would be innovative, impactful, and timely. VSD imaging of mouse cortical activity in vivo will be a crucial tool to determine the temporal and spatial effects of basal ganglia and cerebellar activation on cortical activity. Similarly, the research of Dr. Liu is concerned with cortical actiity patterns, albeit the activation following social ultrasound signaling in mice on auditory circuits. VSD imaging would allow this work to progress towards a better understanding of spatial and temporal signal flow beyond primary auditory cortex. For both Dr Jaeger and Dr. Liu this technology would be particularly timely to implement soon, as the development of optogenetic stimulation techniques and genetic voltage-sensitive indicators will allow us to address important questions with an expected high impact in novel findings. The work of Drs. Calabrese and Prinz explores the function of invertebrate pattern generation circuits in the leech heartbeat circuit and crab/lobster stomatogastric ganglion, respectively. However, similarly to the question of spatial dynamics of cortical dynamics, these ganglia consist of a spatial network in the respective ganglia with complex activity patterns that can be fruitfully surveyed by voltage-sensitive dye imaging techniques. The equipment we request will allow us to flexibly conduct experiments on the spatial scale required in rodents in vivo (10x10 mm area of interest) and invertebrate ganglia (1x1 mm area of interest) by mounting a state of the art dual CMOS camera system either on an in vivo imaging setup or on an Olympus BX50 microscope. Both setups will share manipulators and amplifiers for simultaneous electrophysiological recordings. A substantial amount of the basic equipment (Olympus BX50 microscope, vibration isolation table, recording amplifiers) needed for this setup will be contributed by the major investigators, and a dedicated room is made available by the Dept. of Biology. The core item requested is the dual camera CMOS system, along with the necessary optical equipment (beam splitter, software, etc), and auxiliary hardware. The expectation from obtaining this setup is that innovative and timely methods will be available to the established research programs of the major investigators, and allow the development of junior faculty as well. The health impact is also highly relevant, in that the research by the Jaeger lab is directly applied to establishing Parkinson's disease mechanisms, work in the Liu lab is relevant to the study of autism, and work in the Calabrese and Prinz labs demonstrate fundamental mechanisms of neuromodulation and homeostasis that underlie understanding of how pathological brain activity may arise as a maladaptation of normal dynamics.

描述(由申请者提供)：我们申请资助在埃默里大学生物系建立一个电压敏感染料(VSD)成像装置。这一设置将极大地增强NIH积极资助的神经科学系(Jaeger博士、Calabrese博士、Liu博士和普林茨博士)的研究能力。P.I.(Jaeger博士)在基底节和小脑网络的研究方面有着长期的记录。将这一分析扩展到由于基底节和小脑反馈环而引起的皮质活动调节的水平将是创新的、有效的和及时的。在体小鼠皮质活动的VSD成像将是确定基底节和小脑激活对皮质活动的时间和空间效应的重要工具。同样，刘博士的研究关注的是大脑皮层的活动模式，尽管在小鼠的听觉回路上，社会超声信号发出后会产生激活。 VSD成像将使这项工作朝着更好地了解初级听觉皮质以外的空间和时间信号流的方向发展。对于Jaeger博士和Liu博士来说，这项技术将特别及时地很快实施，因为光遗传刺激技术和遗传电压敏感指示器的发展将使我们能够解决重要问题，预计将对新发现产生重大影响。卡拉布雷塞博士和普林茨博士的工作分别探索了无脊椎动物模式产生电路在水蚤心跳回路和螃蟹/龙虾口胃神经节中的作用。然而，与皮质动力学的空间动力学问题类似，这些神经节由各自神经节中的空间网络组成，具有复杂的活动模式，可以通过电压敏感染料成像技术进行富有成效的观察。我们要求的设备将允许我们灵活地在活体啮齿动物(10x10毫米感兴趣区域)和无脊椎动物神经节(1x1毫米感兴趣区域)所需的空间尺度上进行实验，方法是在活体成像设备或奥林巴斯BX50显微镜上安装最先进的双CMOS摄像系统。这两个装置将共享操纵器和放大器，用于同时进行电生理记录。这一设置所需的大量基本设备(奥林巴斯BX50显微镜、隔振台、记录放大器)将由主要调查人员提供，国防部将提供一间专用房间。生物系的。申请的核心项目是双摄像头cmos系统，以及必要的光学设备(分束器、软件等)和辅助硬件。获得这一设置的期望是，创新和及时的方法将可用于主要研究人员的既定研究计划，并允许初级教师的发展。对健康的影响也是高度相关的，因为耶格实验室的研究直接应用于建立帕金森氏病的机制，刘实验室的工作与自闭症的研究相关，卡拉布雷斯和普林茨实验室的工作展示了神经调节和动态平衡的基本机制，这些机制奠定了对病理大脑活动如何作为正常动力学的不适应而产生的理解的基础。