Leica Laser Microdissection Microscope for a Shared Resource

共享资源的徕卡激光显微切割显微镜

• 批准号: 8448032
• 负责人: MARTHA CAMPBELL-THOMPSON
• 金额: $ 25.2万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-18 至 2014-06-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448032
• 关键词: AIDS related cancer; Acute; Address; Beta Cell; Biology; Cachexia; Cell Culture Techniques; Cells; Chairperson; Chronic; Computer software; Dissection; Fee-for-Service Plans; Fistula; Florida; Fluorescence; Funding; Genomics; Grant; Immune; Immune response; Immunology; Inflammation; Inflammatory; Institution; Insulin-Dependent Diabetes Mellitus; Laboratories; Lasers; Lupus; Lymphocyte; Maintenance; Medicine; Microdissection; Microscope; Mitochondria; Modeling; Natural regeneration; Operative Surgical Procedures; Pathology; Population; Process; Request for Proposals; Research; Research Personnel; Research Technics; Resource Sharing; Sampling; Science; Sepsis; Sepsis Syndrome; Techniques; Tissues; Training; Transplantation Immunology; Tube; United States National Institutes of Health; Universities; Venous; Work; college; experience; flexibility; glucose metabolism; instrument; instrumentation; islet; laser capture microdissection; molecular pathology

DESCRIPTION (provided by applicant): Laser capture microdissection has become a primary research technique to isolate specific cell populations from tissues or cell culture affording further sub-cellular techniques to be conducted on highly purified cells rather than a heterogeneous tissue. This proposal requests support for the purchase of a Leica LMD7000 laser capture microscope, to be maintained in an institutional Shared Resource, the Molecular Pathology Core. Access to this instrumentation is crucial to the work of two major user groups, namely, Center for Immunology and Transplantation and Laboratory of Inflammation Biology and Surgical Sciences, at the University of Florida. Administration, maintenance, and high-level technical assistance and training will be provided by experienced staff in the Molecular Pathology Core. All user training and biosafety issues have been addressed. Strong institutional commitment is demonstrated by the support of College of Medicine Dean, Dr. Michael Good, and Senior Associate Dean, Dr. Steven Sugrue, and Department of Pathology, Immunology and Laboratory Medicine Chairman, Dr. Michael Clare-Salzler. The Leica LMD7000 was selected as the most suitable for a multi-user facility due to its greater flexibility for a wide range of sampe types and clearly fulfills an unmet need as the institution does not have this instrumentation available through a shared resource. The Leica instrument has several ideal features including intuitive and simple software to automate sample dissection, 4-tube extraction capabilities, brightfield and fluorescence, cell culture, and superior objectives for sharp laser cutting. The major users highlighted in this proposal are well-funded by the NIH with active and pending grants requiring this instrumentation. Each would benefit greatly from having institutional access to a laser capture instrument. Martha Campbell-Thompson and Mark Atkinson will use the LMD7000 to study infiltrating lymphocytes and islets to understand mechanisms of beta cell destruction and islet regeneration for type 1 diabetes. Clayton Mathews will conduct research on the basic biology of mitochondria in beta cells and altered glucose metabolism through genomic analyses of isolated islets. Laurence Morel studies various immune cell populations in a model of systemic lupus and is currently subcontracting the laser capture studies to a collaborator at another institution. Lyle Moldawer studies host responses to acute inflammatory processes in sepsis and systemic inflammatory response syndromes and to chronic inflammatory processes, including cancer and AIDS-associated cachexia. He too has active NIH funding for studies with laser capture microdissection for arterial venous fistula. Finally, the instrument will be availabl to other investigators through this shared resource using a fee-for-service mechanism for training and assisted or unassisted use.

描述（由申请人提供）：激光捕获微分解已成为一种主要的研究技术，可以将特定细胞群体与组织或细胞培养的分离，从而提供进一步的亚细胞技术，可在高度纯化的细胞上进行，而不是在高度纯化的细胞上进行。该建议要求支持购买Leica LMD7000激光捕获显微镜，该显微镜将维持在机构共享资源（分子病理学核心）中。 使用该仪器对于两个主要用户群体的工作至关重要，即佛罗里达大学炎症生物学和外科科学的免疫学和移植和实验室中心。 分子病理学核心的经验丰富的员工将提供管理，维护和高级技术援助和培训。所有用户培训和生物安全问题均已解决。医学院院长，迈克尔·古德（Michael Good）博士和高级副院长，史蒂文·苏格鲁（Steven Sugrue）博士以及病理学，免疫学和实验室医学主席迈克尔·克莱尔·萨尔茨勒（Michael Clare-Salzler）的病理学系，史蒂文·苏格鲁（Steven Sugrue）博士，史蒂文·苏格鲁（Steven Sugrue）博士，史蒂文·苏格鲁（Steven Sugrue）博士以及病理学系迈克尔·克莱尔·萨尔茨勒（Michael Clare-Salzler）的支持证明了强大的机构承诺。 Leica LMD7000被选为最适合多用户设施的设施，这是因为其对各种Sampe类型的灵活性更大，并且显然满足了未满足的需求，因为该机构没有通过共享资源可用的该仪器。 Leica仪器具有几个理想的功能，包括直观和简单的软件，可自动化样品解剖，4管提取功能，Brightfield和荧光，细胞培养以及尖锐激光切割的卓越目标。该提案中强调的主要用户由NIH充分资金，并获得了需要这种仪器的积极和待处理的赠款。每个人都可以从机构访问激光捕获工具中受益匪浅。玛莎·坎贝尔·汤普森（Martha Campbell-Thompson）和马克·阿特金森（Mark Atkinson）将使用LMD7000来研究浸润淋巴细胞和胰岛，以了解1型糖尿病的β细胞破坏机制和胰岛再生的机制。克莱顿·马修斯（Clayton Mathews）将对β细胞中线粒体的基本生物学进行研究，并通过分离的胰岛的基因组分析改变葡萄糖代谢。劳伦斯·莫雷尔（Laurence Morel）在系统性狼疮模型中研究了各种免疫细胞种群，目前正在将激光捕获研究分包给另一机构的合作者。 Lyle Moldawer的研究对败血症和全身性炎症反应综合征中的急性炎症过程以及对慢性炎症过程（包括癌症和艾滋病相关的恶病质）的反应产生了反应。他也有积极的NIH资金用于研究动脉静脉瘘的激光捕获显微解剖。最后，该工具将通过此共享资源使用培训费用的机制以及辅助或无助使用的费用机制来提供给其他调查人员。