In Situ Sampling Tool for Assessing Bioavailability and Toxicity of Sediments

用于评估沉积物生物利用度和毒性的原位采样工具

• 批准号: 8514608
• 负责人: Nancy D Denslow
• 金额: $ 27.13万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8514608
• 关键词: Address; Adsorption; Amendment; Analytical Chemistry; Arizona; Behavior; Bioavailable; Biological; Biological Assay; Biological Availability; Body Burden; Carbon; Chemical Structure; Chemicals; Computer-Aided Design; Consumption; DNA Microarray Chip; Data; Data Analyses; Detection; Devices; Dichlorodiphenyl Dichloroethylene; Dieldrin; Effectiveness; Environment; Environmental Pollutants; Equilibrium; Family; Farming environment; Filtration; Fishes; Florida; Funding; Gene Expression; Goals; Hazardous Waste Sites; Health; Home environment; Hour; Human; In Situ; Laboratories; Laboratory Study; Legal patent; Mass Spectrum Analysis; Measurement; Measures; Methods; Microarray Analysis; Minnows; Modeling; Monitor; Organism; Parents; Pathway interactions; Performance; Phase; Procedures; Process; Protocols documentation; Pump; Research Project Grants; Risk; Risk Assessment; Sampling; Series; Signal Recognition Particle; Site; Solid; Stainless Steel; Superfund; Surface; System; Techniques; Technology; Testing; Time; Toxic effect; Triclosan; Tubular formation; Universities; Water; Water Pollutants; Work; adverse outcome; aged; base; bioaccumulation; design; exposed human population; field study; fipronil; high throughput screening; mathematical model; novel; novel strategies; physical property; pollutant; programs; prototype; receptor; remediation; research study; response; simulation; superfund site; tandem mass spectrometry; theories; tool; toxicant; triclocarban; uptake; water flow; water sampling

ABSTRACT This project introduces a novel strategy for bioavailability determination of sediment-borne contaminants featuring a wide range of chemical structures, properties and physical-chemical behaviors. The project addresses the pressing need of Superfund stakeholders to determine in a convenient and reliable fashion both human health risks from contaminated sediments and the effectiveness of implemented remediation strategies. The in situ sampling/bioavailability determination (IS2B) tool is a novel, patent-pending device enabling simultaneous determination of contaminant levels in bulk water and pore water at hitherto unattainably low method detection limits (MDLs). When deployed, one half of the tubular IS2B device is buried in sediment and the other is exposed to bulk water. Integrated multi-channel pumps simultaneously draw bulk water and sediment pore water into the active sampling device and push it at, respectively, high and low desirable flow rates through an array of filters and adsorption media. Water samples either can be stored in the device or expelled into the bulk water at will. Due to unlimited access to pore and bulk water during deployment, the IS2B tool provides ultra-low MDLs for a broad spectrum of contaminants, ranging from fully water-soluble to highly sorptive and hydrophobic. This functionality distinguishes the device from presently available passive sampling strategies. Know-how from three previous SRP projects is being leveraged to test the working hypothesis that the IS2B technology can serve to reliably (i) sample bulk and pore water pollutants for analysis in the sub-ng/L range, (ii) inform on the bioavailability and bioactivity of sediment contaminants, (iii) inform on human exposures and associated health risks from fish consumption, and (iv) track the progress of sediment remediation activities. Laboratory and field studies will be conducted with contaminated sediments from Lake Apopka, home to one of Florida's various Superfund sites. To illustrate the breadth of IS2B applicability, this project concentrates on two traditional and three emerging sediment contaminants (p,p'-DDE, dieldrin versus fipronil, triclosan, triclocarban). Because there may be discrepancies between the bioaccumulation predicted via modeling from sampler data and actual bioaccumulation, bioavailability and bioaccumulation of pollutants will be assessed in lab experiments with freshly spiked and long-term aged contaminated sediments via determination of body burdens in two test organisms: Lumbriculus variegatus and Pimephales promelas. Biological responses to contaminant exposure will also be measured in fish using DNA microarray analysis to evaluate effects that may not be predicted solely based on body burden of parent compounds. Mathematical relationships between pollutant concentrations in bulk water, pore water, worms and fish will be formulated from theory and lab data, and applied to IS2B-derived data to predict risk and track the effectiveness of two remediation approaches, granular activated carbon (GAC) amendment and deep tilling of contaminated sediment.

抽象的 该项目引入了一种测定沉积物污染物生物有效性的新策略 具有广泛的化学结构、性质和物理化学行为。项目 满足超级基金利益相关者的迫切需求，以方便可靠的方式确定 受污染沉积物造成的人类健康风险以及所实施的补救策略的有效性。 原位采样/生物利用度测定 (IS2B) 工具是一种新颖的、正在申请专利的设备，能够 同时测定迄今为止难以达到的低水平的散装水和孔隙水中的污染物水平 方法检测限 (MDL)。部署时，管状 IS2B 装置的一半埋在沉积物中， 另一个暴露于大量水。集成多通道泵同时抽取大量水和 将孔隙水沉积到主动采样装置中，并分别以高和低所需流量推入 通过一系列过滤器和吸附介质的速率。水样可以存储在设备中或 随意排入大量水中。由于在部署过程中不受限制地进入孔隙和散装水， IS2B 工具为多种污染物提供超低 MDL，范围从完全水溶性到 高吸附性和疏水性。此功能将该设备与目前可用的无源设备区分开来。 抽样策略。 之前三个 SRP 项目的专业知识正在被用来测试 IS2B 的工作假设 技术可以可靠地（i）对大体积和孔隙水污染物进行采样，以进行亚纳克/升范围内的分析，（ii） 通报沉积物污染物的生物利用度和生物活性，(iii) 通报人类接触情况和 鱼类消费带来的相关健康风险，以及 (iv) 跟踪沉积物修复活动的进展。 将对阿波普卡湖的受污染沉积物进行实验室和实地研究，阿波普卡湖是一处湖泊的所在地。 佛罗里达州的各个超级基金网站。为了说明 IS2B 适用性的广度，该项目重点关注 两种传统的和三种新兴的沉积物污染物（p,p'-DDE、狄氏剂与氟虫腈、三氯生、 三氯卡班）。因为通过模型预测的生物累积性之间可能存在差异 采样器数据和污染物的实际生物累积、生物利用度和生物累积将在 通过身体测定，对新鲜加标和长期陈化的污染沉积物进行实验室实验 两种测试生物体的负担：杂色Lumbriculus variegatus 和Pimephales promelas。生物反应 还将使用 DNA 微阵列分析来测量鱼类的污染物暴露情况，以评估可能产生的影响 不能仅根据母体化合物的身体负荷来预测。之间的数学关系 散装水、孔隙水、蠕虫和鱼类中的污染物浓度将根据理论和实验室数据制定， 并应用于 IS2B 派生数据来预测风险并跟踪两种补救方法的有效性， 颗粒活性炭（GAC）改良和污染沉积物的深耕。