Combination of Nanofiber Scaffolds with Gradations in Fiber Organization and Mine

描述(由申请人提供)：这项题为“将纤维组织和矿物质含量分级的纳米纤维支架与脂肪来源干细胞用于肩袖损伤修复的组合”的赠款提案解决了设计修复肩袖损伤的组织结构方面的挑战。我们将在目前的拨款提案中解决三个方面的研究问题：1)开发新型纳米纤维支架，概括肌腱到骨骼植入部位的固有结构和组成；2)利用纳米纤维支架调节脂肪干细胞(ADSCs)的行为，包括形态、增殖、迁移和分化；以及3)使用大鼠肩部损伤模型测试ADSCs种植的纳米纤维支架的有效性。为了实现我们的目标，我们正在利用一个独特的机会，将马歇尔大学两个研究小组的专业知识结合起来，以确保在拟议的资助期内实现高水平的生产力。谢博士的实验室在制造纳米纤维支架和检测细胞与纳米纤维支架之间的相互作用方面拥有丰富的经验，包括成人干细胞和胚胎干细胞的培养和分化。舒勒博士在肩袖损伤的动物模型和临床治疗方面拥有丰富的经验。两个研究小组都拥有立即开展拟议工作所需的必要技能和技术。

DESCRIPTION (provided by applicant): This grant proposal entitled "Combination of Nanofiber Scaffolds with Gradations in Fiber Organization and Mineral Content and Adipose-derived Stem Cells for Rotator Cuff Injury Repair" addresses the challenge in engineering a tissue construct to repair rotator cuff injury. The research issues we will address in the current grant proposal are three-fold: 1) Developing novel nanofiber scaffolds that will recapitulate the native structure and composition of tendon-to-bone insertion site, 2) Regulating the behaviors of adipose- derived stem cells (ADSCs) including morphology, proliferation, migration, and differentiation using nanofiber scaffolds, and 3) Testing the efficacy of ADSCs seeded nanofiber scaffolds using a rat shoulder injury model. To address our objectives, we are taking advantage of a unique opportunity to combine expertise from two research groups at Marshall University to ensure high level of productivity during the proposed funding period. Dr. Xie's laboratory has extensive experience in fabrication of nanofiber scaffolds and examination of interaction between cells and nanofiber scaffolds including culture and differentiation of adult and embryonic stem cells. Dr. Shuler has extensive experience in the animal model and clinical treatment of rotator cuff injury. Both research groups have the necessary skills and technology required to immediately carry out the work proposed.