Electron Microscopy

电子显微镜

• 批准号: 8743793
• 负责人: Guofeng Zhang
• 金额: $ 14.17万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8743793
• 关键词: Actins; Autophagocytosis; Biological; Biological Process; Cell Line; Cells; Chemicals; Cryopreservation; Cytoskeleton; DNA Sequence Rearrangement; Dehydration; Development; Drosophila genus; Drug Delivery Systems; Electron Microscopy; Freeze Substitution; Freezing; Institutes; Link; Membrane Fusion; Methods; Mitochondria; Molecular; Morphology; Mucins; National Heart, Lung, and Blood Institute; National Institute of Biomedical Imaging and Bioengineering; National Institute of Dental and Craniofacial Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Neurological Disorders and Stroke; Negative Staining; Neurons; Organelles; Oxidative Stress; Procedures; Process; Publishing; Research Personnel; Sampling; Staining method; Stains; Structure; System; Techniques; Transmission Electron Microscopy; United States National Institutes of Health; Universities; cell motility; digital; gastrointestinal system; glycosylation; medical schools; nanoparticle; pressure; sample fixation

We have used conventional chemical fixation, dehydration, embedding and staining procedures to carry out studies on several fundamental biological processes, including membrane fusion, macroautophagy, and motility. We established a number of collaborative projects with multiple institutes across NIH. For example, with NIA we performed studies on the neuroprotective mechanism of NAM, the NAD+ precursor, and its effects on the autophagy-system in neurons exposed to oxidative stress; with NIDCR we examined mucin-type O-linked glycosylation in the structures of digestive system during development in Drosophila; and with NHLBI we investigated the process of selective degradation of mitochondria by using Drosophila cell lines. We also collaborated with a group in The Johns Hopkins University School of Medicine to use a high-pressure freezing/freeze-substitution method, the most useful and powerful cryofixation method, to study dynamic rearrangements of the actin cytoskeleton in Drosophila. The findings were published in the May issue of Developmental Cell. In addition, quite a few projects involving the characterization of nanoparticles and drug delivery mechanisms were performed by using electron microscopy techniques, including negative staining methods. Investigators within the IRPs of NIBIB, NIDDK and NINDS initiated most of these projects.

我们使用传统的化学固定、脱水、包埋和染色程序对几个基本的生物过程进行研究，包括膜融合、巨自噬和运动。我们与 NIH 的多个机构建立了许多合作项目。例如，我们通过 NIA 研究了 NAM（NAD+ 前体）的神经保护机制及其对暴露于氧化应激的神经元自噬系统的影响；我们与 NIDCR 一起检查了果蝇发育过程中消化系统结构中的粘蛋白型 O 连接糖基化；通过 NHLBI，我们利用果蝇细胞系研究了线粒体选择性降解的过程。 我们还与约翰霍普金斯大学医学院的一个小组合作，使用高压冷冻/冷冻替代方法（最有用和最强大的冷冻固定方法）来研究果蝇肌动蛋白细胞骨架的动态重排。研究结果发表在《发育细胞》五月号上。 此外，相当多涉及纳米颗粒表征和药物输送机制的项目是通过使用电子显微镜技术（包括负染色方法）进行的。 NIBIB、NIDDK 和 NINDS 的 IRP 内的研究人员发起了大部分此类项目。