Electron Microscopy

电子显微镜

• 批准号: 9794611
• 负责人: Guofeng Zhang
• 金额: $ 33.37万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9794611
• 关键词: Actins; Autophagocytosis; Biological; Biological Process; Cell Line; Cells; Cryopreservation; Cytoskeleton; Dehydration; Development; Drosophila genus; Drug Delivery Systems; Electron Microscopy; Exposure to; Freeze Substitution; Freezing; Institutes; Link; Membrane Fusion; Methods; Mitochondria; Molecular; Morphology; Mucins; National Heart, Lung, and Blood Institute; National Institute of Biomedical Imaging and Bioengineering; National Institute of Dental and Craniofacial Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Neurological Disorders and Stroke; Negative Staining; Neurons; Organelles; Oxidative Stress; Procedures; Process; Publishing; Research Personnel; Sampling; Stains; Structure; System; Techniques; Transmission Electron Microscopy; United States National Institutes of Health; Universities; cell motility; chemical fixation; digital; gastrointestinal system; glycosylation; medical schools; nanoparticle; nanoparticle delivery; pressure; sample fixation

We have used conventional chemical fixation, dehydration, embedding and staining procedures to carry out studies on several fundamental biological processes, including membrane fusion, macroautophagy, and motility. We established a number of collaborative projects with multiple institutes across NIH. For example, with NIA we performed studies on the neuroprotective mechanism of NAM, the NAD+ precursor, and its effects on the autophagy-system in neurons exposed to oxidative stress; with NIDCR we examined mucin-type O-linked glycosylation in the structures of digestive system during development in Drosophila; and with NHLBI we investigated the process of selective degradation of mitochondria by using Drosophila cell lines. We also collaborated with a group in The Johns Hopkins University School of Medicine to use a high-pressure freezing/freeze-substitution method, the most useful and powerful cryofixation method, to study dynamic rearrangements of the actin cytoskeleton in Drosophila. The findings were published in the May issue of Developmental Cell. In addition, quite a few projects involving the characterization of nanoparticles and drug delivery mechanisms were performed by using electron microscopy techniques, including negative staining methods. Investigators within the IRPs of NIBIB, NIDDK and NINDS initiated most of these projects.

我们已经使用了传统的化学固定，脱水，包埋和染色程序进行研究的几个基本的生物过程，包括膜融合，macroautophagy，和运动。我们与NIH的多个研究所建立了许多合作项目。例如，使用NIA，我们研究了NAM（NAD+前体）的神经保护机制及其对暴露于氧化应激的神经元中自噬系统的影响;使用NIDCR，我们研究了果蝇发育期间消化系统结构中的粘蛋白型O-连接糖基化;使用NHLBI，我们研究了使用果蝇细胞系选择性降解线粒体的过程。 我们还与约翰霍普金斯大学医学院的一个小组合作，使用高压冷冻/冷冻替代方法，最有用和最强大的冷冻固定方法，研究果蝇肌动蛋白细胞骨架的动态重排。研究结果发表在5月出版的《发育细胞》上。 此外，相当多的项目涉及纳米粒子和药物输送机制的表征进行了使用电子显微镜技术，包括负染色方法。 NIBIB，NIDDK和NINDS的IRP内的调查人员发起了大多数这些项目。