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Electron Microscopy

电子显微镜

基本信息

批准号：
10919052
负责人：
Guofeng Zhang
金额：
$ 126.26万
依托单位：
NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10919052
关键词：
Actins Autophagocytosis Biological Biological Process Cell Line Cells Collaborations Cryopreservation Cytoskeleton Dehydration Development Drosophila genus Drug Delivery Systems Electron Microscopy Exposure to Freeze Substitution Freezing Link Membrane Fusion Methods Mitochondria Molecular Morphology Mucins National Heart, Lung, and Blood Institute National Institute of Biomedical Imaging and Bioengineering National Institute of Dental and Craniofacial Research National Institute of Diabetes and Digestive and Kidney Diseases National Institute of Neurological Disorders and Stroke Negative Staining Neurons Organelles Oxidative Stress Procedures Process Publishing Research Personnel Sampling Stains Structure System Techniques Transmission Electron Microscopy United States National Institutes of Health Universities cell motility chemical fixation digital gastrointestinal system glycosylation medical schools nanoparticle neuroprotection pressure sample fixation

项目摘要

We have used conventional chemical fixation, dehydration, embedding and staining procedures to carry out studies on several fundamental biological processes, including membrane fusion, macroautophagy, and motility. We established a number of collaborative projects with multiple institutes across NIH. For example, with NIA we performed studies on the neuroprotective mechanism of NAM, the NAD+ precursor, and its effects on the autophagy-system in neurons exposed to oxidative stress; with NIDCR we examined mucin-type O-linked glycosylation in the structures of digestive system during development in Drosophila; and with NHLBI we investigated the process of selective degradation of mitochondria by using Drosophila cell lines. We also collaborated with a group in The Johns Hopkins University School of Medicine to use a high-pressure freezing/freeze-substitution method, the most useful and powerful cryofixation method, to study dynamic rearrangements of the actin cytoskeleton in Drosophila. The findings were published in the May issue of Developmental Cell. In addition, quite a few projects involving the characterization of nanoparticles and drug delivery mechanisms were performed by using electron microscopy techniques, including negative staining methods. Investigators within the IRPs of NIBIB, NIDDK and NINDS initiated most of these projects.

我们使用传统的化学固定、脱水、包埋和染色程序对几个基本的生物过程进行研究，包括膜融合、巨自噬和运动。我们与 NIH 的多个机构建立了许多合作项目。例如，我们通过 NIA 研究了 NAM（NAD+ 前体）的神经保护机制及其对暴露于氧化应激的神经元自噬系统的影响；我们与 NIDCR 一起检查了果蝇发育过程中消化系统结构中的粘蛋白型 O 连接糖基化；通过 NHLBI，我们利用果蝇细胞系研究了线粒体选择性降解的过程。我们还与约翰霍普金斯大学医学院的一个小组合作，使用高压冷冻/冷冻替代方法（最有用和最强大的冷冻固定方法）来研究果蝇肌动蛋白细胞骨架的动态重排。研究结果发表在《发育细胞》五月号上。此外，相当多涉及纳米颗粒表征和药物输送机制的项目是通过使用电子显微镜技术（包括负染色方法）进行的。 NIBIB、NIDDK 和 NINDS 的 IRP 内的研究人员发起了大部分此类项目。

项目成果

期刊论文数量（16）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nuclear mapping of nanodrug delivery systems in dynamic cellular environments.

DOI：
10.1021/nn300516g
发表时间：
2012-06-26
期刊：
ACS nano
影响因子：
17.1
作者：
Bhirde AA;Kapoor A;Liu G;Iglesias-Bartolome R;Jin A;Zhang G;Xing R;Lee S;Leapman RD;Gutkind JS;Chen X
通讯作者：
Chen X

Monoubiquitination of EEA1 regulates endosome fusion and trafficking.

DOI：
10.1186/2045-3701-3-24
发表时间：
2013-05-23
期刊：
Cell & bioscience
影响因子：
7.5
作者：
Ramanathan HN;Zhang G;Ye Y
通讯作者：
Ye Y

Combining quantitative 2D and 3D image analysis in the serial block face SEM: application to secretory organelles of pancreatic islet cells.

DOI：
10.1111/jmi.12276
发表时间：
2015-08
期刊：
Journal of microscopy
影响因子：
2
作者：
Shomorony A;Pfeifer CR;Aronova MA;Zhang G;Cai T;Xu H;Notkins AL;Leapman RD
通讯作者：
Leapman RD

The genotype-dependent influence of functionalized multiwalled carbon nanotubes on fetal development.

DOI：
10.1016/j.biomaterials.2013.10.027
发表时间：
2014-01
期刊：
BIOMATERIALS
影响因子：
14
作者：
Huang, Xinglu;Zhang, Fan;Sun, Xiaolian;Choi, Ki-Young;Niu, Gang;Zhang, Guofeng;Guo, Jinxia;Lee, Seulki;Chen, Xiaoyuan
通讯作者：
Chen, Xiaoyuan

The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM.