Microbial interactions in otitis media

中耳炎中的微生物相互作用

• 批准号: 8441597
• 负责人: WILLIAM E SWORDS
• 金额: $ 28.63万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8441597
• 关键词: Accounting; Address; Affect; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Bacteria; Childhood; Chinchilla (genus); Chronic; Clinical; Communities; Data; Diagnosis; Disease; Environment; Haemophilus influenzae; Immune response; Immunity; In Vitro; Incidence; Individual; Infection; Inflammatory Response; Knowledge; Learning; Liquid substance; Microbial Biofilms; Modeling; Moraxella (Branhamella) catarrhalis; Office Visits; Operative Surgical Procedures; Organism; Otitis Media; Outcome; Pathogenesis; Patients; Population; Prevalence; Public Health; Recurrence; Resistance; Sampling; Severities; Severity of illness; Streptococcus pneumoniae; Treatment Failure; Tube; Tympanostomy; base; clinical practice; clinically relevant; economic impact; improved; in vivo; insight; microorganism interaction; middle ear; novel; novel strategies; pathogen; patient population; prevent; response

PROJECT SUMMARY This project aims to define how polymicrobial infection influences bacterial otitis media. Haemophilus influenzae (Hi), Streptococcus pneumoniae (Sp), and Moraxella catarrhalis (Mcat) are the predominant isolates from patients with OM, and a wealth of evidence indicates that OM often involves combinations of these organisms. We hypothesize that interactions between bacterial species can impact OM disease parameters that include bacterial persistence, resistance to clearance and antibiotic treatment, and the initiation, progression and severity of the host inflammatory response. In order to address this hypothesis we will complete the following Specific Aims: Specific Aim 1: To define interactions among bacterial pathogens causing OM. Specific Aim 2. To ask how different interspecies relationships affect experimental OM. Specific Aim 3. To evaluate impact of polymicrobial infection on antibiotic resistance. Specific Aim 4. To establish the incidence of coinfection in patients undergoing tympanostomy. Most of our current knowledge about bacterial pathogenesis is derived from infection studies using pure cultures that may not fully represent how infections occur in actual patients. This proposal presents an opportunity to learn how multiple pathogens inhabiting the same ecological niche influence one another in biofilm communities that are critical to persistent infections.

项目摘要 该项目旨在确定多种微生物感染如何影响细菌性中耳炎。嗜血杆菌 流感（Hi）、肺炎链球菌（Sp）和卡他莫拉菌（Mcat 大量的证据表明，OM通常涉及这些因素的组合， 有机体我们假设细菌种类之间的相互作用可以影响OM疾病 参数，包括细菌持久性、对清除和抗生素治疗的抗性，以及 宿主炎症反应的开始、进展和严重程度。为了解决这一 假设我们将完成以下具体目标： 具体目标1：确定引起OM的细菌病原体之间的相互作用。 具体目标2。不同的物种间关系如何影响实验OM。 具体目标3。评价多种微生物感染对抗生素耐药性的影响。 具体目标4。确定鼓膜造孔术患者合并感染的发生率。 我们目前关于细菌致病机理的知识大多来自于感染研究， 纯培养物可能不能完全代表感染在实际患者中是如何发生的。该提案提出 有机会了解居住在同一生态位的多种病原体如何影响一种病原体 另一个在生物膜群落中，这对持续感染至关重要。