Biomolecular Nuclear Magnetic Resonance Shared Resource

生物分子核磁共振共享资源

• 批准号: 8378937
• 负责人: MARY C. BECKERLE
• 金额: $ 4.73万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8378937
• 关键词: Advisory Committees; Arts; Biochemistry; Biological Factors; Biomolecular Nuclear Magnetic Resonance; Budgets; Cancer Center; Cancer Center Support Grant; Cell Differentiation process; Cells; Colorado; Communities; Computer software; Data; Data Collection; Developmental Therapeutics Program; Device or Instrument Development; Diagnostic Imaging; Doctor of Philosophy; Drug Delivery Systems; Faculty; Fee-for-Service Plans; Funding; Institutes; Laboratories; Leadership; Maintenance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Methods; Molecular Structure; NMR Spectroscopy; Natural Product Drug; Nuclear; Nuclear Magnetic Resonance; Nucleic Acids; Organic Chemicals; Peer Review; Play; Polymers; Process; Proteins; Regulation; Research; Research Personnel; Resolution; Resource Sharing; Resources; Role; Sampling; Schedule; Services; Staging; Structure; Supervision; Surveys; System; Techniques; Technology; The Sun; Therapeutic; Time; Training; United States National Institutes of Health; Universities; Utah; Work; analytical method; base; cell growth; computerized data processing; drug development; innovation; instrument; instrumentation; interest; macromolecule; magnetic field; member; nucleic acid structure; polypeptide; professor; programs; repaired; research study; response; satisfaction; small molecule

The Biomolecular Nuclear Magnetic Resonance (BNMR) Shared Resource provides a broad range of NMRbased services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The Resource provides high-level technical support and access to state-of-the-art NMR software and hardware, including spectrometers operating at 400, 500, 600, 800, and 900 MHz for protein/nucleic acid structure determination as well as routine analytical NMR services. The first three instruments are located at the University of Utah in centralized space and the latter two instruments in Colorado-based regional system (Boulder, 800; Denver, 900). The 400 MHz spectrometer is dedicated to small molecule, organic, and natural products NMR applications. The 500 MHz and 600 MHz spectrometers were recently upgraded to increase BNMR Shared Resource capability. The 600 MHz spectrometer is the highest field spectrometer in Utah. The BNMR Shared Resource manages Sun, SGI, and LINUX workstations for data processing, analysis, and structure determination through the Colorado-based regional system. Commonly used software for NMR spectroscopy is provided, including CYANA2.1, FELIX2004, MOLMOL, NMRPIPE, PYMOL, SPARKY, VNMRJ,andXPLOR-NIH. The BNMR Shared Resource is directed by Jack Skalicky, PhD, a Research Assistant Professor in Biochemistry, who provides technical support for the most demanding NMR projects and assists researchers with data collection, processing, and analysis. The Resource has a full-time NMR technician responsible for training new users, performing scheduled maintenance, and recording small-molecule NMR experiments on the 400 and 500 MHz spectrometers. A shared hardware technician provides repairs. The Resource serves members of all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers) to characterize molecules, particulariy proteins, that play roles in cancer mechanisms. In addition, characterization of small compounds, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The BNMR Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). Use of the BNMR Shared Resource by Cancer Center members with peer-reviewed funding is 60 percent. Funds are requested from the CCSG to cover 16 percent ($42,543) of the proposed Resource budget. The BNMR Shared Resource has ample capacity for additional use by Cancer Center members.

生物分子核磁共振共享资源提供了广泛的基于生物分子的核磁共振 为亨斯迈癌症研究所(HCI)成员和整个犹他大学研究提供服务 社区。该资源提供高级技术支持和访问最先进的核磁共振软件 硬件，包括工作在400、500、600、800和900 MHz的蛋白质/核酸光谱仪 结构测定以及常规的核磁共振分析服务。前三台仪器位于 集中空间中的犹他大学和科罗拉多州区域系统中的后两个工具 (博尔德，800；丹佛，900)。400 mhz光谱仪专用于小分子、有机和天然物质。 产品核磁共振应用。500兆赫和600兆赫光谱仪最近进行了升级，以增加 B核磁共振共享资源功能。600 MHz光谱仪是犹他州场强最高的光谱仪。这个 BNMR共享资源管理Sun、SGI和Linux工作站以进行数据处理、分析和 通过科罗拉多州的区域系统确定结构。核磁共振常用软件 提供光谱学，包括CyanA2.1、FELIX2004、MOLMOL、NMRPIPE、PYMOL、SPAKY、 VNMRJ和XPLOR-NIH。 B核磁共振共享资源由杰克·斯卡利基博士指导，他是 生物化学，为要求最苛刻的核磁共振项目提供技术支持并协助研究人员 进行数据收集、处理和分析。该资源有一名全职核磁共振技术人员负责 培训新用户，执行计划维护，并记录小分子核磁共振实验 400兆赫和500兆赫光谱仪。共享硬件技术人员提供维修服务。 该资源为所有基于HCI实验室的科学项目(细胞的核控制)的成员提供服务 生长和分化；细胞反应和调节；成像、诊断和治疗；以及 胃肠道癌症)来描述在癌症中起作用的分子，特别是蛋白质 机制。此外，小分子化合物的特性，包括天然产物和药物输送 聚合物，对HCI的发育治疗活动有重要贡献。 BNMR共享资源是一个机构管理的设施，由大学和 人机界面领导力。有一个学院咨询委员会，并通过以下方式审查资源以确保用户满意 年度调查。该设施是按服务收费(按存储容量使用计费)运营的。共享的B核磁共振的使用 癌症中心成员提供的资源与同行评审的资金比例为60%。向以下机构申请资金 CCSG将涵盖拟议资源预算的16%(42,543美元)。B核磁共振共享资源具有 有足够的容量供癌症中心成员额外使用。