"Characterization of the germ plasm of the WASP Nasonic Vitripennis"

“WASP Nasonic Vitripennis 种质的表征”

• 批准号: 8619890
• 负责人: Jeremy Lynch
• 金额: $ 7.82万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-24 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8619890
• 关键词: Algorithms; Animal Model; Animals; Area; Behavior; Binding; Bioinformatics; Biology; Blastoderm; Cell Nucleus; Cell physiology; Cells; Cellular biology; Characteristics; Cytoplasm; Cytoplasmic Granules; Detection; Development; Developmental Biology; Disease; Drosophila genus; Educational Status; Embryo; Embryonic Development; Epithelial; Epithelium; Funding; Gene Expression Profile; Generations; Genes; Genetic Transcription; Genome; Genomics; Germ; Germ Cells; Goals; Human; In Situ Hybridization; Insecta; Knowledge; Medical; Mentors; Messenger RNA; Methods; Modeling; Molecular; Morphology; Nature; Neurons; Organelles; Polar Regions; Principal Investigator; Process; Proteins; RNA; RNA Interference; RNA Sequences; Recruitment Activity; Regulation; Ribonucleoproteins; Sampling; Specificity; Stem cells; Structure; Structure of primordial sex cell; Students; Techniques; Technology; Testing; Time; Touch sensation; Transcript; Vertebrates; Wasps; Work; base; cell behavior; cell type; egg; experience; gastrulation; gene function; graduate student; improved; interest; mRNA Expression; neuronal cell body; next generation sequencing; novel; particle; pluripotency; polarized cell; programs; public health relevance; undergraduate student

DESCRIPTION (provided by applicant): The main objective of this proposal is to provide a complete characterization of the RNA composition germ plasm of the wasp Nasonia vitripennis, and to then to begin the process of functionally testing confirmed factors localized to this organelle. Understanding the molecules that make up this structure is important since germ plasm has the unique capability to drive naive nuclei rapidly into the germline developmental program. Many of the characteristics of primordial germ cells are of general interest to both development and disease, including periods of general transcriptional quiescence, the potential for pluripotency, and trans-epithelial migratory behavior. The Nasonia germ plasm is of particular interest, as it is an unusually large, discrete ribonucleoprotein entity, in contrast tothe smaller granules characteristic of other germ plasms. In addition, the primordial germ cells of Nasonia behave differently from those of Drosophila, indicating a divergent molecular make-up of this entity in the wasp. Established techniques for isolating embryo fragments from Drosophila will be applied to Nasonia embryos. Fragments highly enriched for germ plasm material will be compared directly to corresponding fragments depleted for the material, by using quantitative RNA sequencing technologies. Those transcripts that are significantly overrepresented in the germ plasm containing fraction compared to the depleted fraction will be candidate components of the germ plasm. These will be confirmed by in situ hybridization, and by functional testing with RNAi. This study will touch on the fields of developmental biology, RNA regulation, cell biology, genomics, and evolutionary biology. This work will include the intense participation of a graduate student, who will be recruited in the coming year, and may also involve an undergraduate student. It is expected that the students will be at the early training level of experience, and the Principal Investigator will invest significant time in trainig and mentoring the recruited student(s).

描述（由申请人提供）：本提案的主要目的是提供一个完整的表征的RNA组成种质的黄蜂Nasonia vitripennis，然后开始的过程中，功能测试已确认的因素定位于这个细胞器。了解构成这种结构的分子是重要的，因为种质具有独特的能力，可以将幼稚细胞核迅速驱动到种系发育程序中。原始生殖细胞的许多特征对发育和疾病都具有普遍意义，包括一般转录静止期、多能性潜力和跨上皮迁移行为。Nasonia种质特别令人感兴趣，因为它是一个异常大的离散核糖核蛋白实体，与其他种质的较小颗粒特征相反。此外，Nasonia的原始生殖细胞的行为与果蝇的原始生殖细胞不同，表明黄蜂中这种实体的分子组成不同。从果蝇中分离胚胎碎片的既定技术将应用于Nasonia胚胎。通过使用定量RNA测序技术，将高度富集种质材料的片段直接与相应的耗尽材料的片段进行比较。与耗尽级分相比，在含种质级分中显著过量表达的那些转录物将是种质的候选组分。这些将通过原位杂交和RNAi功能测试来证实。这项研究将涉及发育生物学，RNA调控，细胞生物学，基因组学和进化生物学领域。这项工作将包括一名研究生的积极参与，他们将在明年被招募，也可能涉及一名本科生。预计学生将处于早期培训经验水平，主要研究者将投入大量时间培训和指导招募的学生。