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"Characterization of the germ plasm of the WASP Nasonic Vitripennis"

“WASP Nasonic Vitripennis 种质的表征”

基本信息

批准号：
8739308
负责人：
Jeremy Lynch
金额：
$ 7.61万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-24 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8739308
关键词：
Algorithms Animal Model Animals Area Behavior Binding Bioinformatics Biology Blastoderm Cell Nucleus Cell physiology Cells Cellular biology Characteristics Cytoplasm Cytoplasmic Granules Detection Development Developmental Biology Disease Drosophila genus Educational Status Embryo Embryonic Development Epithelial Epithelium Funding Gene Expression Profile Generations Genes Genetic Transcription Genome Genomics Germ Germ Cells Goals Human In Situ Hybridization Insecta Knowledge Medical Mentors Messenger RNA Methods Modeling Molecular Morphology Nature Neurons Organelles Polar Regions Principal Investigator Process Proteins RNA RNA Interference RNA Sequences Recruitment Activity Regulation Ribonucleoproteins Sampling Specificity Stem cells Structure Structure of primordial sex cell Students Techniques Technology Testing Time Touch sensation Transcript Vertebrates Wasps Work base cell behavior cell type egg experience gastrulation gene function graduate student improved interest mRNA Expression neuronal cell body next generation sequencing novel particle pluripotency polarized cell programs public health relevance undergraduate student

项目摘要

DESCRIPTION (provided by applicant): The main objective of this proposal is to provide a complete characterization of the RNA composition germ plasm of the wasp Nasonia vitripennis, and to then to begin the process of functionally testing confirmed factors localized to this organelle. Understanding the molecules that make up this structure is important since germ plasm has the unique capability to drive naive nuclei rapidly into the germline developmental program. Many of the characteristics of primordial germ cells are of general interest to both development and disease, including periods of general transcriptional quiescence, the potential for pluripotency, and trans-epithelial migratory behavior. The Nasonia germ plasm is of particular interest, as it is an unusually large, discrete ribonucleoprotein entity, in contrast tothe smaller granules characteristic of other germ plasms. In addition, the primordial germ cells of Nasonia behave differently from those of Drosophila, indicating a divergent molecular make-up of this entity in the wasp. Established techniques for isolating embryo fragments from Drosophila will be applied to Nasonia embryos. Fragments highly enriched for germ plasm material will be compared directly to corresponding fragments depleted for the material, by using quantitative RNA sequencing technologies. Those transcripts that are significantly overrepresented in the germ plasm containing fraction compared to the depleted fraction will be candidate components of the germ plasm. These will be confirmed by in situ hybridization, and by functional testing with RNAi. This study will touch on the fields of developmental biology, RNA regulation, cell biology, genomics, and evolutionary biology. This work will include the intense participation of a graduate student, who will be recruited in the coming year, and may also involve an undergraduate student. It is expected that the students will be at the early training level of experience, and the Principal Investigator will invest significant time in trainig and mentoring the recruited student(s).

描述(由申请人提供)：本提案的主要目的是提供一份完整的黄蜂的RNA组成种质的特征，然后开始对该细胞器中已证实的因子进行功能测试。了解构成这种结构的分子很重要，因为种质具有独特的能力，可以迅速将幼核驱动到种系发育程序中。原始生殖细胞的许多特征对发育和疾病都是普遍感兴趣的，包括一般转录静止期、多能性的可能性和跨上皮迁移行为。与其他种质所特有的较小颗粒不同，纳斯尼亚种质是一种异常大的、离散的核糖核蛋白实体，因此特别令人感兴趣。此外，Nasonia的原始生殖细胞与果蝇的原始生殖细胞的行为不同，表明黄蜂中这一实体的分子构成存在分歧。已建立的从果蝇中分离胚胎片段的技术将应用于Nasonia胚胎。通过使用定量RNA测序技术，高度浓缩的种质材料片段将直接与相应的耗尽种质材料的片段进行比较。那些在含有组分的种质中显著高于枯竭组分的转录本将成为种质的候选成分。这些将通过原位杂交和RNAi的功能测试来证实。这项研究将涉及发育生物学、RNA调控、细胞生物学、基因组学和进化生物学等领域。这项工作将包括一名研究生的密集参与，该研究生将在来年招聘，还可能涉及一名本科生。预计学生将处于早期培训经验水平，首席调查员将投入大量时间培训和指导被录取的学生(S)。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Global analysis of dorsoventral patterning in the wasp Nasonia reveals extensive incorporation of novelty in a regulatory network.

对黄蜂 Nasonia 背腹模式的整体分析揭示了调控网络中广泛的新颖性。

DOI：
10.1186/s12915-016-0285-y
发表时间：
2016
期刊：
BMC biology
影响因子：
5.4
作者：
Pers,Daniel;Buchta,Thomas;Özüak,Orhan;Wolff,Selma;Pietsch,JessicaM;Memon,MohammadBilal;Roth,Siegfried;Lynch,JeremyA
通讯作者：
Lynch,JeremyA

Emerging developmental genetic model systems in holometabolous insects.