Animal Model Core

项目总结（见说明）： 路易斯维尔大学分子靶标COBRE核心D的目标是通过使用转基因癌症小鼠模型，促进癌症预防，诊断和治疗的新方法的快速转化。尤其是。核心D将通过开发协调的资源，技术，专业知识和几个转基因小鼠群体来促进路易斯维尔大学COBRE-related研究人员的翻译目标，用于新型抗癌方法的安全性和有效性测试。最初的目标将是建立转基因小鼠的群体，并开发用于纵向监测肿瘤进展或消退的方法，而无需对动物实施安乐死。一旦核心建立，长期目标将是为路易斯维尔大学的初级和高级癌症研究人员提供转基因小鼠模型，包括过去和现在的COBRE研究人员，以促进新型抗癌化合物和策略的测试。具体目标是：㈠维持、分发和提供黑色素瘤、儿童神经母细胞瘤、肺癌、乳腺癌、前列腺癌、结肠癌、胰腺癌和血液癌转基因小鼠模型的临床前毒性和疗效支持; ㈡为转基因小鼠基因分型、标本收集和处理以及新型抗癌剂的PK分析提供技术支持;及（iii）提供最先进的影像诊断服务，以检测肿瘤及对新治疗药物的反应。总之，我们提出了一个核心设施，将采购和繁殖几种常见癌症类型的转基因小鼠模型，并将提供技术服务，以促进路易斯维尔大学开发的新策略和药物的测试。

PROJECT SUMMARY (See instructions): The goal of the University of Louisville Molecular Targets COBRE Core D is to facilitate the rapid translation of novel approaches to cancer prevention, diagnosis and treatment through the use of transgenic mouse models of cancer. In particular. Core D will facilitate the translational objectives of University of Louisville COBRE-related investigators by developing coordinated resources, techniques, expertise and several colonies of transgenic mice for the safety and efficacy testing of novel anti-cancer approaches. The initial goal will be to establish colonies of the transgenic mice and to develop methods for longitudinal monitoring of tumor progression or regression without the need to euthanize the animals. Once the core is established, the long-term objectives will be to provide access of transgenic mouse models of cancer to junior and senior cancer researchers at the University of Louisville, including past and present COBRE investigators, in order to facilitate the testing of novel anti-cancer compounds and strategies. The specific objectives are to: (i) Maintain, distribute and provide pre-clinical toxicity and efficacy support for transgenic mouse models of melanoma, childhood neuroblastoma, lung, breast, prostate, colon, pancreatic and hematological cancers; (ii) Provide technical support for transgenic mouse genotyping, specimen collection and processing, and PK analyses of novel anti-cancer agents; and (iii) Conduct state-of-the-art diagnostic imaging services for the detection of tumors and response to novel therapeutic agents. In summary, we propose a core facility that will procure and breed transgenic mouse models of several common cancer types and that will provide technical services to facilitate the testing of novel strategies and drugs developed at the University of Louisville.