Methodology for the Synthesis of Structurally Homogeneous N-Linked Glycopeptides
结构均质的 N-连接糖肽的合成方法
基本信息
- 批准号:8947805
- 负责人:
- 金额:$ 23.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-01 至 2019-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAmino AcidsAnti-HIV AgentsAntineoplastic AgentsBiologicalBiological FactorsBiological ModelsBiological ProcessCarbohydratesClinicalCollaborationsCommunitiesDevelopmentEnsureEventFertilizationGlycobiologyGlycopeptidesGlycoproteinsGlycosidesHealthHydrazineImmune responseInfectionInflammationInstitutionKnowledgeLibrariesLinkMediatingMethodologyMethodsModificationMolecularMorphologic artifactsNitrogenOligosaccharidesOutcomeOximesPeptidesPharmaceutical PreparationsPhasePlayPolysaccharidesPropertyProtein BiosynthesisPublic HealthReactionResearchRoleSchemeSideSolidSpecificityStructureStudentsTissuesUniversitiesVeinsWorkappendagecareerdrug discoveryfunctional groupglycosylationimprovednovelprogramsprotein foldingprotein functionsugartoolundergraduate researchundergraduate student
项目摘要
DESCRIPTION (provided by applicant): This project involves the development of methodologies that provide convenient access to homogeneous glycoproteins. Specifically, the PI and his undergraduate research team at Seattle University (SU) will develop a novel, efficient de novo synthesis of N-linked glycopeptides that employs ß-glycosylhydrazides, generated via hydrazide glycosylation, as ß-glycosylamine surrogates. The proposed method to synthesize N-linked glycopeptides will harness hydrazide glycosylation reactions to provide glycopeptides with natural glycosidic linkages and with greater efficiency than traditional methods. Current oxyamine and hydrazide glycosylation methods produce glycoproteins with non-natural N-linked glycoproteins containing oxime, oxyamine, or hydrazine glycan-peptide linkages and thus current methods make it difficult for chemists and biologists to study with confidence the influence of glycan structure on protein function. The PI chose to pursue his independent career at SU, a predominantly undergraduate institution, so that his research program could simultaneously impact the scientific community and transform the lives of undergraduate students. All of the research the PI has performed since he began his career at SU in 2005 has been done in collaboration with undergraduate research students; undergraduate students will work side-by-side with the PI on all aspects of the work proposed here.
描述(由申请人提供):该项目涉及开发方法,提供方便的同质糖蛋白。具体而言,PI及其在西雅图大学(SU)的本科研究团队将开发一种新型、有效的N-连接糖肽从头合成方法,该方法采用通过酰肼糖基化生成的β-糖基酰肼作为β-葡糖胺替代物。 所提出的合成N-连接糖肽的方法将利用酰肼糖基化反应来提供具有天然糖苷键的糖肽,并且比传统方法具有更高的效率。目前的羟胺和酰肼糖基化方法产生具有含有肟、羟胺或肼聚糖-肽键的非天然N-连接糖蛋白的糖蛋白,因此目前的方法使得化学家和生物学家难以有信心地研究聚糖结构对蛋白质功能的影响。 PI选择在SU(一个主要的本科院校)从事他的独立职业,这样他的研究项目就可以同时影响科学界并改变本科生的生活。PI自2005年在SU开始职业生涯以来所做的所有研究都是与本科研究生合作完成的;本科生将与PI在这里提出的工作的各个方面并肩工作。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Generation of a glycosylated asparagine residue through chemoselective acylation of a glycosylhydrazide.
通过糖基酰肼的化学选择性酰化生成糖基化天冬酰胺残基。
- DOI:10.1016/j.carres.2020.108022
- 发表时间:2020
- 期刊:
- 影响因子:3.1
- 作者:Rykaczewski,KatieA;Sabourin,KateE;Goo,PaulJ;Griggs,LydiaH;Jain,Saumya;Reed,PaxtonAM;Langenhan,JosephM
- 通讯作者:Langenhan,JosephM
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOSEPH M LANGENHAN其他文献
JOSEPH M LANGENHAN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOSEPH M LANGENHAN', 18)}}的其他基金
相似海外基金
Double Incorporation of Non-Canonical Amino Acids in an Animal and its Application for Precise and Independent Optical Control of Two Target Genes
动物体内非规范氨基酸的双重掺入及其在两个靶基因精确独立光学控制中的应用
- 批准号:
BB/Y006380/1 - 财政年份:2024
- 资助金额:
$ 23.11万 - 项目类别:
Research Grant
Quantifying L-amino acids in Ryugu to constrain the source of L-amino acids in life on Earth
量化 Ryugu 中的 L-氨基酸以限制地球生命中 L-氨基酸的来源
- 批准号:
24K17112 - 财政年份:2024
- 资助金额:
$ 23.11万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Molecular recognition and enantioselective reaction of amino acids
氨基酸的分子识别和对映选择性反应
- 批准号:
23K04668 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Basic research toward therapeutic strategies for stress-induced chronic pain with non-natural amino acids
非天然氨基酸治疗应激性慢性疼痛策略的基础研究
- 批准号:
23K06918 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanisms how arrestins that modulate localization of glucose transporters are phosphorylated in response to amino acids
调节葡萄糖转运蛋白定位的抑制蛋白如何响应氨基酸而被磷酸化的分子机制
- 批准号:
23K05758 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Design and Synthesis of Fluorescent Amino Acids: Novel Tools for Biological Imaging
荧光氨基酸的设计与合成:生物成像的新工具
- 批准号:
2888395 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Studentship
Collaborative Research: RUI: Elucidating Design Rules for non-NRPS Incorporation of Amino Acids on Polyketide Scaffolds
合作研究:RUI:阐明聚酮化合物支架上非 NRPS 氨基酸掺入的设计规则
- 批准号:
2300890 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Continuing Grant
Structurally engineered N-acyl amino acids for the treatment of NASH
用于治疗 NASH 的结构工程 N-酰基氨基酸
- 批准号:
10761044 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Lifestyle, branched-chain amino acids, and cardiovascular risk factors: a randomized trial
生活方式、支链氨基酸和心血管危险因素:一项随机试验
- 批准号:
10728925 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别:
Single-molecule protein sequencing by barcoding of N-terminal amino acids
通过 N 端氨基酸条形码进行单分子蛋白质测序
- 批准号:
10757309 - 财政年份:2023
- 资助金额:
$ 23.11万 - 项目类别: