Quantitative Pharmacology Assay Core

本定量药理学分析(QPA)核心(C)支持名为“儿科抗菌药物治疗的发展和转化性药理学”的计划，该计划是根据RFA-HD-10-026：儿科发展药理学(RPDP)专门研究中心(U54)的申请提交的。加州大学圣地亚哥分校拟议项目的总体主题是将发育生理学、药理学和传染病领域的非临床和临床专家聚集在一起，并进行转化性研究，以推动儿科发育药理学领域的发展。QPA核心将为加州大学圣地亚哥分校儿科发展药理学研究(RPDP)中心项目和试点项目提供及时、准确和成本效益高的定量分析。它将采用已发表的分析方法，并开发新的方法来测量药物、其代谢物和作为抗菌肽的药物，使用适合儿科人群的小样本体积。这些检测将覆盖各种基质，包括血浆、细胞内、尿液和组织。有多种方法可用，包括带有紫外线检测的高效液相色谱、放射免疫法、LC/MS/MS和GC/MS。QPA Core根据CLIA保持医生执照，并参与NIAID的临床药理学质量保证计划。实验室将与PM Core密切合作，确定检测灵敏度要求，以便使用最具成本效益的方法。QPA核心还将与培训和推广核心合作，为学生、住院医生和研究员提供分析方法培训机会。

This Quantitative Pharmacology Assay (QPA) Core (C) supports the program entitled "Developmental and Translational Pharmacology of Pediatric Antimicrobial Therapy", submitted as an application responsive to RFA-HD-10-026: Specialized Center in Research in Pediatric Developmental Pharmacology (RPDP) Program (U54). The overall theme of the proposed program at UC San Diego is to bring together non-clinical and clinical experts in the fields of developmental physiology, pharmacology, and infectious diseases and conduct translational research to advance the field of pediatric developmental pharmacology. The QPA Core will provide timely, accurate and cost effective quantitative assays in support of the UC San Diego Research in Pediatric Development Pharmacology (RPDP) Center Projects and Pilot Projects. It will adopt published analytical methods and develop de-novo approaches to measure drugs, their metabolites and as antimicrobial peptides using small sample volumes appropriate for pediatric populations. These assay will cover various matrices including, plasma, intracellular, urine and tissue. Various methodologies are available including HPLC with UV detection, RIA, LC/MS/MS, and GC/MS. The QPA Core maintains medical licensure under CLIA and participates in NIAID's Clinical Pharmacology Quality Assurance program. The laboratory will work closely with the PM Core to determine assay sensitivity requirements so the most cost effective method can be used. The QPA Core will also collaborate with the Training & Outreach Core to provide assay methodology training opportunities for students, residents and fellows.