Developing a Non-invasive Monitoring System Using NIR Dyes Inside Erythrocytes

使用红细胞内部近红外染料开发非侵入性监测系统

• 批准号: 8529511
• 负责人: MARK A MILANICK
• 金额: $ 17.69万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8529511
• 关键词: Affect; Animal Model; Animals; Area; Bilirubin; Biological Sciences; Biomedical Engineering; Biosensor; Blood; Blood Volume; Blood specimen; Cartoons; Cell physiology; Cells; Chemistry; Chronic; Clinical; Collaborations; Coupling; Cytolysis; Detection; Development; Devices; Diabetes Mellitus; Disease; Dyes; Encapsulated; Erythrocytes; Fluorescent Dyes; Fructose; Glass; Glucose; Goals; Health; Home environment; Hormones; Human; Immune system; Implant; Indium; Knowledge; Label; Leadership; Life; Measurement; Measures; Metabolic; Methodology; Methods; Modeling; Monitor; Optical Instrument; Optics; Organ; Patients; Persons; Physiologic pulse; Physiological; Plasma; Rattus; Research; Right-On; Running; Signal Transduction; Skin; Structure; System; Techniques; Technology; Testing; Time; Tissues; Variant; biomaterial compatibility; chemical synthesis; cost; design and construction; flexibility; handbook; improved; in vivo; innovation; instrument; instrumentation; interest; neuronal cell body; non-invasive monitor; novel; novel strategies; prevent; ratiometric; research study; response; sensor; simulation; theories; tool; transmission process

DESCRIPTION (provided by applicant): This collaborative proposal combines recent advances in the areas of red blood cell encapsulation, noninvasive optical monitoring and chemical synthesis of optical probes to develop a new technique that allows long term noninvasive monitoring of key blood parameters. To enable this novel methodology, this is the first attempt to encapsulate optical sensing elements in red blood cells (RBCs) to monitor plasma parameters. Combining the biomedical optics knowledge of the Meissner group, the RBC physiology knowledge of the Milanick group and the chemical synthesis abilities of the Glass group will result in the demonstration of a revolutionary noninvasive optical monitoring technique that can be broadly applied to long term continuous monitoring. Encapsulating optical sensing dyes inside RBCs and then returning the cells to the animal or person will for the first time enable continuous noninvasive optical monitoring for months in vivo. Leveraging expertise in biomedical engineering, chemistry and life sciences, the objectives of our collaboration are first to develop novel NIR fluorescent sensors and load them into RBCs using its reversible lysis pore, second to develop optical instrumentation for sensor interrogation with an integrated theory/experiment approach, third to test the loaded RBC's and the developed instrumentation in a rat model. The project will result in the development and proof-of-concept of a revolutionary biomedical sensing technique that employs functionalized RBCs for long term monitoring of blood analytes. A noninvasive monitoring system for blood parameters would have high impact on disease/physiological tracking for chronic conditions such as diabetes. Unlike conventional techniques, the innovative proposed sensing system utilizes cells from the body, RBCs that live for 120 days, to prevent immune system responses. With optical probes inside RBCs, circulating RBCs will provide constant sampling of blood analytes and overcome the major impediment to conventional implantable monitors. Significant knowledge and techniques about loading RBCs with optically active sensors will also be developed. By optically coupling the sensors, the technique enables noninvasive interrogation of blood analytes at little or no measurement cost. This facilitates frequent testing for both on-demand and continuous monitoring.

描述（由申请人提供）：该合作提案结合了红细胞包封、无创光学监测和光学探针化学合成领域的最新进展，以开发一种新技术，允许长期无创监测关键血液参数。为了实现这种新的方法，这是首次尝试将光学传感元件封装在红细胞（RBC）中以监测血浆参数。结合迈斯纳集团的生物医学光学知识，米兰尼克集团的红细胞生理学知识和玻璃集团的化学合成能力，将导致一个革命性的非侵入性光学监测技术，可广泛应用于长期连续监测的示范。将光学传感染料封装在红细胞内，然后将细胞送回动物或人体内，将首次实现连续数月的体内非侵入性光学监测。利用生物医学工程，化学和生命科学方面的专业知识，我们合作的目标是首先开发新型NIR荧光传感器并使用其可逆裂解孔将其加载到RBC中，其次开发用于传感器询问的光学仪器，采用综合理论/实验方法，第三在大鼠模型中测试加载的RBC和开发的仪器。该项目将导致一种革命性的生物医学传感技术的开发和概念验证，该技术采用功能化的红细胞对血液分析物进行长期监测。用于血液参数的非侵入性监测系统将对慢性病症（诸如糖尿病）的疾病/生理跟踪具有高度影响。与传统技术不同，创新的传感系统利用体内的细胞，即存活120天的红细胞来防止免疫系统反应。通过红细胞内的光学探针，循环红细胞将提供血液分析物的恒定采样，并克服传统植入式监测器的主要障碍。还将开发关于将光学活性传感器装载到RBC的重要知识和技术。通过光学耦合传感器，该技术能够以很少或没有测量成本对血液分析物进行无创询问。这有助于频繁测试，以实现按需和连续监控。