A genetic resource for Anopheles immunity research

按蚊免疫研究的遗传资源

• 批准号: 8566400
• 负责人: David A O'Brochta
• 金额: $ 20.65万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8566400
• 关键词: Animal Model; Anopheles Genus; Attention; Biochemical; Biology; Cell Death; Collection; Communities; Culicidae; Development; Disease; Double-Stranded RNA; Drosophila melanogaster; Drug or Chemical; Enhancers; Fluorescence-Activated Cell Sorting; Gene Expression; Genes; Genetic; Goals; Growth and Development function; Health; Hemocytes; House mice; Human; Imagery; Immune; Immune response; Immune system; Immunity; Individual; Infection; Insect Vectors; Insecta; Intervention; Invaded; Investigation; Label; Malaria; Mediating; Mediator of activation protein; Methods; Nitrogen; Oxygen; Parasites; Phagocytosis; Physiological; Physiological Processes; Plasmodium; Play; Population; Predisposition; Production; Proteins; Reaction; Research; Research Personnel; Resistance; Resources; Role; Scientist; Technology; Tissues; Transgenic Organisms; Uncertainty; Vector-transmitted infectious disease; Work; antimicrobial; base; cytotoxic; design; genetic resource; genetic technology; genome sequencing; in vivo; interest; microbial; next generation sequencing; novel; overexpression; pathogen; public health relevance; response; tool; transcription factor; transmission process; vector

DESCRIPTION (provided by applicant): Insect vector-borne diseases continue to burden a large fraction of the world's population. Understanding the basic biology of the insects responsible for transmitting the pathogens that cause these diseases has been and will continue to be essential for the development of effective strategies of control. Technological advances often provide novel opportunities to enlarge our understanding of basic biology. This project will result in the creation of a genetic resource consisting of transgenic lines of the human malaria mosquito Anopheles stephensi expressing the transcription factor Gal4 specifically in hemocytes. This collection of lines will serve as a valuable tool for the investigation of the cellular immune system in mosquitoes, which consists of hemocytes that are responsible for encapsulation and phagocytosis as well as synthesis of antimicrobial proteins. This aspect of the mosquito's immune system is less well understood than the more-well-studied humoral immune system although there has been growing realization that it is an important mediator of microbial infections in these insects. This collection of lines expressing Gal4 specifically in hemocytes will be used subsequently to label hemocytes in vivo to facilitate their visualization under a variety of physiological conditions, to manipulate hemocyte gene expression (silencing, overexpression, mis-expression) to assess the genetic basis of hemocyte growth, development and/or function and to express cell-death or cytotoxic genes to specifically ablate hemocytes or subpopulations of hemocytes to assess the impact on various physiological processes.

描述（由申请人提供）：虫媒传播的疾病继续给世界上很大一部分人口带来负担。了解负责传播导致这些疾病的病原体的昆虫的基本生物学对于制定有效的控制策略一直是并将继续是至关重要的。技术进步往往提供新的机会，扩大我们对基础生物学的理解。该项目将导致创建一个遗传资源，包括在血细胞中特异性表达转录因子Gal 4的人按蚊Anopheles stephensi的转基因品系。这些细胞系将作为研究蚊子细胞免疫系统的有价值的工具，蚊子细胞免疫系统由负责包囊和吞噬作用以及合成抗菌蛋白的血细胞组成。蚊子免疫系统的这一方面比更好地研究的体液免疫系统了解得更少，尽管人们越来越认识到它是这些昆虫中微生物感染的重要媒介。这些在血细胞中特异性表达Gal 4的细胞系的集合随后将用于在体内标记血细胞，以促进它们在各种生理条件下的可视化，以操纵血细胞基因表达（沉默、过表达、错误表达）来评估血细胞生长的遗传基础，发育和/或功能，并表达细胞-死亡或细胞毒性基因来特异性地消融血细胞或血细胞亚群，以评估对各种生理过程的影响。