Genetic Analysis of Anopheles mosquitoes

按蚊的遗传分析

基本信息

  • 批准号:
    8830420
  • 负责人:
  • 金额:
    $ 31.83万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-05-01 至 2016-04-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Insect vector-borne diseases continue to burden a large fraction of the world's population. Understanding the basic biology of the insects responsible for transmitting the pathogens that cause these diseases has been and will continue to be essential for the development of effective strategies of control. Technological advances often provide novel opportunities to enlarge our understanding of basic biology. This project will result in the creation of enhancer- and gene-trap technologies for the human malaria mosquito Anopheles stephensi that will enable researchers to identify, isolate and analyze genes in new and powerful ways. The technology will be applied to the study of the salivary glands, an organ that plays a vital role in the transmission of pathogens such as Plasmodium. Preliminary results show that the transposable element piggyBac is highly active within the genome of Anopheles stephensi, making it a useful platform upon which to build these genetic technologies. Prototype enhancer- trap and gene-trap systems have been created and introduced into Anopheles stephensi. Preliminary screens to test the functionality of both systems were successfully completed. Both systems are active and effective at sensing enhancers and genes, respectively. A small diverse collection of enhancer-trap lines is described which provides a proof of principle. Based on prototype designs, existing Anopheles stephensi enhancer- and gene-trap technologies will be modified for increased efficiency and effectiveness. The transgenic lines necessary for conducting enhancer- and gene-trap screens, as well as, useful 'Gal4 driver lines' will become a community resources. Anopheles stephensi gene-trap technology will be used to identify and isolate genes specifically expressed in the salivary glands of larvae and adults under a variety of developmental and physiological conditions. This project will enable the importance of various salivary gland secretions in mosquito feeding and pathogen transmission to be understood, and to integrate knowledge concerning salivary gland structure and function. This new detailed understanding of this critical tissue in a major vector o human disease is expected to lead to the conception and implementation of new strategies for disrupting pathogen transmission.
描述(由申请人提供):虫媒传播的疾病继续给世界上很大一部分人口带来负担。了解负责传播引起这些疾病的病原体的昆虫的基本生物学对于制定有效的控制策略一直并将继续至关重要。技术进步常常提供新的机会来扩大我们对基础生物学的理解。该项目将为人类疟疾蚊子史氏按蚊创建增强子和基因捕获技术,使研究人员能够以新的、有效的方式识别、分离和分析基因。该技术将应用于唾液腺的研究,唾液腺是一种在疟原虫等病原体传播中发挥重要作用的器官。初步结果表明,转座元件piggyBac在史氏按蚊基因组中高度活跃,使其成为构建这些遗传技术的有用平台。原型增强子捕获和基因捕获系统已被创建并引入史氏按蚊。测试两个系统功能的初步屏幕已成功完成。这两个系统分别在感知增强子和基因方面活跃且有效。描述了一小部分不同的增强子捕获线,这提供了原理证明。基于原型设计,现有的斯氏按蚊增强剂和基因捕获技术将进行修改,以提高效率和效果。进行增强子和基因捕获筛选所需的转基因系以及有用的“Gal4 驱动系”将成为社区资源。史氏按蚊基因诱捕技术将用于识别和分离在各种发育和生理条件下幼虫和成虫唾液腺中特异性表达的基因。该项目将使人们了解各种唾液腺分泌物在蚊子进食和病原体传播中的重要性,并整合有关唾液腺结构和功能的知识。对人类疾病主要媒介中这一关键组织的新的详细了解预计将导致阻断病原体传播的新策略的构思和实施。

项目成果

期刊论文数量(0)
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科研奖励数量(0)
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专利数量(0)

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David A O'Brochta其他文献

David A O'Brochta的其他文献

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{{ truncateString('David A O'Brochta', 18)}}的其他基金

A genetic resource for Anopheles immunity research
按蚊免疫研究的遗传资源
  • 批准号:
    8566400
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
A genetic resource for Anopheles immunity research
按蚊免疫研究的遗传资源
  • 批准号:
    8663190
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
Genetic Analysis of Anopheles mosquitoes
按蚊的遗传分析
  • 批准号:
    8436700
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
Genetic Analysis of Anopheles mosquitoes
按蚊的遗传分析
  • 批准号:
    8654288
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
Genetic Analysis of Anopheles mosquitoes
按蚊的遗传分析
  • 批准号:
    9040864
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
Gene drive potential of transposable elements in Anopheles gambiae.
冈比亚按蚊转座元件的基因驱动潜力。
  • 批准号:
    7252066
  • 财政年份:
    2006
  • 资助金额:
    $ 31.83万
  • 项目类别:
Gene drive potential of transposable elements in Anopheles gambiae.
冈比亚按蚊转座元件的基因驱动潜力。
  • 批准号:
    8079225
  • 财政年份:
    2006
  • 资助金额:
    $ 31.83万
  • 项目类别:
Gene drive potential of transposable elements in Anopheles gambiae.
冈比亚按蚊转座元件的基因驱动潜力。
  • 批准号:
    7640580
  • 财政年份:
    2006
  • 资助金额:
    $ 31.83万
  • 项目类别:
Gene drive potential of transposable elements in Anopheles gambiae.
冈比亚按蚊转座元件的基因驱动潜力。
  • 批准号:
    7130828
  • 财政年份:
    2006
  • 资助金额:
    $ 31.83万
  • 项目类别:
Gene drive potential of transposable elements in Anopheles gambiae.
冈比亚按蚊转座元件的基因驱动潜力。
  • 批准号:
    7450809
  • 财政年份:
    2006
  • 资助金额:
    $ 31.83万
  • 项目类别:

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