A genetic resource for Anopheles immunity research

按蚊免疫研究的遗传资源

• 批准号: 8663190
• 负责人: David A O'Brochta
• 金额: $ 19万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8663190
• 关键词: Animal Model; Anopheles Genus; Attention; Biochemical; Biology; Cell Death; Collection; Communities; Culicidae; Development; Disease; Double-Stranded RNA; Drosophila melanogaster; Drug or Chemical; Enhancers; Fluorescence-Activated Cell Sorting; Gene Expression; Genes; Genetic; Goals; Growth and Development function; Health; Hemocytes; House mice; Human; Imagery; Immune; Immune response; Immune system; Immunity; Individual; Infection; Insect Vectors; Insecta; Intervention; Invaded; Investigation; Label; Malaria; Mediating; Mediator of activation protein; Methods; Nitrogen; Oxygen; Parasites; Phagocytosis; Physiological; Physiological Processes; Plasmodium; Play; Population; Predisposition; Production; Proteins; Reaction; Research; Research Personnel; Resistance; Resources; Role; Scientist; Technology; Tissues; Transgenic Organisms; Uncertainty; Vector-transmitted infectious disease; Work; antimicrobial; base; cytotoxic; design; genetic resource; genetic technology; genome sequencing; in vivo; interest; microbial; next generation sequencing; novel; overexpression; pathogen; public health relevance; response; tool; transcription factor; transmission process; vector

DESCRIPTION (provided by applicant): Insect vector-borne diseases continue to burden a large fraction of the world's population. Understanding the basic biology of the insects responsible for transmitting the pathogens that cause these diseases has been and will continue to be essential for the development of effective strategies of control. Technological advances often provide novel opportunities to enlarge our understanding of basic biology. This project will result in the creation of a genetic resource consisting of transgenic lines of the human malaria mosquito Anopheles stephensi expressing the transcription factor Gal4 specifically in hemocytes. This collection of lines will serve as a valuable tool for the investigation of the cellular immune system in mosquitoes, which consists of hemocytes that are responsible for encapsulation and phagocytosis as well as synthesis of antimicrobial proteins. This aspect of the mosquito's immune system is less well understood than the more-well-studied humoral immune system although there has been growing realization that it is an important mediator of microbial infections in these insects. This collection of lines expressing Gal4 specifically in hemocytes will be used subsequently to label hemocytes in vivo to facilitate their visualization under a variety of physiological conditions, to manipulate hemocyte gene expression (silencing, overexpression, mis-expression) to assess the genetic basis of hemocyte growth, development and/or function and to express cell-death or cytotoxic genes to specifically ablate hemocytes or subpopulations of hemocytes to assess the impact on various physiological processes.

描述（由申请人提供）：昆虫媒介传播的疾病继续给世界上很大一部分人口造成负担。了解负责传播引起这些疾病的病原体的昆虫的基本生物学已经并将继续是制定有效控制战略的必要条件。技术进步常常提供新的机会来扩大我们对基础生物学的理解。该项目将产生一种遗传资源，包括人类疟疾蚊子斯蒂芬按蚊的转基因系，在血细胞中特异性表达转录因子Gal4。这些细胞系将成为研究蚊子细胞免疫系统的宝贵工具，蚊子细胞免疫系统由负责包封和吞噬以及抗菌蛋白合成的血细胞组成。蚊子免疫系统的这一方面比研究得更充分的体液免疫系统了解得更少，尽管人们越来越认识到它是这些昆虫微生物感染的重要媒介。这些在血细胞中特异性表达Gal4的细胞系将随后用于在体内标记血细胞，以便在各种生理条件下观察它们，操纵血细胞基因表达（沉默、过表达、错表达），以评估血细胞生长的遗传基础。发育和/或功能，表达细胞死亡或细胞毒性基因，以特异性地消融血细胞或血细胞亚群，以评估对各种生理过程的影响。