Mucoadhesive Polymers for Sublingual Vaccine Delivery

用于舌下疫苗递送的粘膜粘附聚合物

• 批准号: 8446264
• 负责人: LOUISE BRAUD LAWSON
• 金额: $ 22.58万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8446264
• 关键词: Adjuvant; Animals; Antibodies; Antibody Formation; Antigens; Cells; Cessation of life; Child; Communicable Diseases; Data; Development; Disease; Disease Outbreaks; Dose; Drug Formulations; Effectiveness; Feces; Film; Foundations; Health Personnel; Immune; Immune response; Immunization; Immunologics; Injection of therapeutic agent; Intestines; Investigation; Life; Liquid substance; Mass Vaccinations; Modeling; Mucosal Immunity; Mus; Needles; Polymers; Role; Safety; Serum; Surface; Syringes; Testing; Training; Vaccine Antigen; Vaccines; Virus; cost; disease transmission; in vivo; influenza virus vaccine; novel; pandemic disease; programs; protective efficacy; response; uptake; vaccine delivery

DESCRIPTION (provided by applicant): Despite great strides in development of effective vaccines, over two million deaths each year are due to vaccine-preventable diseases, indicating the need for increased accessibility to existing vaccines. In the proposed studies, we will explore a novel platform for mucosal immunization - sublingual immunization using mucoadhesive polymer films. Our hypothesis is that sublingual vaccine delivery will induce significant and protective immune responses and incorporation within mucoadhesive dissolvable polymer films containing mucosal adjuvants can enhance this response. Using inactivated influenza vaccine as a model antigen, we will perform in vivo immunization and challenge studies in a murine model to assess antibody and cellular immune responses, virus neutralization, and protective efficacy. We will determine the effectiveness of delivering influenza vaccine plus coadministered adjuvant in a single-dose mucoadhesive dissolvable film. We expect that the mucoadhesive film will result in a reduction in the amount of antigen needed to induce an immune response that is protective upon challenge. We will also better understand the role of mucosal adjuvants in modifying the immune response. These results will provide a foundation for further exploration of dissolvable films for co-administering adjuvants with inactivated influenza vaccines and other potential vaccine formulations for induction of broad mucosal protection.

描述（由申请人提供）：尽管在有效疫苗的开发方面取得了巨大进步，但每年仍有200多万人死于疫苗可预防的疾病，这表明需要增加现有疫苗的可及性。在拟议的研究中，我们将探讨 粘膜免疫的新平台-使用粘膜粘附聚合物膜的舌下免疫。我们的假设是，舌下接种疫苗将诱导显着的保护性免疫反应，并且掺入含有粘膜佐剂的粘膜粘附可溶性聚合物膜可以增强这种反应。使用灭活流感疫苗作为模型抗原，我们将在小鼠模型中进行体内免疫和攻毒研究，以评估抗体和细胞免疫应答、病毒中和和保护效力。我们将确定在单剂量粘膜粘附可溶解膜中递送流感疫苗加共同施用的佐剂的有效性。我们预期粘膜粘附膜将导致诱导免疫应答所需的抗原量减少，所述免疫应答在攻击时具有保护性。我们还将更好地了解粘膜佐剂在调节免疫应答中的作用。这些结果将为进一步探索用于与灭活流感疫苗和其他潜在疫苗制剂共同施用佐剂以诱导广泛粘膜保护的可溶解膜提供基础。