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Liposome-Encapsulated Biodefense Vaccines

脂质体封装的生物防御疫苗

基本信息

批准号：
7407440
负责人：
LOUISE BRAUD LAWSON
金额：
$ 5.13万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7407440
关键词：
Acquired Immunodeficiency Syndrome Adult Aging Animals Anthrax disease Antibiotics Antigen Targeting Antigens Autoimmune Diseases Bioterrorism Birds Carrier Proteins Cause of Death Cells Ceramides Cessation of life Chemicals Child Communicable Diseases Dendritic Cells Deposition Drug Formulations Drug resistance Electricity Encapsulated Enhancers Epidermis Face Family suidae Health Care Costs Healthcare Immune Immune response Immunity Immunization Immunologics Immunosuppressive Agents In Vitro Inbred BALB C Mice Invasive Investigation Label Life Lipids Liposomes Lyme Disease Malaria Malignant Neoplasms Methods Modeling Molecular Weight Morbidity - disease rate Mus Needles Numbers Penetration Phase Population Preparation Proteins Relative (related person)Risk Serum Skin Societies Specialist Stratum corneum System Techniques Technology Testing Therapeutic Tissue Transplantation Topical application Training Transdermal substance administration Tuberculosis Ultrasonography Vaccine Antigen Vaccines Vesicle Virus West Nile Fever anthrax protective factor base biodefense cost epidermis cell health care delivery immune function immunogenicity influenzavirus innovation killings mortality response uptake vaccine delivery

项目摘要

DESCRIPTION (provided by applicant): This objective of this investigation is to compare different liposome formulations for the ability to deliver vaccine antigens to the dendritic cells of the epidermis and initiate an effective immune response to target antigens following transcutaneous immunization. The choice of lipids used in liposome preparation should enhance penetration of the protein antigens through the stratum corneum into the epidermis, where immunologically-responsive cells exist. The first phase of the investigation will focus on uptake through the skin using an in vitro porcine model. Delivery of fluorescently-labeled liposomes and antigenic proteins to dendritic cells in the epidermis will be visualized as well. The second phase will determine the immunologic response, both serum and mucosal, to transcutaneously applied liposome-encapsulated Protective Antigen of Bacillus anthracis in a murine model. The immunogenicity of encapsulated versus free soluble protein will be compared. Lastly, the ability of a transcutaneously applied liposomal vaccine to protect immunized mice against an anthrax challenge will be assessed. Results will provide an understanding of influence of the lipid composition of liposomes on transport of proteins administered through the skin and the immunologic response to a transcutaneous liposomal vaccine, which could offer a new technique for protection against a number of infectious diseases.

描述(申请人提供)：这项研究的目的是比较不同的脂质体制剂将疫苗抗原输送到表皮树突状细胞的能力，并在经皮免疫后启动对目标抗原的有效免疫反应。脂质体制备中使用的脂类的选择应加强蛋白质抗原通过角质层进入表皮的渗透，表皮存在免疫反应细胞。研究的第一阶段将重点放在使用体外猪模型通过皮肤吸收。将荧光标记的脂质体和抗原蛋白输送到表皮中的树突状细胞也将被可视化。第二阶段将在小鼠模型中确定血清和粘膜对经皮应用脂质体包裹的炭疽杆菌保护性抗原的免疫反应。比较胶囊化和游离可溶性蛋白的免疫原性。最后，将评估经皮肤应用脂质体疫苗保护免疫小鼠免受炭疽病攻击的能力。研究结果将有助于了解脂质体的脂类成分对蛋白质经皮转运的影响，以及对经皮脂质体疫苗的免疫应答，为预防多种感染性疾病提供新的技术手段。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Improved dermal delivery of FITC-BSA using a combination of passive and active methods.

使用被动和主动方法的组合改善 FITC-BSA 的真皮递送。

DOI：
10.1002/jps.22687
发表时间：
2011
期刊：
Journal of pharmaceutical sciences
影响因子：
3.8
作者：
Wang,Qing;Jaimes-Lizcano,YulyA;Lawson,LouiseB;John,VijayT;Papadopoulos,KyriakosD
通讯作者：
Papadopoulos,KyriakosD

Hydration effects on skin microstructure as probed by high-resolution cryo-scanning electron microscopy and mechanistic implications to enhanced transcutaneous delivery of biomacromolecules.

DOI：
10.1002/jps.21863
发表时间：
2010-02
期刊：
JOURNAL OF PHARMACEUTICAL SCIENCES
影响因子：
3.8
作者：
Tan, Grace;Xu, Peng;Lawson, Louise B.;He, Jibao;Freytag, Lucia C.;Clements, John D.;John, Vijay T.
通讯作者：
John, Vijay T.

Highly aspherical silica nanoshells by templating tubular liposomes.

通过模板管状脂质体制备高度非球形二氧化硅纳米壳。