Histopathology Core B

组织病理学核心 B

• 批准号: 8596397
• 负责人: Qian-Chun Yu
• 金额: $ 19.82万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-18 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8596397
• 关键词: Address; Animals; Antigens; Cell Culture Techniques; Cell Death; Cells; Detection; Economics; Electron Microscopy; Histopathology; Hypoxia; Immunofluorescence Microscopy; Immunohistochemistry; Individual; Instruction; Malignant Neoplasms; Modeling; Morphology; Organelles; Pathway interactions; Proteins; Sampling; Services; Staining method; Stains; Tissues; Tumor Tissue; base; cell injury; neoplastic cell; response; tumor; tumor microenvironment

The Cell/Tissue Morphology Core (Core B) is strategically established to address the shared needs of morphological characterization of tumor samples atthe histological, cellular, and organelle levels. Based on the specific aims proposed by the 3 Project Leaders, this Core will have 2 essential components: (1) Histopathology to perform histological analysis of tumors, quantitative and qualitative assessment of cell death/survival pathways, and interactions between tumor cells and microenvironment from animal and cell culture models. The core will also perform quantitative immunohistochemistry (IHC) analysis of hypoxia and key UPR antigens identified by this programmatic group to assess the expression of critical molecules. (2) Electron Microscopy (EM) analysis of ultrastructural changes to cell organelles, particularly the changes of ER compartment and other secretory organelles, subcellular evidence of cell injury and cell death, and the dynamic changes of organelles involved in autophagic pathway. It is oiir strong believe that the Core B will be able to provide project-oriented technological support with consistence, high quality, and scientific interaction in a much more efficient and economic fasliion than that performed and maintained by each individual group. RELEVANCE (See instructions): CORE B is not a

细胞/组织形态学核心（核心B）是战略性建立的，以满足以下方面的共同需求 肿瘤样品在组织学、细胞和细胞器水平上的形态学特征。基于 根据3位项目负责人提出的具体目标，该核心将包括2个基本组成部分：（1） 组织学进行肿瘤组织学分析，细胞定量和定性评估 死亡/存活途径以及肿瘤细胞与动物和细胞微环境之间的相互作用 文化模式该核心还将对缺氧和缺氧进行定量免疫组织化学（MHC）分析， 该项目组确定的关键UPR抗原，以评估关键分子的表达。（二） 电镜分析细胞器超微结构的变化，特别是ER的变化 室和其他分泌细胞器，细胞损伤和细胞死亡的亚细胞证据， 参与自噬途径的细胞器的动态变化。 我们坚信，核心B将能够提供面向项目的技术支持， 一致性，高质量和科学的互动，在一个更有效和更经济的fasliion比， 由每个小组执行和维护。 相关性（参见说明）： CORE B不是