Histopathology Core B

组织病理学核心 B

• 批准号: 8918292
• 负责人: Qian-Chun Yu
• 金额: $ 14.85万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8918292
• 关键词: Address; Animals; Antigens; Cell Culture Techniques; Cell Death; Cells; Detection; Economics; Electron Microscopy; Histopathology; Hypoxia; Immunofluorescence Microscopy; Immunohistochemistry; Individual; Malignant Neoplasms; Modeling; Morphology; Organelles; Pathway interactions; Proteins; Protocols documentation; Sampling; Services; Staining method; Stains; Techniques; Technology; Testing; Tissues; Tumor Tissue; base; cell injury; member; neoplastic cell; programs; response; tumor; tumor microenvironment

The Cell/Tissue Morphology Core (Core B) is strategically established to address the shared needs of morphological characterization of tumor samples atthe histological, cellular, and organelle levels. Based on the specific aims proposed by the 3 Project Leaders, this Core will have 2 essential components: (1) Histopathology to perform histological analysis of tumors, quantitative and qualitative assessment of cell death/survival pathways, and interactions between tumor cells and microenvironment from animal and cell culture models. The core will also perform quantitative immunohistochemistry (IHC) analysis of hypoxia and key UPR antigens identified by this programmatic group to assess the expression of critical molecules. (2) Electron Microscopy (EM) analysis of ultrastructural changes to cell organelles, particularly the changes of ER compartment and other secretory organelles, subcellular evidence of cell injury and cell death, and the dynamic changes of organelles involved in autophagic pathway. It is oiir strong believe that the Core B will be able to provide project-oriented technological support with consistence, high quality, and scientific interaction in a much more efficient and economic fasliion than that performed and maintained by each individual group.

细胞/组织形态核心(核心B)是战略上建立的，以满足以下共同需求 肿瘤标本在组织学、细胞和细胞器水平上的形态特征。基于 3个项目负责人提出的具体目标，这个核心将有两个基本组成部分：(1) 组织病理学对肿瘤进行组织学分析，对细胞进行定量和定性评估 动物和细胞的死亡/生存途径以及肿瘤细胞与微环境的相互作用 文化典范。核心还将进行定量免疫组织化学(IHC)分析缺氧和 由这个程序组确定的关键UPR抗原，以评估关键分子的表达。(2) 细胞器超微结构变化，特别是内质网变化的电子显微镜分析 间室和其他分泌细胞器，细胞损伤和细胞死亡的亚细胞证据，以及 自噬途径中细胞器的动态变化。 我坚信，核心B将能够提供面向项目的技术支持 一致性、高质量和科学交互，比这更高效、更经济 由每个单独的小组执行和维护。