Analytical Pharmacology

项目总结（见说明）： 分析药理学核心设施（APCF）通过提供广泛的分析和咨询服务，鼓励和促进COHCCC基础科学家和临床医生之间的合作研究。APCF协助设计和进行临床和临床前研究的药代动力学研究。提供的主要服务有：1）药物、生物标志物和相关化合物的定量测定开发和样品分析（LCMS/ MS、GC/MS、AAS和HPLC），以及2）药代动力学和代谢物数据的研究设计和专家分析。APCF位于夏皮罗大楼，所有主要的分析设备都集中在一个相连的房间里。核心内使用最频繁的主要设备是3台LC-MS/MS仪器，包括Micromass Quattro Ultima三重四柱、沃茨Quattro Premier XE三重四柱和AB Sciex QTRAP 5500。这些仪器中的每一个都包括不同的HPLC组件，这些组件具有独特的功能，提供了更大的灵活性，并且每个系统都为复杂生物基质中的分析物提供了最先进的选择性和灵敏度。Micromass和沃茨系统的仪器控制和数据采集通过运行MassLynx和QuanLynx软件的单独MassLynx-NT工作站进行协调。MetaboLynx软件也可用于Quattro Premier XE，以帮助代谢物鉴定。QTRAP 5500的仪器控制和数据分析在运行Analyst软件的专用工作站上进行。其他核心设备包括：Shimadzu QP-5000 El型气相色谱仪/质谱仪，直接连接到运行CLASS-5000软件的专用PC; Perkin Elmer AAnalyst 300 AAS，带有HGA 800石墨炉，用于测定金属和含金属化合物;和三个完整的HPLC系统，其由六个溶剂输送模块（4个Shimadzu LC-IOA，2个Shimadzu LClOAD）组成。HPLC检测能力涵盖广泛的当前可用方法，包括UV/维斯（Shimadzu SPD-10 AV）、荧光（Shimadzu RF-10 A）、电化学（ESA型号51 OOA和5200 A）和光电二极管阵列（沃茨Acquity PDA）检测系统。在2010年7月1日至2011年6月30日期间，共开发或重新启动了13种分析方法，分析了2657份样本，并在报告期内处理了907份临床样本。

PROJECT SUMMARY (See instructions): The Analytical Pharmacology Core Facility (APCF) encourages and facilitates collaborative research among COHCCC basic scientists and clinicians by providing a wide range of analytical and consultative services. APCF assists with the design and conduct of pharmacokinetic studies for clinical and preclinical investigations. Primary services provided are: 1) quantitative assay development and sample analysis (LCMS/ MS, GC/MS, AAS, and HPLC) of drugs, biomarkers, and related compounds, and 2) study design and expert analysis of pharmacokinetic and metabolite data. APCF is located in the Shapiro Building and all of the major analytical equipment is consolidated into a contiguous suite of rooms. The most heavily utilized major equipment within the core are the 3 LC-MS/MS instruments, including a Micromass Quattro Ultima triple quad, a Waters Quattro Premier XE triple quad, and an AB Sciex QTRAP 5500. Each of these instruments includes different HPLC components with unique features that provide greater flexibility, and each of the systems provides state-of-the-art selectivity and sensitivity for analytes in complex biological matrices. Instrument control and data acquisition for the Micromass and Waters systems are coordinated through separate MassLynx-NT Workstations running MassLynx and QuanLynx software. MetaboLynx software is also available for the Quattro Premier XE to aid in metabolite identification. Instrument control and data analysis for the QTRAP 5500 is performed on a dedicated workstation running Analyst software. Additional core equipment includes: a Shimadzu Model QP-5000 El gas chromatograph/mass spectrometer, interfaced directly to a dedicated PC running CLASS-5000 software; a Perkin Elmer AAnalyst 300 AAS with an HGA 800 graphite furnace for determination of metals and metal containing compounds; and three complete HPLC systems consisting of six solvent delivery modules (4 Shimadzu LC-IOA's, 2 Shimadzu LClOAD's). HPLC detection capabilities cover a wide range of currently available methods, including UV/Vis (Shimadzu SPD-10AV), fluorescence (Shimadzu RF-10A), electrochemical (ESA models 51 OOA and 5200A), and photodiode array (Waters Acquity PDA) detection systems. Between July 1, 2010 and June 30, 2011, a total of 13 analytical methods were developed or re-initiated, 2657 samples analyzed, and 907 clinical samples processed during the reporting period.